Fluoxetine HCl (LY-110140)

Alias: Lilly110140; Lilly-110140; LY-110140; Lilly 110140; Fluoxetine; Prozac; Sarafem; Fluoxetine; Animex-On; Fluoxetin; Pulvules; Eufor; Portal; LY110140; LY 110140
Cat No.:V0965 Purity: ≥98%
Fluoxetine HCl (LY-110140; Lilly-110140; Prozac, Sarafem, Animex-On, Pulvules, Eufor, Portal), the HCl salt of fluoxetine, is a potent and selective serotonin-reuptake inhibitor (SSRI) at the neuronal membrane with anti-depressant activity.
Fluoxetine HCl (LY-110140) Chemical Structure CAS No.: 56296-78-7
Product category: 5-HT Receptor
This product is for research use only, not for human use. We do not sell to patients.
Size Price Stock Qty
100mg
250mg
500mg
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Other Forms of Fluoxetine HCl (LY-110140):

  • Fluoxetine
  • (S)-Fluoxetine hydrochloride (S-isomer of fluoxetine)
  • (R)-Fluoxetine hydrochloride ((R)-Fluoxetine hydrochloride)
Official Supplier of:
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Purity & Quality Control Documentation

Purity: ≥98%

Product Description

Fluoxetine HCl (LY-110140; Lilly-110140; Prozac, Sarafem, Animex-On, Pulvules, Eufor, Portal), the HCl salt of fluoxetine, is a potent and selective serotonin-reuptake inhibitor (SSRI) at the neuronal membrane with anti-depressant activity. It can be used to treat premenstrual dysphoric disorder, bulimia nervosa, panic disorder, depression, and obsessive-compulsive disorder. In people 65 years of age and older, fluoxetine may reduce the risk of suicide. Premature ejaculation has also been treated with fluoxetine. It is consumed orally.

Biological Activity I Assay Protocols (From Reference)
Targets
5-HT
ln Vitro

In vitro activity: Fluoxetine inhibits the downregulation of cell proliferation brought on by the hippocampal cell's irreversible shock (IS).[1]
Fluoxetine increases the quantity of newborn cells in the dentate gyrus of the adult rat hippocampal hippocampus. Fluoxetine also boosts the quantity of proliferating cells in the prelimbic cortex.[2] Neurons in an immature state mature more quickly when taking fluoxetine. In the dentate gyrus, fluoxetine improves neurogenesis-dependent long-term potentiation (LTP).[3]
Fluoxetine, but not citalopram, fluvoxamine, paroxetine and sertraline, increases norepinephrine and dopamine extracellular levels in prefrontal cortex. After acute systemic administration, fluoxetine causes strong and long-lasting increases in extracellular concentrations of dopamine and norepinephrine.[4]

ln Vivo
Fluoxetine treatment also reverses the deficit in escape latency seen in animals exposed to inescapable shock in adult male Sprague–Dawley rats.[1] Extracellular levels of norepinephrine ([NE](ex)) and dopamine ([DA](ex)) are robustly and sustainably increased by fluoxetine and olanzapine up to 361% and 272% of the baseline, respectively. These increases are significantly higher than those produced by either medication alone.[5]
Cell Assay
The membrane currents induced by serotonin (5-hydroxytryptamine; 5HT) were inhibited by micromolar concentrations of fluoxetine (Prozac) in Xenopusoocytes expressing either cloned 5HT2C receptors or 5HT receptors. The IC50 of fluoxetine was approximately 20 μM for responses elicited by 1 μM 5-HT. Moreover, [3H]5HT binding to 5HT receptors in rat cortex membranes and [3H]5HT binding to 5HT2C receptors expressed in HeLa cells were both inhibited by fluoxetine, with Ki values of ≈65–97 nM and ≈ 56 μM, respectively. The administration of fluoxetine prevented the inescapable shock (IS)-induced downregulation of hippocampal cell proliferation, which led to a behaviorally hopeless state. In rat adult hippocampal dentate gyrus, fluoxetine augmented the number of proliferating cells. The prelimbic cortex's proliferating cell count was also elevated by fluoxetine. Neutrophils matured more quickly when taking fluoxetine. LTP in the dentate gyrus was enhanced by fluoxetine in a neurogenesis-dependent manner. In prefrontal cortex, fluoxetine increased norepinephrine and dopamine extracellular levels, but not other selective serotonin uptake inhibitors like citalopram, fluvoxamine, paroxetine, and sertraline. Fluoxetine generated significant and long-lasting increases in dopamine and norepinephrine extracellular concentrations following acute systemic administration.
Animal Protocol
Male Sprague-Dawley rats, weighing between 250 and 300 grams, are kept in housing with a 12-hour light/dark cycle (lights on at 7:00 am and lights off at 7:00 pm), constant humidity, and unrestricted access to food and water. For long-term medication therapy, rats receive intraperitoneal (IP) injections of fluoxetine (5 mg/kg/day) or saline, along with olanzapine or vehicle in their drinking water for 21 days (vehicle-treated control, fluoxetine, and olanzapine alone, as well as fluoxetine plus olanzapine). Olanzapine is the drug of choice for combination therapy because fluoxetine has the ability to disrupt olanzapine's metabolism and increase blood levels of the drug by up to 4-6 times. Olanzapine is dissolved in hydrochloric acid (HCl), and the stock solution with a concentration of 3 mg/mL is made by adjusting the pH back to 6 with 1 N sodium hydroxide. For the control animals, the same volume of vehicle solution is added to the water. Three times a week, the amount of fluid consumed is measured, and drinking bottles are refilled with pharmaceutical solution. The amount of fluid consumed by each treatment group is the same. The same dosage schedule applies to subchronic treatment, but the duration is extended to seven days.
References

[1]. Neuropsychopharmacology . 2003 Sep;28(9):1562-71.

[2]. Biol Psychiatry . 2004 Oct 15;56(8):570-80.

[3]. J Neurosci . 2008 Feb 6;28(6):1374-84.

[4]. Psychopharmacology (Berl) . 2002 Apr;160(4):353-61.

[5]. Neuropsychopharmacology . 2000 Sep;23(3):250-62.

[6]. Sci Rep . 2021 Jan 13;11(1):1250.

These protocols are for reference only. InvivoChem does not independently validate these methods.
Physicochemical Properties
Molecular Formula
C17H19CLF3NO
Molecular Weight
345.79
Exact Mass
345.11
Elemental Analysis
C, 59.05; H, 5.54; Cl, 10.25; F, 16.48; N, 4.05; O, 4.63
CAS #
56296-78-7
Appearance
Solid powder
SMILES
CNCCC(C1=CC=CC=C1)OC2=CC=C(C=C2)C(F)(F)F.Cl
InChi Key
GIYXAJPCNFJEHY-UHFFFAOYSA-N
InChi Code
InChI=1S/C17H18F3NO.ClH/c1-21-12-11-16(13-5-3-2-4-6-13)22-15-9-7-14(8-10-15)17(18,19)20;/h2-10,16,21H,11-12H2,1H3;1H
Chemical Name
N-methyl-3-phenyl-3-[4-(trifluoromethyl)phenoxy]propan-1-amine;hydrochloride
Synonyms
Lilly110140; Lilly-110140; LY-110140; Lilly 110140; Fluoxetine; Prozac; Sarafem; Fluoxetine; Animex-On; Fluoxetin; Pulvules; Eufor; Portal; LY110140; LY 110140
HS Tariff Code
2922.39.4500
Storage

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

Shipping Condition
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
Solubility Data
Solubility (In Vitro)
DMSO: ~69 mg/mL (~199.5 mM)
Water: <1 mg/mL
Ethanol: ~69 mg/mL (~199.5 mM)
Preparing Stock Solutions 1 mg 5 mg 10 mg
1 mM 2.8919 mL 14.4596 mL 28.9193 mL
5 mM 0.5784 mL 2.8919 mL 5.7839 mL
10 mM 0.2892 mL 1.4460 mL 2.8919 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.

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Working concentration mg/mL;

Method for preparing DMSO stock solution mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation:Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.

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Clinical Trial Information
NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03826875 Recruiting Drug: Fluoxetine
Drug: Placebo
Stroke Hemorrhagic
Depression
University of Washington March 1, 2019 Phase 2
NCT05634707 Recruiting Drug: Fluoxetine
Drug: Temozolomide
Primary Brain Tumor
Brain Tumor, Recurrent
Duke University August 5, 2023 Early Phase 1
NCT03228732 Recruiting Drug: Fluoxetine
Drug: Fluoxetine and DHEA
Type 1 Diabetes Mellitus University of Maryland,
Baltimore
December 19, 2017 Early Phase 1
NCT05976347 Not yet recruiting Drug: Fluoxetine 20 MG
Drug: Duloxetine 30 MG/td>
Depression Wake Forest University Health
Sciences
February 2024 Phase 4
NCT06225011 Not yet recruiting Drug: Fluoxetine Colorectal Adenocarcinoma Jonsson Comprehensive Cancer
Center
June 1, 2024 Phase 1
Biological Data
  • Chronic but not subchronic fluoxetine treatment increases cell proliferation but not the number of DCX+ immature granule cells in the dentate gyrus. J Neurosci . 2008 Feb 6;28(6):1374-84.
  • Chronic but not subchronic fluoxetine stimulates dendritic maturation of DCX+ cells. J Neurosci . 2008 Feb 6;28(6):1374-84.
  • Chronic but not subchronic fluoxetine enhances dendritic complexity of DCX+ cells. J Neurosci . 2008 Feb 6;28(6):1374-84.
  • Effects of haloperidol (1 mg/kg, s.c.) and fluoxetine (10 mg/kg, s.c.) alone and in combination on [5-HT]ex (A), [DA]ex (B), and [NE]ex (C) in the rat prefrontal cortex. Neuropsychopharmacology . 2000 Sep;23(3):250-62.
  • Effects of MDL 100907 (1 mg/kg, s.c.) and fluoxetine (10 mg/kg, s.c.) alone and in combination on [5-HT]ex (A), [DA]ex (B), and [NE]ex (C) in the rat prefrontal cortex. Neuropsychopharmacology . 2000 Sep;23(3):250-62.
  • Higher concentrations of fluoxetine have no significant impact on proliferation and cell cycle progression of MDA-MB-231 breast cancer cells. Sci Rep . 2021 Jan 13;11(1):1250.
  • Fluoxetine and sertraline marginally increase glucose uptake in SK-OV-3 cells. Sci Rep . 2021 Jan 13;11(1):1250.
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