| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| Other Sizes |
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| ln Vitro |
PACAP-38(31–38), human, mouse, and rat TFA (100 nM; 2 min; NCI-H838 cells) tyrosine phosphorylates EGFR, HER2, and ERK[1]. Inducing EGFR tyrosine phosphorylation with PACAP-38(31–38), human, mouse, and rat TFA (100 nM; 30 min; NCI-H838 cells) raises ROS levels by 51%[1]. NCI-H838 cell growth is stimulated by TFA (10 nM; 48 h) in human, mouse, and rat PACAP-38(31–38)[1]. Human, mouse, and rat TFA (300 nM; 4 h) together with PACAP-38(31–38) increases the production of APPsα in brain cells[2]. Through cAMP accumulation and an increase in cytosolic free calcium, PACAP-38(31–38), human, mouse, and rat TFA(0.01–10 nM; HEK 293 cells) stimulates neural cells express endogenous PAC1 receptors. It also induces elevation of the intracellular Ca2+ concentration in a dose-dependent manner with an EC50 value of 0.81 nM[2]. PACAP-38(31–38), human, mouse, and rat TFA (0.01 nM; 48 h) stimulates NPY release from SCG neuronal cells in a strong and effective manner[3]. Human, mouse, and rat TFA (100 nM; 14 d) along with PACAP-38(31–38) generates prolonged activated NPY and catecholamine secretionmpathetic neurons[3].
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| Cell Assay |
Cell Viability Assay[1]
Cell Types: NCI-H838 cells Tested Concentrations: 10 nM Incubation Duration: 48 hrs (hours) Experimental Results: Increased the number of NCI-H838 cells by 72%. Western Blot Analysis[1] Cell Types: NCI-H838 cells Tested Concentrations: 100 nM Incubation Duration: 2 min Experimental Results: Increased tyrosine phosphorylation of the EGFR, HER2, and ERK by 377, 299 and 216%, respectively. |
| References |
[1]. Moody TW, et, al. PAC1 regulates receptor tyrosine kinase transactivation in a reactive oxygen species-dependent manner. Peptides. 2019 Oct;120:170017.
[2]. Kojro E, et, al. The neuropeptide PACAP promotes the alpha-secretase pathway for processing the Alzheimer amyloid precursor protein. FASEB J. 2006 Mar;20(3):512-4. [3]. Braas KM, et, al. Pituitary adenylate cyclase-activating polypeptides, PACAP-38 and PACAP-27, regulation of sympathetic neuron catecholamine, and neuropeptide Y expression through activation of type I PACAP/VIP receptor isoforms. Ann N Y Acad Sci. 1996 De |
| Molecular Formula |
C49H84F3N17O13
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|---|---|
| Molecular Weight |
1176.29
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| Related CAS # |
PACAP-38 (31-38), human, mouse, rat;138764-85-9
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O :~100 mg/mL (~85.01 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: 100 mg/mL (85.01 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 0.8501 mL | 4.2507 mL | 8.5013 mL | |
| 5 mM | 0.1700 mL | 0.8501 mL | 1.7003 mL | |
| 10 mM | 0.0850 mL | 0.4251 mL | 0.8501 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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