| Size | Price | Stock | Qty |
|---|---|---|---|
| 100g |
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| 250g |
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| Other Sizes |
| Targets |
MES sodium salt does not have a defined pharmacological target. It is a zwitterionic buffering agent used to maintain pH in biological and biochemical experiments. The compound exhibits poor affinity with metal ions (binding constants at 20°C and 0.1 M: Mg²⁺, 0.8; Ca²⁺, 0.8; Mn²⁺, 1.7), thereby can be used in studies involving metal ions.
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|---|---|
| ln Vitro |
In vitro, MES sodium salt is used as a buffering agent in biological and biochemical research including plant cell culture. It is easily dissolved in water, contains a mid range pKa, and has a useful pH range of 5.5 to 6.5. It is chemically stable with minimal absorption in both UV and visible spectral range. The compound is used in cell culture, electrophoresis, and capillary electrochromatography. It shows good buffering properties and poor affinity with metal ions.
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| ln Vivo |
In vivo data for MES sodium salt are not available, as the compound is a buffer reagent used exclusively in vitro. It is not intended for in vivo administration and has no established in vivo pharmacokinetic or pharmacodynamic profile. The compound is used in biological research applications but is not designed for therapeutic use.
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| Enzyme Assay |
For in vitro biochemical assays, MES sodium salt is used as a buffering agent to maintain pH in enzyme assays, cell culture media, and electrophoretic systems. The buffer is prepared by dissolving the sodium salt in deionized water at the desired concentration (typically 10-100 mM). The pH is adjusted with acid or base as needed. The compound's poor affinity with metal ions makes it suitable for studies involving metal ions. It is chemically stable with minimal UV/Vis absorption.
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| Cell Assay |
For in vitro cell-based experiments, MES sodium salt is used as a buffering agent in cell culture media. The compound is added to culture media at concentrations of 10-50 mM to maintain pH in the range of 5.5-6.7. It is compatible with most cell types and does not interfere with cell viability assays. The buffer is also used in electrophoretic and chromatographic applications.
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| Animal Protocol |
In vivo animal studies for MES sodium salt are not applicable, as the compound is a buffer reagent used exclusively for in vitro applications. No animal studies have been conducted for this compound, and it is not intended for therapeutic or diagnostic use in animals or humans.
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| ADME/Pharmacokinetics |
Pharmacokinetic data for MES sodium salt are not available, as the compound is not a drug and is not administered to living organisms. The compound is a zwitterionic buffer with a pKa in the range of 5.5-6.7. It is water-soluble, giving a clear colorless solution at 25 g in 50 mL water (approx. 2.3 M). It is chemically stable and has no therapeutic applications requiring pharmacokinetic characterization.
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| Toxicity/Toxicokinetics |
MES sodium salt is a low-toxicity biochemical reagent. As a zwitterionic buffer, it is generally considered to be non-toxic and non-irritating at the concentrations used in biological research (10-100 mM). The compound is not classified as hazardous under standard GHS criteria. However, standard laboratory safety practices should be followed, including the use of personal protective equipment and working in a well-ventilated area.
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| References |
[1]. N E Good, et al. Hydrogen ion buffers for biological research. Biochemistry. 1966 Feb;5(2):467-77.
[2]. Tomoko Kagenishi, et al. MES Buffer Affects Arabidopsis Root Apex Zonation and Root Growth by Suppressing Superoxide Generation in Root Apex. Front Plant Sci. 2016 Feb 18;7:79. |
| Additional Infomation |
Sodium 2-(N-morpholino)ethanesulfonate is the sodium salt of 2-(N-morpholino)ethanesulfonic acid, belonging to the category of organic sodium salts. It contains the 2-(N-morpholino)ethanesulfonate ion.
MES sodium salt (2-Morpholinoethanesulfonic acid sodium salt, CAS 71119-23-8) is a research-grade biochemical reagent, not an FDA-approved pharmaceutical drug. Its primary application is as a zwitterionic buffering agent for biological and biochemical research, including plant cell culture. The compound has a working pH range of 5.5-6.7, is chemically stable with minimal UV/Vis absorption, and exhibits poor affinity with metal ions. It is used in cell culture, electrophoresis, and capillary electrochromatography. |
| Molecular Formula |
C6H12NNAO4S
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|---|---|
| Molecular Weight |
217.22
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| Exact Mass |
217.038
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| CAS # |
71119-23-8
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| Related CAS # |
MES;4432-31-9;MES monohydrate;145224-94-8;MES potassium;39946-25-3;MES-d13;352534-94-2
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| PubChem CID |
23673676
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| Appearance |
White to off-white solid powder
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
13
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| Complexity |
219
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| Defined Atom Stereocenter Count |
0
|
| SMILES |
S(C([H])([H])C([H])([H])N1C([H])([H])C([H])([H])OC([H])([H])C1([H])[H])(=O)(=O)[O-].[Na+]
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| InChi Key |
IRHWMYKYLWNHTL-UHFFFAOYSA-M
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| InChi Code |
InChI=1S/C6H13NO4S.Na/c8-12(9,10)6-3-7-1-4-11-5-2-7;/h1-6H2,(H,8,9,10);/q;+1/p-1
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| Chemical Name |
sodium;2-morpholin-4-ylethanesulfonate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 25 mg/mL (115.09 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (11.51 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (11.51 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (11.51 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.6036 mL | 23.0181 mL | 46.0363 mL | |
| 5 mM | 0.9207 mL | 4.6036 mL | 9.2073 mL | |
| 10 mM | 0.4604 mL | 2.3018 mL | 4.6036 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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