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Purity: ≥98%
Ibuprofen (Dolgesic; Advil, Motrin, Nurofen, Brufen among others), a potent anti-inflammatory drug of the NSAID class, is an inhibitor of COX-1 and COX-2 enzymes with IC50s of 13 μM and 370 μM, respectively. Ibuprofen is an isobutylphenylpropanoic acid-based, and nonsteroidal anti-inflammatory drug (NSAID) used for treating pain, fever, and inflammation. This includes painful menstrual periods, migraines, and rheumatoid arthritis. In HCT-116 p53wt or HCT-116 p53-/-colon carcinoma cell lines, S- and R-ibuprofen induced apoptosis and blocked cell cycle is in part dependent on p53. The anti-proliferative effects were significantly higher in the p53wt cell line than in the p53-deficient cells.
| ln Vitro |
COX-1 and COX-2 activity is inhibited by ibuprofen (24 h) with IC50 values of 13 μM and 370 μM[1]. In AGS cells (adenocarcinoma gastric cell line), ibuprofen (500 μM, 48 h) causes apoptosis and suppresses angiogenesis and cell proliferation[2]. In AGS cells, ibuprofen (500 μM, 48 h) increases the RNA levels of wild type P53 and Bax genes but downregulates the transcription of Akt, VEGF-A, PCNA, Bcl2, OCT3/4, and CD44 genes[2]. In both primary CF nasal epithelial cells and cystic fibrosis (CF) cell models, ibuprofen (500 μM, 24 h) causes extension of microtubules to the cell periphery and restores microtubule-dependent intracellular cholesterol transport[3]. Through a photosensitization process, ibuprofen (500 μM, 24 h) increases UV-induced cell death in MCF-7 and MDA-MB-231 cells[4].
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| ln Vivo |
In a model of postpartum breast cancer, ibuprofen (300 mg/kg; po; daily, for 14 days) decreases overall tumor growth and improves anti-tumor immune features without causing harmful autoimmune reactions[5]. ?In rats with a chronic Oxaliplatin model, is there a reduction in the risk of neuropathy with Ibuprofen (60 mg/kg; ih; every other day for 15 days)?caused neuropathy in the periphery[6]. ?The average muscle fiber cross-sectional area is decreased by ibuprofen (20 mg/kg; po; every 12 hours, 5 doses total) without changing the regulation of supraspinatus tendon adaptations to exercise[7]. ?In a rat model of persistent lung infection, ibuprofen (35 mg/kg; po; twice daily) reduces the inflammatory response to pseudomonas aeruginosa[8].
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| Cell Assay |
Cell Viability Assay[2]
Cell Types: AGS cells Tested Concentrations: 100-1000 μM Incubation Duration: 24 h, 48 h Experimental Results: Inhibited AGS cell viability with IC50 values of 630 μM (trypan blue staining, 24 h), 456 μM (neutral red assay, 24 h), 549 μM (trypan blue staining, 48 h) and 408 μM (neutral red assay, 48 h). |
| Animal Protocol |
Animal/Disease Models: Syngeneic (D2A1) orthotopic Balb/c mouse model of PPBC (postpartum)[5]
Doses: 300 mg/kg, daily for 14 days Route of Administration: Fed in animal feedings (added to pulverized standard chow and mixed dry , then mixed with water, made into chow pellets and dried thoroughly) Experimental Results: Suppresed tumor growth, decreased presence of immature monocytes and increased numbers of T cells. Enhanced Th1 associated cytokines as well as promoted tumor border accumulation of T cells. Animal/Disease Models: Oxaliplatin‑induced peripheral neuropathy[6] Doses: 60 mg/kg, every second day for 15 days Route of Administration: subcutaneous (sc) injection Experimental Results: Lowered sensory nerve conduction velocity (SNCV). |
| References |
[1]. Noreen Y, et al. Development of a radiochemical cyclooxygenase-1 and -2 in vitro assay for identification of natural products as inhibitors of prostaglandin biosynthesis. J Nat Prod. 1998 Jan;61(1):2-7.
[2]. Hassan Akrami, et al. Inhibitory effect of ibuprofen on tumor survival and angiogenesis in gastric cancer cell. Tumour Biol. 2015 May;36(5):3237-43. [3]. Sharon M Rymut, et al. Ibuprofen regulation of microtubule dynamics in cystic fibrosis epithelial cells. Am J Physiol Lung Cell Mol Physiol. 2016 Aug 1;311(2):L317-27. [4]. Emmanuelle Bignon, et al. Ibuprofen and ketoprofen potentiate UVA-induced cell death by a photosensitization process. Sci Rep. 2017 Aug 21;7(1):8885. [5]. Nathan D Pennock, et al. Ibuprofen supports macrophage differentiation, T cell recruitment, and tumor suppression in a model of postpartum breast cancer. J Immunother Cancer. 2018 Oct 1;6(1):98. [6]. Thomas Krøigård, et al. Protective effect of ibuprofen in a rat model of chronic oxaliplatin-induced peripheral neuropathy. Exp Brain Res. 2019 Oct;237(10):2645-2651. [7]. Sarah Ilkhanipour Rooney, et al. Ibuprofen Differentially Affects Supraspinatus Muscle and Tendon Adaptations to Exercise in a Rat Model. Am J Sports Med. 2016 Sep;44(9):2237-45. [8]. M W Konstan, et al. Ibuprofen attenuates the inflammatory response to Pseudomonas aeruginosa in a rat model of chronic pulmonary infection. Implications for antiinflammatory therapy in cystic fibrosis. Am Rev Respir Dis. 1990 Jan;141(1):186-92. |
| Molecular Formula |
C13H18O2
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| Molecular Weight |
206.28
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| CAS # |
15687-27-1
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| Related CAS # |
(S)-(+)-Ibuprofen;51146-56-6;(S)-(+)-Ibuprofen-d3;1329643-44-8;(R)-(-)-Ibuprofen;51146-57-7;Ibuprofen sodium;31121-93-4;Ibuprofen-d3;121662-14-4;Ibuprofen-d4;Ibuprofen-13C6;1216459-54-9;Ibuprofen L-lysine;57469-77-9;Ibuprofen-13C,d3;1261394-40-4
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| SMILES |
O([H])C(C([H])(C([H])([H])[H])C1C([H])=C([H])C(=C([H])C=1[H])C([H])([H])C([H])(C([H])([H])[H])C([H])([H])[H])=O
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| InChi Key |
HEFNNWSXXWATRW-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C13H18O2/c1-9(2)8-11-4-6-12(7-5-11)10(3)13(14)15/h4-7,9-10H,8H2,1-3H3,(H,14,15)
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| Chemical Name |
2-[4-(2-methylpropyl)phenyl]propanoic acid
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| Synonyms |
Ibuprofen, Advil, Motrin, Nurofen, Brufen
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
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| Solubility (In Vivo) |
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| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.8478 mL | 24.2389 mL | 48.4778 mL | |
| 5 mM | 0.9696 mL | 4.8478 mL | 9.6956 mL | |
| 10 mM | 0.4848 mL | 2.4239 mL | 4.8478 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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