Histamine 2HCl (Ergamine)

Alias: Histamine dihydrochloride

Cat No.:V1224 Purity: ≥98%

COA Product Data Instructions for use SDS

Histamine 2HCl(Ergamine),the dihydrochloride salt of histamine, is an organic nitrogen compound and an endogenous metabolite involved in local immune responses, as well as regulating physiological function in the gut and acting as a neurotransmitter for the brain, spinal cord, and uterus.

Histamine 2HCl (Ergamine) Chemical Structure CAS No.: 56-92-8
Product category: Histamine Receptor

This product is for research use only, not for human use. We do not sell to patients.

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500mg
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5g
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25g
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Other Forms of Histamine 2HCl (Ergamine):

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Top Publications Citing lnvivochem Products

Nature 2021, 597(7874):119-125.

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Cancer Discov 2022,12(4):1106-1127.

Immunity 2023,56(3):620-634.e11.

J Am Chem Soc 2022,144:21831-21836.

Cell 2020,183:1202-1218.e25.

Science Adv 2022:8,eabm9427.

Nature 2021,597(7874):119-125.

Cell 2020,183:1202-1218.e25.

PNAS Nexus 2022,1(3):pgac063.

ACS Nano 2023,17(10):9110-9125.

Biomaterial 2023 Sep16:302:122333.

Purity & Quality Control Documentation

Current batch：

Purity: ≥98%

COA

MSDS

NMR

Instructions

Product Description
Bioactivity & Protocols
Physicochemical Properties
Solubility Data
Calculators
Clinical Trial Information

Product Description

Histamine 2HCl (Ergamine), the dihydrochloride salt of histamine, is an organic nitrogen compound and an endogenous metabolite involved in local immune responses, as well as regulating physiological function in the gut and acting as a neurotransmitter for the brain, spinal cord, and uterus.

Biological Activity I Assay Protocols (From Reference)

Targets

Histamine H1 receptor; Histamine H2 receptor

ln Vitro

Histamine inhibits the production of reactive oxygen species (ROS) via the Histaminetype-2 receptor (H2 receptor).[1] During a respiratory burst, histamine prevents monocytes/macrophages (MO) from producing and releasing reactive oxygen species (ROS). NK cell cytotoxicity (NKCC) is triggered by the combined effects of histamine and interleukin-2 (IL-2). By shielding immune system cells from oxidative stress and promoting endogenous immune cytotoxicity, histamine in combination with IL-2 may enhance response rates and disease-free survival.[2]

ln Vivo

In a rat model of early alcohol-induced liver injury, histamine treatment (0.5 mg/kg or 5.0 mg/kg, twice daily) significantly reduced liver pathology scores and preserved against liver injury as demonstrated by normal serum transaminase levels. An H2 receptor antagonist called ranitidine (10 mg/kg) blocks the protective effect of histamine, suggesting that the H2 receptor is the primary pathway through which the histamine effect is mediated. (Source: ) In male rats, histamine (30 pg/rat, icv) increases the concentrations of 3,4-dihydroxyphenylalanine acid and 3,4-dihydroxyphenylalanine accumulation in the nucleus accumbens. This effect is unaffected by the H2 antagonist zolantidine, suggesting that histamine stimulates mesolimbic DA neurons via an action at the H1 receptor.[3] In comparison to rats given subcutaneous saline injections, histamine (0.5 mg/kg s.c.) reduces the weight of liver tumors by 46% and subcutaneous tumors by 41%. The anti-tumour effect observed by subcutaneous histamine injections is inhibited by Ranitidine (50 mg/kg s.c.) in rats sarcoma.[4] When administered subcutaneously to Sprague-Dawley rats, histamine (1000 mg/kg s.c.) causes acute tissue damage after 24 hours and shows signs of pathological inflammation at the injection sites after 5 and 28 days. Histamine (1000 mg/kg s.c.) results in C_max of 167 mM, t_max of 0.5 hour, t_1/2 of 0.95 and AUC of 186 mmol-h/L in male Sprague-Dawley rats.[5]

Animal Protocol

0.5 mg/kg or 5.0 mg/kg

Rats

References

[1]. Inflammation . 2003 Oct;27(5):317-27.

[2]. Expert Opin Biol Ther . 2001 Sep;1(5):869-79.

[3]. Naunyn Schmiedebergs Arch Pharmacol . 1993 Jan;347(1):50-4.

[4]. Anticancer Res . 2002 Jul-Aug;22(4):1943-8.

[5]. Drug Chem Toxicol . 2003 Feb;26(1):35-49.

These protocols are for reference only. InvivoChem does not independently validate these methods.

Physicochemical Properties

Molecular Formula

C5H11CL2N3

Molecular Weight

184.07

Exact Mass

183.03

CAS #

56-92-8

Related CAS #

Histamine phosphate; 51-74-1; Histamine; 51-45-6

Appearance

White to off-white Solid powder

SMILES

C1=C(NC=N1)CCN.Cl.Cl

InChi Key

PPZMYIBUHIPZOS-UHFFFAOYSA-N

InChi Code

InChI=1S/C5H9N3.2ClH/c6-2-1-5-3-7-4-8-5;;/h3-4H,1-2,6H2,(H,7,8);2*1H

Chemical Name

2-(1H-imidazol-5-yl)ethanamine;dihydrochloride

Synonyms

Histamine dihydrochloride

Storage

Powder -20°C 3 years

4°C 2 years

In solvent -80°C 6 months

-20°C 1 month

Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.

Shipping Condition

Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Solubility Data

Solubility (In Vitro)

DMSO: 3~100 mg/mL (16.3~543.3 mM)

Water: ~37 mg/mL (~201.0 mM)

Ethanol: <1 mg/mL

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (13.58 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL.
Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution.

Solubility in Formulation 2: ≥ 2.5 mg/mL (13.58 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.

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Solubility in Formulation 3: ≥ 2.5 mg/mL (13.58 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.

Solubility in Formulation 4: 100 mg/mL (543.27 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication.

(Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	5.4327 mL	27.1636 mL	54.3272 mL
	5 mM	1.0865 mL	5.4327 mL	10.8654 mL
	10 mM	0.5433 mL	2.7164 mL	5.4327 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.

Calculator

Mass

Concentration

Volume

Molecular Weight*

Calculate Reset

Molarity Calculator allows you to calculate the mass, volume, and/or concentration required for a solution, as detailed below:

Calculate the Mass of a compound required to prepare a solution of known volume and concentration
Calculate the Volume of solution required to dissolve a compound of known mass to a desired concentration
Calculate the Concentration of a solution resulting from a known mass of compound in a specific volume

See Example

An example of molarity calculation using the molarity calculator is shown below:
What is the mass of compound required to make a 10 mM stock solution in 5 ml of DMSO given that the molecular weight of the compound is 350.26 g/mol?

Enter 350.26 in the Molecular Weight (MW) box
Enter 10 in the Concentration box and choose the correct unit (mM)
Enter 5 in the Volume box and choose the correct unit (mL)
Click the “Calculate” button
The answer of 17.513 mg appears in the Mass box. In a similar way, you may calculate the volume and concentration.

Concentration (Start)

Volume (Start)

Concentration (End)

Volume (End)

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Dilution Calculator allows you to calculate how to dilute a stock solution of known concentrations. For example, you may Enter C1, C2 & V2 to calculate V1, as detailed below:

What volume of a given 10 mM stock solution is required to make 25 ml of a 25 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=25 μM, V2=25 ml and V1 is the unknown:

Enter 10 into the Concentration (Start) box and choose the correct unit (mM)
Enter 25 into the Concentration (End) box and select the correct unit (mM)
Enter 25 into the Volume (End) box and choose the correct unit (mL)
Click the “Calculate” button
The answer of 62.5 μL (0.1 ml) appears in the Volume (Start) box

Molecular formula

Total molecular weight

g/mol

Calculate Reset

Molecular Weight Calculator allows you to calculate the molar mass and elemental composition of a compound, as detailed below:

Note: Chemical formula is case sensitive: C12H18N3O4 c12h18n3o4
Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter the chemical/molecular formula and click the “Calculate’ button.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (or molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

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Reconstitution Calculator allows you to calculate the volume of solvent required to reconstitute your vial.

Enter the mass of the reagent and the desired reconstitution concentration as well as the correct units
Click the “Calculate” button
The answer appears in the Volume (to add to vial) box

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal to make allowance for loss during the experiment)

Dosage mg/kg

Average weight of animals g

Dosing volume per animal μL

Number of animals

Step 2: Enter in vivo formulation (This is only a calculator, not the exact formulation for a specific product. Please contact us first if there is no in vivo formulation in the solubility section.)

% DMSO

% Tween 80

% ddH₂O

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Calculation results

Working concentration： mg/mL；

Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O，mix and clarify.

Note： (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.

Clinical Trial Information

NCT Number	Recruitment	interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT05131555	Active Recruiting	Drug: Placebo: Placebo D+ exercise training Drug: H1 blockade: H1 receptor Dantagonist + exercise training	Exercise Histamine	University Ghent	August 16, 2021	Not Applicable
NCT00362999	Active Recruiting	N/A	Allergic Rhinitis	Children's Mercy Hospital Kansas City	August 2006	N/A
NCT06154824	Recruiting	Other: Histamine Other: Cowhage	Histamine Cowhage	Aalborg University	December 15, 2023	Not Applicable
NCT06081998	Recruiting	Other: Histamine Other: Cowhage	Histamine Cowhage	Aalborg University	November 1, 2023	Not Applicable
NCT06081946	Completed	Other: Histamine Other: Cowhage	Histamine Cowhage	Aalborg University	December 15, 2023	Not Applicable