| Size | Price | Stock | Qty |
|---|---|---|---|
| 5g |
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| 10g |
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| Other Sizes |
| Targets |
MES does not have a biological target; it functions as a chemical buffer. Its utility is based on its zwitterionic nature, midrange pKa, and minimal interaction with metal ions. It does not engage in complex formation with the majority of metals commonly used in biological studies. It is chemically and enzymatically stable, with minimal absorption in the visible or UV spectral range.
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|---|---|
| ln Vitro |
In vitro, MES is used to maintain a stable pH in various biochemical assays, particularly those requiring a slightly acidic environment (pH 5.5-6.7). It is used in plant culture media, reagent solutions, and physiological experiments. It is also used in protein characterization assays. It is not recommended for buffering at pH 7.4.
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| ln Vivo |
MES is not administered in vivo as a therapeutic agent. It is a research buffer. It can be used to adjust the pH of solutions that are subsequently used in animals, but it is not the active test article. It is not absorbed by cell membranes, which is a key feature for a biological buffer.
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| Enzyme Assay |
Non-cellular enzyme/receptor binding assays using MES involve preparing the buffer at the desired pH (typically 5.5-6.7) by titrating MES acid with NaOH. Alternatively, stock solutions of MES and its sodium salt can be mixed. The buffer is then used as the assay medium to maintain a constant, slightly acidic pH during the reaction, which is critical for enzymes that are active or stable at this pH range.
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| Cell Assay |
In vitro cell experiments with MES involve its use as a buffer component in cell culture media or assay buffers for experiments conducted at a slightly acidic pH. It is particularly useful in plant cell culture. However, for mammalian cell culture at physiological pH (7.2-7.4), other buffers like HEPES are more appropriate, as MES is not recommended for pH 7.4.
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| Animal Protocol |
MES is not used in in vivo animal experiments as a therapeutic agent. Its role is as a buffer for research applications. It may be used to prepare solutions or media for administration to animals, but it is not the compound of interest. Its safety profile is well-established for research use.
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| ADME/Pharmacokinetics |
MES is a small, zwitterionic molecule (MW 195.24 anhydrous) that is not metabolized in biological systems. It is highly water-soluble, with solutions stable at 2-8°C for months. It is not absorbed by cell membranes, which makes it an excellent buffer for maintaining extracellular pH without affecting intracellular processes. It can be sterilized by filtration but autoclaving is not recommended.
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| Toxicity/Toxicokinetics |
MES has very low toxicity and is considered biologically inert. It is not known to be cytotoxic. It is stable and does not interfere with biological reactions. Standard laboratory safety practices should be followed when handling. It is for R&D use only and not for drug, household, or other uses.
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| References | |
| Additional Infomation |
2-(N-morpholino)ethanesulfonic acid is a Goodyear buffer with a pKa of 6.15 at 20°C. It is an organic sulfonic acid and also a MES (methyl ethyl sulfonic acid). It is the conjugate acid of 2-(N-morpholino)ethanesulfonate and a tautomer of 2-(N-morpholinonyl)ethanesulfonate.
MES (4-Morpholineethanesulfonic acid) is a widely used biological buffer, particularly valuable for experiments requiring a pH below 7.0. Its low metal-binding affinity makes it suitable for studies involving metal ions. It is not a drug and has no clinical trials or approved therapeutic status. It is a standard reagent in biochemistry and plant biology laboratories. |
| Molecular Formula |
C6H13NO4S
|
|---|---|
| Molecular Weight |
195.24
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| Exact Mass |
195.056
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| CAS # |
4432-31-9
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| Related CAS # |
MES monohydrate;145224-94-8;MES sodium salt;71119-23-8;MES potassium;39946-25-3;MES-d13;352534-94-2
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| PubChem CID |
78165
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| Appearance |
White to off-white solid powder
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| Density |
1.4±0.1 g/cm3
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| Melting Point |
>300 °C(lit.)
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| Index of Refraction |
1.517
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| LogP |
-2.13
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| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
12
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| Complexity |
214
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C1COCCN1CCS(=O)(=O)O
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| InChi Key |
SXGZJKUKBWWHRA-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C6H13NO4S/c8-12(9,10)6-3-7-1-4-11-5-2-7/h1-6H2,(H,8,9,10)
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| Chemical Name |
2-morpholin-4-ylethanesulfonic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O: 125 mg/mL (640.24 mM)
DMSO: 50 mg/mL (256.10 mM) |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 50 mg/mL (256.10 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.1219 mL | 25.6095 mL | 51.2190 mL | |
| 5 mM | 1.0244 mL | 5.1219 mL | 10.2438 mL | |
| 10 mM | 0.5122 mL | 2.5610 mL | 5.1219 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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