Size | Price | Stock | Qty |
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50mg |
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100mg |
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500mg |
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Other Sizes |
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ln Vitro |
In hippocampus cells, fluoxetine prevents inevitable shock (IS) from downregulating cell proliferation [1]. Fluoxetine promotes the growth of new cells in the adult rat hippocampal dentate gyrus. In the prelimbic cortex, fluoxetine also boosts the quantity of proliferating cells [2]. Neurons in an immature state mature more quickly when taking fluoxetine. In the dentate gyrus, fluoxetine improves neurogenesis-dependent long-term potentiation (LTP) [3]. In the prefrontal cortex, fluoxetine increased extracellular levels of norepinephrine and dopamine, but not those of citalopram, fluvoxamine, paroxetine, or sertraline. Fluoxetine produces a strong and long-lasting rise in extracellular dopamine and norepinephrine concentrations after acute systemic administration [4].
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ln Vivo |
In adult male Sprague-Dawley rats exposed to inevitable shock, fluoxetine therapy also reverses the escape latency disadvantage [1]. In the dentate gyrus, fluoxetine (5 mg/kg) by itself promotes cell growth. When fluoxetine 5 mg/kg and olanzapine were administered together, there was a noteworthy rise in the quantity of BrdU-positive cells as compared to the control group [2]. Extracellular dopamine ([DA](ex)) and norepinephrine ([NE](ex)) levels increased significantly and steadily with the combination of fluoxetine and olanzapine, reaching up to 361% and 272% of baseline, respectively. when utilizing medicine alone, much greater than baseline [5].
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References |
[1]. Malberg JE, et al. Cell proliferation in adult hippocampus is decreased by inescapable stress: reversal by fluoxetine treatment. Neuropsychopharmacology. 2003 Sep;28(9):1562-71
[2]. Avitsur R1. Increased symptoms of illness following prenatal stress: Can it be prevented by fluoxetine? Behav Brain Res. 2017 Jan 15;317:62-70. [3]. Kodama M, et al. Chronic olanzapine or fluoxetine administration increases cell proliferation in hippocampus and prefrontal cortex of adult rat. Biol Psychiatry. 2004 Oct 15;56(8):570-80. [4]. Wang JW, et al. Chronic fluoxetine stimulates maturation and synaptic plasticity of adult-born hippocampal granule cells. J Neurosci. 2008 Feb 6;28(6):1374-84. [5]. Bymaster FP, et al. Fluoxetine, but not other selective serotonin uptake inhibitors, increases norepinephrine and dopamine extracellular levels in prefrontal cortex. Psychopharmacology (Berl). 2002 Apr;160(4):353-61 [6]. Zhang W, et al. Synergistic effects of olanzapine and other antipsychotic agents in combination with fluoxetine on norepinephrine and dopamine release in rat prefrontal cortex. Neuropsychopharmacology. 2000 Sep;23(3):250-62 |
Molecular Formula |
C17H18NOF3
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Molecular Weight |
309.32612
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CAS # |
54910-89-3
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Related CAS # |
Fluoxetine hydrochloride;56296-78-7;(S)-Fluoxetine hydrochloride;114247-06-2;(R)-Fluoxetine hydrochloride;114247-09-5
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SMILES |
FC(C1=CC=C(OC(C2=CC=CC=C2)CCNC)C=C1)(F)F
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : ~100 mg/mL (~323.28 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (6.72 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (6.72 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.08 mg/mL (6.72 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. Solubility in Formulation 4: 10 mg/mL (32.33 mM) in 0.5% CMC-Na/saline water (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication (<60°C). Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 3.2328 mL | 16.1640 mL | 32.3279 mL | |
5 mM | 0.6466 mL | 3.2328 mL | 6.4656 mL | |
10 mM | 0.3233 mL | 1.6164 mL | 3.2328 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.