| Size | Price | Stock | Qty |
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| 250mg |
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| 500mg |
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| 1g |
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| 2g |
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| Other Sizes |
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| ln Vitro |
When L-canavanine sulfate (L-CAV) was given alone in arginine-rich media, it only slightly affected the cytotoxicity of HeLa, Hep G2, and SK-HEP-1 cells (IC50 values: 5 to 10 mM). L-canavanine sulfate's IC50 value in the HaCaT keratinocyte cell line was more than 10 mM, showing that it was not harmful to normal cells in vitro. The IC50 values of L-canavanine sulfate in HeLa, Hep G2, and SK-HEP-1 cells were 0.21±0.04, 0.64±0.16, and 1.18±0.14 mM in arginine-free media, respectively. When applied to HeLa and hepatocellular carcinoma cells, L-canavanine sulfate by itself has nearly little toxicity and can increase the cytotoxicity of vinblastine (VIN) and paclitaxel (PTX) [2].
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| ln Vivo |
L-canavanine sulfate (100 mg/kg) administration resulted in a complete attenuation of lipopolysaccharide-induced hypotension, a slight increase in mean arterial pressure of 20 mm Hg, and blood pressure close to basal levels. Eight hours following the treatment of L-canavanine sulfate (100 mg/kg), all endotoxemic rats (n = 7)[1] were administered lipopolysaccharide, with the exception of one. Li 210 leukemia animals live much longer when treated with L-canavanine, which suppresses DNA synthesis in Li 210 cells in vivo. A 44% increase in peak longevity was observed at the optimal dose of 18 g/kg. Due to medication toxicity, fatalities can happen at doses as low as 24 g/kg within the narrow therapeutic dose range [3].
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| References |
[1]. Teale DM, et al. L-canavanine restores blood pressure in a rat model of endotoxic shock. Eur J Pharmacol. 1994 Dec 12;271(1):87-92.
[2]. Nurcahyanti AD, et al. Cytotoxic potentiation of vinblastine and paclitaxel by L-canavanine in human cervical cancer and hepatocellular carcinoma cells. Phytomedicine. 2015 Dec 15;22(14):1232-7. [3]. Green MH, et al. Antitumor activity of L-canavanine against L1210 murine leukemia. Cancer Res. 1980 Mar;40(3):535-7 |
| Molecular Formula |
C5H13N4O3+
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|---|---|
| Molecular Weight |
177.18172
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| CAS # |
2219-31-0
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| Related CAS # |
L-Canavanine;543-38-4
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| SMILES |
N[C@@H](CCONC(N)=N)C(O)=O.O=S(O)(O)=O
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~100 mg/mL (~364.63 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: 50 mg/mL (182.32 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.6440 mL | 28.2199 mL | 56.4398 mL | |
| 5 mM | 1.1288 mL | 5.6440 mL | 11.2880 mL | |
| 10 mM | 0.5644 mL | 2.8220 mL | 5.6440 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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