KN-92 Phosphate

Alias:
Cat No.:V2802 Purity: ≥98%
KN-92 Phosphate, the phosphate salt of KN-92, is an inactive derivative of KN-93.
KN-92 Phosphate Chemical Structure CAS No.: 1135280-28-2
Product category: CaMK
This product is for research use only, not for human use. We do not sell to patients.
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Other Forms of KN-92 Phosphate:

  • KN-92 Hydrochloride
  • KN-92
Official Supplier of:
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Purity & Quality Control Documentation

Purity: ≥98%

Product Description

KN-92 Phosphate, the phosphate salt of KN-92, is an inactive derivative of KN-93. KN-92 is intended to be used as a control compound in studies designed to elucidate the antagonist activities of KN-93. KN-93 inhibits histamine-induced aminopyrine uptake in parietal cells (IC50 = 300 nM). KN-93 has been used to implicate roles for CaMKII in Ca2+-induced Ca2+ release in cardiac myocytes, constitutive phosphorylation of 5-lipoxygenase in 3T3 cells, and Ca2+-dependent activation of HIF-1α in colon cancer cell.

Biological Activity I Assay Protocols (From Reference)
ln Vitro
LX-2 cell growth is inhibited by KN-93 (5-50 μM; 24 hours), but KN-92 (5-50 μM; 24 hours) is ineffective in preventing cell growth[2]. KN-93, not KN-92, was found to decrease the expression of p53 and p21, according to an analysis of cell cycle regulator expression[2].
ln Vivo

Cell Assay
Cell Viability Assay[2]
Cell Types: Human hepatic stellate cells (LX-2)
Tested Concentrations: 5-50 μM
Incubation Duration: 24 hrs (hours)
Experimental Results: Ineffective in blocking cell growth.
Animal Protocol


References
[1]. Smyth JT, et al. Inhibition of the inositol trisphosphate receptor of mouse eggs and A7r5 cells by KN-93 via a mechanism unrelated to Ca2+/calmodulin-dependent protein kinase II antagonism. J Biol Chem. 2002;277(38):35061-35070.
[2]. An P, et al. KN-93, a specific inhibitor of CaMKII inhibits human hepatic stellate cell proliferation in vitro. World J Gastroenterol. 2007;13(9):1445-1448.
These protocols are for reference only. InvivoChem does not independently validate these methods.
Physicochemical Properties
Molecular Formula
C24H28CLN2O7PS
Molecular Weight
554.98
CAS #
1135280-28-2
SMILES
ClC1C=CC(=CC=1)/C=C/CN(C)CC1=CC=CC=C1NS(C1C=CC(=CC=1)OC)(=O)=O.P(=O)(O)(O)O
InChi Key
XRQHWVVDNMJDEQ-IPZCTEOASA-N
InChi Code
InChI=1S/C24H25ClN2O3S.H3O4P/c1-27(17-5-6-19-9-11-21(25)12-10-19)18-20-7-3-4-8-24(20)26-31(28,29)23-15-13-22(30-2)14-16-23;1-5(2,3)4/h3-16,26H,17-18H2,1-2H3;(H3,1,2,3,4)/b6-5+;
Chemical Name
(E)-N-(2-(((3-(4-chlorophenyl)allyl)(methyl)amino)methyl)phenyl)-4-methoxybenzenesulfonamide phosphate
Synonyms

KN-92 phosphate; KN-92; KN 92; KN92.

Storage

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

Shipping Condition
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
Solubility Data
Solubility (In Vitro)
DMSO: 25mg/mL
Water: N/A
Ethanol:N/A
Preparing Stock Solutions 1 mg 5 mg 10 mg
1 mM 1.8019 mL 9.0093 mL 18.0187 mL
5 mM 0.3604 mL 1.8019 mL 3.6037 mL
10 mM 0.1802 mL 0.9009 mL 1.8019 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.

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Note: Chemical formula is case sensitive: C12H18N3O4  c12h18n3o4
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Step 1: Enter information below (Recommended: An additional animal to make allowance for loss during the experiment)
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Calculation results

Working concentration mg/mL;

Method for preparing DMSO stock solution mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation:Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
             (2) Be sure to add the solvent(s) in order.

Biological Data
  • KN-92 Phosphate

    WST-8 assay showing the effect of KN-93 and KN-92 on the growth of LX-2 cells after incubation for 24 h (A) and at the indicated time (B).World J Gastroenterol.2007 Mar 7;13(9):1445-8.
  • KN-92 Phosphate

    Effects of KN-93 (A) and KN-92 (B) on expression of p53 in LX-2 cells.World J Gastroenterol.2007 Mar 7;13(9):1445-8.
  • KN-92 Phosphate

    Effects of KN-93 (A) and KN-92 (B) on expression of p21 in LX-2 cells.World J Gastroenterol.2007 Mar 7;13(9):1445-8.
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