| Size | Price | Stock | Qty |
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| 250mg |
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| 500mg |
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| 1g |
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Scopolamine (Hyoscine) is an approved medication used to treat motion sickness and postoperative nausea and vomiting. It acts as a competitive and non-selective antagonist of muscarinic acetylcholine receptor with an IC50 of 55.3 nM.
| ln Vitro |
In oocytes expressing 5-HT3-blocked oocytes, scopolamine did not elicit a response when applied alone; however, when scopolamine was applied concurrently with 2 μM 5-HT, the response was inhibited dependent on the concentration. With n = 6, the scopolamine pIC50 value is 5.68±0.05 (IC50=2.09) μM, the Hill Slope is 1.06±0.05, and the Kb is 3.23 μM. Scopolamine was applied during 5-HT administration, and the same concentration-dependent effects were seen. In order to conduct additional testing of competitive binding to the 5-HT3 receptor, [3H]granisetron, a well-known high-affinity competitive antagonist, was used to assess the competitiveness of unlabeled scopolamine. Scopolamine, with an average pKi of 5.17±0.24 (Ki=6.76 μM, n=3), shows concentration nutritional competition at 0.6 nM [3H]granisetron (~Kd)[1].
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| ln Vivo |
In animal models of Alzheimer's disease, scopolamine can be used to create models of the disease. In histopathological studies, there were no significant changes in the brain's histology; however, acetylcholinesterase (AchE) activity (7.98±0.065; P<0.001) in the hippocampal cells of molds that received scopolamine only in tap water compared with the normal group (3.06±0.296). Moreover, scopolamine-treated animals had significantly higher levels of malondialdehyde (MDA) (34.61±4.85; P<0.01) compared to the normal group (12.82±2.86). The scopolamine-treated group (0.3906±0.02) showed still prototype glutathione (GSH); in comparison to the normal group (43.21±3.46), the amyloid (Aβ1-42) concentration in the scop
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| References |
[1]. Lochner M, et al. The muscarinic antagonists Scopolamine and atropine are competitive antagonists at 5-HT3 receptors. Neuropharmacology. 2016 Sep;108:220-8.
[2]. O ET, et al. COGNITIVE-ENHANCING PROPERTIES OF MORINDA LUCIDA (RUBIACEAE) AND PELTOPHORUM PTEROCARPUM (FABACEAE) IN SCOPOLAMINE-INDUCED AMNESIC MICE. Afr J Tradit Complement Altern Med. 2017 Mar 1;14(3):136-141. [3]. Pattanashetti LA, et al. Evaluation of neuroprotective effect of Quercetin with Donepezil in Scopolamine-induced amnesia in rats. Indian J Pharmacol. 2017 Jan-Feb;49(1):60-64 |
| Molecular Formula |
C17H21NO4
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|---|---|
| Molecular Weight |
303.35
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| Exact Mass |
303.1471
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| CAS # |
51-34-3
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| Related CAS # |
Scopolamine hydrobromide;114-49-8;Scopolamine butylbromide;149-64-4;Scopolamine hydrobromide trihydrate;6533-68-2;Scopolamine hydrochloride;55-16-3
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| SMILES |
O1[C@]2([H])[C@@]3([H])C([H])([H])C([H])(C([H])([H])[C@@]([H])([C@]12[H])N3C([H])([H])[H])OC([C@@]([H])(C1C([H])=C([H])C([H])=C([H])C=1[H])C([H])([H])O[H])=O
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| Synonyms |
Scopolamine Transderm SCOP l-Scopolamine SEE
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~329.65 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (8.24 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (8.24 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (8.24 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.2965 mL | 16.4826 mL | 32.9652 mL | |
| 5 mM | 0.6593 mL | 3.2965 mL | 6.5930 mL | |
| 10 mM | 0.3297 mL | 1.6483 mL | 3.2965 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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