| Size | Price | |
|---|---|---|
| Other Sizes |
| Targets |
HMMNI does not have a specific pharmacological target. It is a metabolite of dimetridazole and serves as a biomarker for exposure to this veterinary drug. Its detection in agricultural samples indicates the use of dimetridazole in food-producing animals.
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|---|---|
| ln Vitro |
In vitro, HMMNI is used as an analytical reference standard for the detection of dimetridazole residues in food products. It does not exhibit significant biological activity. Its primary use is in the development and validation of analytical methods for food safety testing.
|
| ln Vivo |
In vivo, HMMNI is a metabolite formed from the breakdown of dimetridazole in animals. Its presence in tissues is used as a marker to monitor the use of this nitroimidazole drug in veterinary practice.
|
| Enzyme Assay |
Dimetridazole and its metabolites are extracted from samples and analyzed by LC-MS/MS. HMMNI is used as a reference standard to confirm the identity and quantify the concentration of the metabolite in the sample. The compound's chromatographic properties and mass spectrum are well-characterized.
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| Cell Assay |
For in vitro cell assays, HMMNI is not typically used as a treatment. It is used as a standard in analytical chemistry. Cell-based assays may be employed to study the metabolism of dimetridazole, but HMMNI itself is primarily a marker compound.
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| Animal Protocol |
For in vivo animal studies, HMMNI is studied as a metabolite of dimetridazole. Animals are administered dimetridazole, and tissue samples are collected. The compound is extracted and quantified by LC-MS to determine residue levels and assess compliance with food safety regulations.
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| ADME/Pharmacokinetics |
HMMNI (Hydroxy Dimetridazole) has the molecular formula C₅H₇N₃O₃ and a molecular weight of 157.13. It is a solid compound. It is soluble in organic solvents. The compound is stable under recommended storage conditions. It is also known as hydroxy dimetridazole.
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| Toxicity/Toxicokinetics |
The compound is for research use only. No specific toxicity data are available for HMMNI. Its significance lies in its role as a metabolite of a veterinary drug. Standard laboratory safety precautions should be followed when handling this compound.
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| References | |
| Additional Infomation |
HMMNI (CAS# 936-05-0) is the hydroxylated metabolite of the veterinary drug dimetridazole. It has not been approved as a therapeutic agent. The compound is used as an analytical standard in food safety testing for the detection of nitroimidazole drug residues in agricultural products. It is also known as Hydroxy Dimetridazole.
|
| Molecular Formula |
C5H7N3O3
|
|---|---|
| Molecular Weight |
157.13
|
| Exact Mass |
157.048
|
| CAS # |
936-05-0
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| Related CAS # |
HMMNI-d3;1015855-78-3
|
| PubChem CID |
557356
|
| Appearance |
Off-white to yellow solid powder
|
| Density |
1.5±0.1 g/cm3
|
| Boiling Point |
388.0±22.0 °C at 760 mmHg
|
| Melting Point |
110-112ºC
|
| Flash Point |
188.5±22.3 °C
|
| Vapour Pressure |
0.0±0.9 mmHg at 25°C
|
| Index of Refraction |
1.634
|
| LogP |
-0.49
|
| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
4
|
| Rotatable Bond Count |
1
|
| Heavy Atom Count |
11
|
| Complexity |
158
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
CN1C(=CN=C1CO)[N+](=O)[O-]
|
| InChi Key |
JSAQDPJIVQMBAY-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C5H7N3O3/c1-7-4(3-9)6-2-5(7)8(10)11/h2,9H,3H2,1H3
|
| Chemical Name |
(1-methyl-5-nitroimidazol-2-yl)methanol
|
| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO: 100 mg/mL (636.42 mM)
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (13.24 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (13.24 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 6.3642 mL | 31.8208 mL | 63.6416 mL | |
| 5 mM | 1.2728 mL | 6.3642 mL | 12.7283 mL | |
| 10 mM | 0.6364 mL | 3.1821 mL | 6.3642 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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