Histamine Phosphate

Alias: Histamine acid phosphate; Histamine diphosphate; Histamine phosphate; Histamine biphosphate; Histamine dihydrogen phosphate

Cat No.:V1227 Purity: ≥98%

COA Product Data Instructions for use SDS

Histamine (phosphate) is a strong histamine receptor agonist/activator and vasodilator neuroagent, capable of activating nitric oxide synthase.

Histamine Phosphate Chemical Structure CAS No.: 51-74-1
Product category: Histamine Receptor

This product is for research use only, not for human use. We do not sell to patients.

Size	Price	Stock	Qty
500mg
1g
2g
5g
10g
Other Sizes

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Other Forms of Histamine Phosphate:

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Top Publications Citing lnvivochem Products

Nature 2021, 597(7874):119-125.

Cell 2020,183:1202-1218.e25.

Science Adv 2022: 8, eabm9427.

Nature 597, 119–125 (2021)

Cell Stem Cell 2020,26(6):845-861.e12.

Science Trans Med 2023,15(701):eadd7872.

STTT 2023,8(1):91.

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Science Trans Med 2023, 15: eadd7872.

Cancer Cell 2021,39(4):529-547.e7.

Cancer Discov 2022,12(4):1106-1127.

Immunity 2023,56(3):620-634.e11.

J Am Chem Soc 2022,144:21831-21836.

Cell 2020,183:1202-1218.e25.

Science Adv 2022:8,eabm9427.

Nature 2021,597(7874):119-125.

Cell 2020,183:1202-1218.e25.

PNAS Nexus 2022,1(3):pgac063.

ACS Nano 2023,17(10):9110-9125.

Biomaterial 2023 Sep16:302:122333.

Purity & Quality Control Documentation

Current batch：

Purity: ≥98%

COA

MSDS

NMR

Instructions

Product Description
Bioactivity & Protocols
Physicochemical Properties
Solubility Data
Calculators
Clinical Trial Information

Product Description

Histamine (phosphate) is a strong histamine receptor agonist/activator and vasodilator neuroagent, capable of activating nitric oxide synthase.

Biological Activity I Assay Protocols (From Reference)

Targets

Histamine H1 receptor; Histamine H2 receptor

ln Vitro

In vitro activity: Histamine (10 μM) results in a greater build-up of inositol monophosphate in the chromaffin cells of the cow adrenal gland. The amount of radioactivity in the InsP3-containing fraction of bovine adrenal chromaffin cells is stimulated by histamine (10 μM). Compared to angiotensin I1 and bradykinin, histamine (100 μM) stimulates incorporation into the InsP3-containing eluate less. [1]

ln Vivo

Histamine phosphate (0.025 mg/kg) produces a mean increase in basilar blood flow of 145% of control in dogs. Histamine phosphate produces considerable increases in basilar blood flow as well as a decrease in femoral arterial blood pressure in dogs when injected intravenously and measured with an electromagnetic flow transducer. [2] In sheep that are not anesthetized, histamine phosphate (4 μg/kg) causes lymph flow to increase from 6.0 to 27.0 (SEM) ml/h. In addition, unanesthetized sheep treated with histamine phosphate (4 μg/kg) had decreases in cardiac output and arterial PO2 and increases in lung water, pulmonary vascular resistance, arterial PCO2, pH, and hematocrit. [3] In dogs that are anesthetized open-chested, histamine phosphate (8.3 mg/kg/min) has no discernible effect on pulmonary lymph flow (QL) or protein concentration (CL); nevertheless, both increase following alloxan. In anesthetized dogs with open chests, histamine phosphate (8.3 mg/kg/min) also has no discernible effect on the maximum capillary pressure (PCcritical) or the pulmonary capillary membrane filtration coefficient (Kf). [4] In the unanesthetized intact rat, histamine phosphate (50 mg/kg) causes a noticeable increase in acid secretion, but does not affect pepsin output. In the unanesthetized intact rat, histamine phosphate (50 mg/kg) stimulates gastric acid secretion to the greatest extent possible without causing any harmful side effects. [5]

Animal Protocol

8.3 mg/kg

Rat

References

[1]. J Neurochem . 1988 Aug;51(2):634-41.

[2]. Stroke . 1971 Jul-Aug;2(4):409-15.

[3]. J Clin Invest . 1976 Aug;58(2):391-8.

[4]. Am J Physiol . 1980 Jul;239(1):H96-100.

[5]. Gut . 1961 Mar;2(1):32-6.

These protocols are for reference only. InvivoChem does not independently validate these methods.

Physicochemical Properties

Molecular Formula	C5H15N3O8P2
Molecular Weight	307.14
Exact Mass	307.03
Elemental Analysis	C, 19.55; H, 4.92; N, 13.68; O, 41.67; P, 20.17
CAS #	51-74-1
Related CAS #	Histamine dihydrochloride; 56-92-8; Histamine; 51-45-6
Appearance	Solid powder
SMILES	C1=C(NC=N1)CCN.OP(=O)(O)O.OP(=O)(O)O
InChi Key	ZHIBQGJKHVBLJJ-UHFFFAOYSA-N
InChi Code	InChI=1S/C5H9N3.2H3O4P/c6-2-1-5-3-7-4-8-5;21-5(2,3)4/h3-4H,1-2,6H2,(H,7,8);2(H3,1,2,3,4)
Chemical Name	2-(1H-imidazol-5-yl)ethanamine;phosphoric acid
Synonyms	Histamine acid phosphate; Histamine diphosphate; Histamine phosphate; Histamine biphosphate; Histamine dihydrogen phosphate
HS Tariff Code	2934.99.9001
Storage	Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Solubility Data

Solubility (In Vitro)

DMSO: ~1.4 mg/mL (4.7 mM)

Water: ~42 mg/mL (~136.7 mM)

Ethanol: <1 mg/mL

Solubility (In Vivo)

Solubility in Formulation 1: 50 mg/mL (162.79 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.

(Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.2558 mL	16.2792 mL	32.5584 mL
	5 mM	0.6512 mL	3.2558 mL	6.5117 mL
	10 mM	0.3256 mL	1.6279 mL	3.2558 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.

Calculator

Mass

Concentration

Volume

Molecular Weight*

Calculate Reset

Molarity Calculator allows you to calculate the mass, volume, and/or concentration required for a solution, as detailed below:

Calculate the Mass of a compound required to prepare a solution of known volume and concentration
Calculate the Volume of solution required to dissolve a compound of known mass to a desired concentration
Calculate the Concentration of a solution resulting from a known mass of compound in a specific volume

See Example

An example of molarity calculation using the molarity calculator is shown below:
What is the mass of compound required to make a 10 mM stock solution in 5 ml of DMSO given that the molecular weight of the compound is 350.26 g/mol?

Enter 350.26 in the Molecular Weight (MW) box
Enter 10 in the Concentration box and choose the correct unit (mM)
Enter 5 in the Volume box and choose the correct unit (mL)
Click the “Calculate” button
The answer of 17.513 mg appears in the Mass box. In a similar way, you may calculate the volume and concentration.

Concentration (Start)

Volume (Start)

Concentration (End)

Volume (End)

Calculate Reset

Dilution Calculator allows you to calculate how to dilute a stock solution of known concentrations. For example, you may Enter C1, C2 & V2 to calculate V1, as detailed below:

What volume of a given 10 mM stock solution is required to make 25 ml of a 25 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=25 μM, V2=25 ml and V1 is the unknown:

Enter 10 into the Concentration (Start) box and choose the correct unit (mM)
Enter 25 into the Concentration (End) box and select the correct unit (mM)
Enter 25 into the Volume (End) box and choose the correct unit (mL)
Click the “Calculate” button
The answer of 62.5 μL (0.1 ml) appears in the Volume (Start) box

Molecular formula

Total molecular weight

g/mol

Calculate Reset

Molecular Weight Calculator allows you to calculate the molar mass and elemental composition of a compound, as detailed below:

Note: Chemical formula is case sensitive: C12H18N3O4 c12h18n3o4
Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter the chemical/molecular formula and click the “Calculate’ button.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (or molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

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Reconstitution Calculator allows you to calculate the volume of solvent required to reconstitute your vial.

Enter the mass of the reagent and the desired reconstitution concentration as well as the correct units
Click the “Calculate” button
The answer appears in the Volume (to add to vial) box

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal to make allowance for loss during the experiment)

Dosage mg/kg

Average weight of animals g

Dosing volume per animal μL

Number of animals

Step 2: Enter in vivo formulation (This is only a calculator, not the exact formulation for a specific product. Please contact us first if there is no in vivo formulation in the solubility section.)

% DMSO

% Tween 80

% ddH₂O

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Calculation results

Working concentration： mg/mL；

Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O，mix and clarify.

Note： (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.

Clinical Trial Information

NCT Number	Recruitment	interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT05131555	Active Recruiting	Drug: Placebo: Placebo D+ exercise training Drug: H1 blockade: H1 receptor Dantagonist + exercise training	Exercise Histamine	University Ghent	August 16, 2021	Not Applicable
NCT00362999	Active Recruiting	N/A	Allergic Rhinitis	Children's Mercy Hospital Kansas City	August 2006	N/A
NCT06154824	Recruiting	Other: Histamine Other: Cowhage	Histamine Cowhage	Aalborg University	December 15, 2023	Not Applicable
NCT06081998	Recruiting	Other: Histamine Other: Cowhage	Histamine Cowhage	Aalborg University	November 1, 2023	Not Applicable
NCT06081946	Completed	Other: Histamine Other: Cowhage	Histamine Cowhage	Aalborg University	December 15, 2023	Not Applicable