| Size | Price | Stock | Qty |
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| 10mg |
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| 25mg |
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| 50mg |
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| 100mg |
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| 250mg |
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| Other Sizes |
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Purity: ≥98%
Dinoprost Tromethamine (Prostaglandin F2alpha; PGF2α THAM; Prostaglandin F2α THAM) is a potent, naturally occurring and orally bioactive prostaglandin that acts as a prostaglandin F (PGF) receptor (FP receptor) agonist with oxytocic, luteolytic, and abortifacient activities. It is a luteolytic hormone that is naturally produced locally in the corpus luteum (CL) and endometrial luminal epithelium. It is important for the initiation and progression of labor and is also used as a prostaglandin in medicine to induce labor.
| ln Vitro |
Dinoprost tromethamine salt (prostaglandin F2α tromethamine salt; 1 μM; for 24 hours) induces endoplasmic reticulum stress, autophagy, and apoptosis in goat luteal cells [1]. Dinoprost tromethamine salt (1 μM; for 24 hours) significantly increases the expression of GRP78 and UPR sensors [1]. Apoptosis analysis [1] Cell line: goat luteal cells Concentration: 1 μM Incubation time: 24 hours Results: Apoptosis rate increased significantly (15.62±3.12%). Autophagy detection [1] Cell line: goat luteal cells Concentration: 1 μM Incubation time: 24 hours Results: There is extensive overlap between LC3 and LAMP1 in luteal cells, and autophagic lysosomes are formed in goat luteal cells. Western Blot Analysis[1] Cell line: Goat luteal cells Concentration: 1 μM Incubation time: 24 hours Results: Expression of GRP78 and UPR sensors, including cleaved ATF6, phospho-EIF2S1, EIF2S1, ATF4, phospho-IRE1, autologous Phagocytosis-related intracellular protein LC3-II and pro-apoptotic factor cleaved Caspase3 were significantly increased.
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| References |
| Molecular Formula |
C24H45NO8
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|---|---|
| Molecular Weight |
475.6160
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| Exact Mass |
475.31
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| Elemental Analysis |
C, 60.61; H, 9.54; N, 2.94; O, 26.91
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| CAS # |
38562-01-5
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| Related CAS # |
Dinoprost; 551-11-1; Dinoprost-d4; 34210-11-2
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| Appearance |
Solid powder
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| SMILES |
CCCCC[C@@H](/C=C/[C@H]1[C@@H](C[C@@H]([C@@H]1C/C=C\CCCC(=O)O)O)O)O.C(C(CO)(CO)N)O
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| InChi Key |
IYGXEHDCSOYNKY-RZHHZEQLSA-N
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| InChi Code |
InChI=1S/C20H34O5.C4H11NO3/c1-2-3-6-9-15(21)12-13-17-16(18(22)14-19(17)23)10-7-4-5-8-11-20(24)25;5-4(1-6,2-7)3-8/h4,7,12-13,15-19,21-23H,2-3,5-6,8-11,14H2,1H3,(H,24,25);6-8H,1-3,5H2/b7-4-,13-12+;/t15-,16+,17+,18-,19+;/m0./s1
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| Chemical Name |
2-amino-2-(hydroxymethyl)propane-1,3-diol;(Z)-7-[(1R,2R,3R,5S)-3,5-dihydroxy-2-[(E,3S)-3-hydroxyoct-1-enyl]cyclopentyl]hept-5-enoic acid
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| Synonyms |
U 14585; U14585; U-14585; Dinoprost; Tromethamine; Dinoprost; Trometamol; Zinoprost; Prostamate;
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: ~95 mg/mL (~199.7 mM)
Water: ~95 mg/mL Ethanol: ~95 mg/mL |
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (5.26 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (5.26 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 2.5 mg/mL (5.26 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. Solubility in Formulation 4: 50 mg/mL (105.13 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.1025 mL | 10.5126 mL | 21.0252 mL | |
| 5 mM | 0.4205 mL | 2.1025 mL | 4.2050 mL | |
| 10 mM | 0.2103 mL | 1.0513 mL | 2.1025 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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