| Size | Price | Stock | Qty |
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| 10mg |
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| 25mg |
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| 50mg |
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| 100mg |
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| 250mg |
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| 500mg |
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| ln Vitro |
Protopine (10–40 μM, 24–96 h) limits the ability of translocated cells (HepG2, Huh7) to migrate, proliferate, and undergo EMT [2]. Protopine (10–40 μM, 24 h) suppresses PI3K/Akt signaling dye and causes cell opening in HepG2 and Huh7 cells via expressing caspase-3 and caspase-9 [2]. Protopine (0–10 μg/mL) inhibits serotonin transporter (SERT) function in S6 cells and N1 cells, while protopine (10–40 μM, 6 hours) increases the formation of reactive oxygen species (ROS) in HepG2 and Huh7 cells [3].
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| ln Vivo |
Protopine (0.1 and 1 mg/kg, intraperitoneal injection) can attenuate scopolamine (1 mg/kg)-induced memory impairment in mice [1]. Protropine (5-20 mg/kg, i.v.) inhibits tumor growth and inhibits PI3K/Akt, and induces caspase-3 channels in xenografted BALB/c mice (subcutaneously injected with HepG2 or Huh-7 cells) [ 2]. Pratopine (5-20 mg/kg, intraperitoneally) shows antidepressant-like effects in mouse HTR and TST tests [3]. Protopine (1-4 mg/kg, intraperitoneally, once daily for 3 days) has a protective effect against focal brain injury [4].
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| Cell Assay |
Western Blot Analysis[2]
Cell Types: HepG2, Huh7 Tested Concentrations: 10, 20, 40 μM Incubation Duration: 24 hrs (hours) Experimental Results: Induced caspase-3 and caspase-9 cleavage. Bcl-2 and Bcl-xl levels are diminished. Induces the release of the mitochondrial protein cytochrome c into the cytoplasm. |
| Animal Protocol |
Animal/Disease Models: 5-Hydroxy-DL-tryptophan (5-HTP)-induced mouse model [3]
Doses: 5, 10, 20 mg/kg Route of Administration: intraperitoneal (ip) injection Experimental Results: Increased 5-HTP-induced head Number of hemispheric twitch responses (HTR). Reduce the immobility time tested in the Tail Suspension Test (TST). |
| References |
[1]. Kim SR, et, al. Protopine from Corydalis ternata has anticholinesterase and antiamnesic activities. Planta Med. 1999 Apr;65(3):218-21.
[2]. Nie C, et, al. Protopine triggers apoptosis via the intrinsic pathway and regulation of ROS/PI3K/Akt signalling pathway in liver carcinoma. Cancer Cell Int. 2021 Jul 27;21(1):396. [3]. Xu LF, et al. Protopine inhibits serotonin transporter and noradrenaline transporter and has the antidepressant-like effect in mice models. Neuropharmacology. 2006 Jun;50(8):934-40. [4]. Xiao X, et al. Protective effect of protopine on the focal cerebral ischaemic injury in rats. Basic Clin Pharmacol Toxicol. 2007 Aug;101(2):85-9. |
| Molecular Formula |
C20H19NO5
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|---|---|
| Molecular Weight |
353.374
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| Exact Mass |
353.1263
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| CAS # |
130-86-9
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| Related CAS # |
Protopine hydrochloride;6164-47-2
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| SMILES |
O=C1CC2=CC=C3C(OCO3)=C2CN(C)CCC4=CC5=C(OCO5)C=C41
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| Synonyms |
Fumarine Biflorine Protopine
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~12.5 mg/mL (~35.37 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.47 mg/mL (4.16 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 14.7 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1.25 mg/mL (3.54 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 1.25 mg/mL (3.54 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.8299 mL | 14.1495 mL | 28.2990 mL | |
| 5 mM | 0.5660 mL | 2.8299 mL | 5.6598 mL | |
| 10 mM | 0.2830 mL | 1.4149 mL | 2.8299 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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