Procarbazine HCl (NSC-77213)

Alias: NSC-77213 HCl; CB 400-497; NSC-77213; Ro 4-6467; CB 400497; NSC77213; Ro4-6467;CB-400-497; NSC77213; Ro4-6467; Ro 4-6467/1; Procarbazine Hydrochloride; PCB Hydrochloride; PCZ; Procarbazin; Matulane; Natulan; Natulanar; Natunalar

Cat No.:V1446 Purity: ≥98%

COA Product Data Instructions for use SDS

Procarbazine HCl (formerly NSC77213; Ro4-6467;CB-400-497; PCB; PCZ; Matulane; Natulanar), the hydrochloride salt form of procarbazine, is ananticancer chemotherapeutic medication approved for the treatment of Hodgkins lymphoma and certain brain cancers.

Procarbazine HCl (NSC-77213) Chemical Structure CAS No.: 366-70-1
Product category: DNA(RNA) Synthesis

This product is for research use only, not for human use. We do not sell to patients.

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Other Forms of Procarbazine HCl (NSC-77213):

Procarbazine (NSC-77213)

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Top Publications Citing lnvivochem Products

Nature 2021, 597(7874):119-125.

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J Am Chem Soc 2022,144:21831-21836.

Cell 2020,183:1202-1218.e25.

Science Adv 2022:8,eabm9427.

Nature 2021,597(7874):119-125.

Cell 2020,183:1202-1218.e25.

PNAS Nexus 2022,1(3):pgac063.

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Purity & Quality Control Documentation

Current batch：

Purity: ≥98%

COA

MSDS

NMR

Instructions

Product Description
Bioactivity & Protocols
Physicochemical Properties
Solubility Data
Calculators
Clinical Trial Information

Product Description

Procarbazine HCl (formerly NSC77213; Ro4-6467; CB-400-497; PCB; PCZ; Matulane; Natulanar), the hydrochloride salt form of procarbazine, is an anticancer chemotherapeutic medication approved for the treatment of Hodgkin's lymphoma and certain brain cancers.

Biological Activity I Assay Protocols (From Reference)

ln Vitro

Procarbazine plus Cu(II) induces lesions at the 5'-ACG-3' sequence, complementary to a p53 gene hotspot, and the 5'-TG-3' sequence that are formamidopyrimidine-DNA glycosylase-sensitive and piperidine-labile. Procarbazine damages DNA by generating methyl radicals and the Cu(I)-hydroperoxo complex non-enzymatically. [1] Procarbazine is mutagenic in a number of organs following high dose treatment and has a potent clastogenic effect in hematopoietic cells.[2]

ln Vivo

Procarbazine significantly reduces the weight of the testicles and epididyma, as well as haploid cells and spermatogenic arrest. These effects show variation in the test golden hamster population.[3] In brown adipose tissue, procarbazine causes a dose-dependent potentiation of MAO A. The elevation is more noticeable after monomethylhydrazine, with activity in rat homogenates reaching 350% of that in control homogenates. This amine's metabolism is decreased by procarbazine or monomethylhydrazine to an extent that is comparable to what was found ex vivo in blood vessel homogenates.[4] Procarbazine is a broad-spectrum carcinogen in rodents and monkeys, resulting in tumors of the nervous system, lung, mammary gland, and hematopoietic system. It is also mutagenic, clastogenic, and teratogenic in a variety of test systems of differing complexity. Under complex metabolic conditions, procarbazine in vivo produces a variety of chemically reactive species, such as free radicals and methylating agents.[5]

Cell Assay

Cell Line: L1210 cells
Concentration: 5 and 20 nM
Incubation Time: 1 hour
Result: Showed 99.3% and 99.9% survival of cells at 5 mM and 20 mM, respectively.

Animal Protocol

Male muta mouse (7–8 weeks old)
50 and 150 mg/kg
Intraperitoneal injection; 50 and 150 mg/kg; once daily; 5 days

References

[1]. Mutat Res . 2003 Aug 5;539(1-2):145-55.

[2]. Mutat Res . 1999 Aug 18;444(2):269-81.

[3]. Arch Androl . 2002 Mar-Apr;48(2):91-100.

[4]. J Pharm Pharmacol . 1995 Oct;47(10):837-45.

[5]. Carcinogenesis . 1997 Nov;18(11):2191-6.

These protocols are for reference only. InvivoChem does not independently validate these methods.

Physicochemical Properties

Molecular Formula

C12H19N3O.HCL

Molecular Weight

257.76

Exact Mass

221.15

Elemental Analysis

C, 55.92; H, 7.82; Cl, 13.75; N, 16.30; O, 6.21

CAS #

366-70-1

Related CAS #

671-16-9

Appearance

Solid powder

SMILES

CC(C)NC(=O)C1=CC=C(C=C1)CNNC.Cl

InChi Key

DERJYEZSLHIUKF-UHFFFAOYSA-N

InChi Code

InChI=1S/C12H19N3O.ClH/c1-9(2)15-12(16)11-6-4-10(5-7-11)8-14-13-3;/h4-7,9,13-14H,8H2,1-3H3,(H,15,16);1H

Chemical Name

4-[(2-methylhydrazinyl)methyl]-N-propan-2-ylbenzamide;hydrochloride

Synonyms

NSC-77213 HCl; CB 400-497; NSC-77213; Ro 4-6467; CB 400497; NSC77213; Ro4-6467;CB-400-497; NSC77213; Ro4-6467; Ro 4-6467/1; Procarbazine Hydrochloride; PCB Hydrochloride; PCZ; Procarbazin; Matulane; Natulan; Natulanar; Natunalar

HS Tariff Code

2934.99.9001

Storage

Powder -20°C 3 years

4°C 2 years

In solvent -80°C 6 months

-20°C 1 month

Shipping Condition

Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Solubility Data

Solubility (In Vitro)

DMSO: <1 mg/mL

Water: ~52 mg/mL (~201.7 mM)

Ethanol: ~52 mg/mL (~201.7 mM)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.8796 mL	19.3979 mL	38.7958 mL
	5 mM	0.7759 mL	3.8796 mL	7.7592 mL
	10 mM	0.3880 mL	1.9398 mL	3.8796 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.

Calculator

Mass

Concentration

Volume

Molecular Weight*

Calculate Reset

Molarity Calculator allows you to calculate the mass, volume, and/or concentration required for a solution, as detailed below:

Calculate the Mass of a compound required to prepare a solution of known volume and concentration
Calculate the Volume of solution required to dissolve a compound of known mass to a desired concentration
Calculate the Concentration of a solution resulting from a known mass of compound in a specific volume

See Example

An example of molarity calculation using the molarity calculator is shown below:
What is the mass of compound required to make a 10 mM stock solution in 5 ml of DMSO given that the molecular weight of the compound is 350.26 g/mol?

Enter 350.26 in the Molecular Weight (MW) box
Enter 10 in the Concentration box and choose the correct unit (mM)
Enter 5 in the Volume box and choose the correct unit (mL)
Click the “Calculate” button
The answer of 17.513 mg appears in the Mass box. In a similar way, you may calculate the volume and concentration.

Concentration (Start)

Volume (Start)

Concentration (End)

Volume (End)

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Dilution Calculator allows you to calculate how to dilute a stock solution of known concentrations. For example, you may Enter C1, C2 & V2 to calculate V1, as detailed below:

What volume of a given 10 mM stock solution is required to make 25 ml of a 25 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=25 μM, V2=25 ml and V1 is the unknown:

Enter 10 into the Concentration (Start) box and choose the correct unit (mM)
Enter 25 into the Concentration (End) box and select the correct unit (mM)
Enter 25 into the Volume (End) box and choose the correct unit (mL)
Click the “Calculate” button
The answer of 62.5 μL (0.1 ml) appears in the Volume (Start) box

Molecular formula

Total molecular weight

g/mol

Calculate Reset

Molecular Weight Calculator allows you to calculate the molar mass and elemental composition of a compound, as detailed below:

Note: Chemical formula is case sensitive: C12H18N3O4 c12h18n3o4
Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter the chemical/molecular formula and click the “Calculate’ button.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (or molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

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Reconstitution Calculator allows you to calculate the volume of solvent required to reconstitute your vial.

Enter the mass of the reagent and the desired reconstitution concentration as well as the correct units
Click the “Calculate” button
The answer appears in the Volume (to add to vial) box

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal to make allowance for loss during the experiment)

Dosage mg/kg

Average weight of animals g

Dosing volume per animal μL

Number of animals

Step 2: Enter in vivo formulation (This is only a calculator, not the exact formulation for a specific product. Please contact us first if there is no in vivo formulation in the solubility section.)

% DMSO

% Tween 80

% ddH₂O

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Calculation results

Working concentration： mg/mL；

Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O，mix and clarify.

Note： (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.

Clinical Trial Information

NCT Number	Recruitment	interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT00887146	Recruiting	Drug: procarbazine Drug: CCNU	Brain and Central Nervous System Tumors	Alliance for Clinical Trials in Oncology	September 2009	Phase 3
NCT03495960	Recruiting	Drug: Rituximab Drug: Procarbazine	Primary Central Nervous System Lymphoma	International Extranodal Lymphoma Study Group (IELSG)	June 15, 2019	Phase 2
NCT05425654	Recruiting	Drug: Rituximab, Methotrexate, Vincristine, Procarbazine, Lenalidomide	Primary CNS Lymphoma	National Research Center for Hematology, Russia	May 17, 2021	Phase 2