| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| 50mg |
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| 100mg |
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| 250mg | |||
| Other Sizes |
| Targets |
As a labeled fatty acid, Decanoic acid-d5 does not have a specific pharmacological target. Decanoic acid is a component of medium-chain triglycerides and is a non-competitive inhibitor of the AMPA receptor that permeates the blood-brain barrier. It has anti-epileptic properties. The deuterated form is used to study fatty acid metabolism and neurological effects.
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| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
Decanoic acid-d5 is used in vitro as a tracer to study fatty acid metabolism and AMPA receptor inhibition. The compound is added to cell culture media to track fatty acid uptake, incorporation into lipids, and metabolic fate. It does not exhibit intrinsic pharmacological activity beyond that of decanoic acid but serves as a quantitative tool for studying cellular lipid metabolism and neurological signaling. |
| ln Vivo |
In vivo, Decanoic acid-d5 is used in metabolic studies to trace decanoic acid absorption, distribution, metabolism, and excretion. The deuterium label allows for precise quantification of the compound and its metabolites in biological fluids and tissues using mass spectrometry. It is vital for studying the metabolic pathways, absorption, and biological transformations of fatty acids in organisms.
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| Enzyme Assay |
As an analytical standard, Decanoic acid-d5 is used in lipidomics and fatty acid analysis. Typical protocols involve spiking the labeled compound into samples prior to extraction and analysis by GC-MS or LC-MS. The compound serves as an internal standard to correct for matrix effects and instrument variability.
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| Cell Assay |
In vitro cell culture experiments using Decanoic acid-d5 involve treating cells with the labeled fatty acid and analyzing its uptake and metabolism by mass spectrometry. The compound can be used to study fatty acid metabolism, AMPA receptor inhibition, and the anti-epileptic mechanisms of decanoic acid in various cell types.
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| Animal Protocol |
In vivo animal studies using Decanoic acid-d5 involve administering the compound to rodents via oral gavage. Blood samples are collected at various time points, and the concentration of the labeled compound is measured by mass spectrometry. Tissue distribution and metabolic profiling can also be performed.
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| ADME/Pharmacokinetics |
Decanoic acid-d5 exhibits pharmacokinetic properties similar to those of unlabeled decanoic acid. As a medium-chain fatty acid, it is absorbed from the gastrointestinal tract and transported via the portal vein to the liver, where it is metabolized via beta-oxidation. It can also cross the blood-brain barrier. The deuterium label provides a distinct mass shift for analytical detection.
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| Toxicity/Toxicokinetics |
Decanoic acid-d5 is considered safe for research use at typical concentrations. Decanoic acid is a naturally occurring fatty acid with a well-established safety profile. The compound is stable if stored under recommended conditions. As a stable isotope-labeled compound, it is not intended for therapeutic use and is handled under standard laboratory safety practices.
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| References |
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| Additional Infomation |
Decanoic acid-d5 has a molecular formula of C₁₀H₁₅D₅O₂ and a molecular weight of 177.30. Purity is >98%. The compound appears as an oil and is stored at -20°C. It is primarily used as an internal standard for mass spectrometry-based quantification of decanoic acid in lipidomics and metabolic studies.
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| Molecular Formula |
C10H20O2
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|---|---|
| Molecular Weight |
177.295412063599
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| Exact Mass |
177.177
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| CAS # |
1219803-00-5
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| Related CAS # |
Decanoic acid;334-48-5
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| PubChem CID |
131869334
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| Appearance |
Colorless to off-white ointment
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| LogP |
4.1
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
2
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| Rotatable Bond Count |
8
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| Heavy Atom Count |
12
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| Complexity |
110
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| Defined Atom Stereocenter Count |
0
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| SMILES |
[2H]C([2H])([2H])C([2H])([2H])CCCCCCCC(=O)O
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| InChi Key |
GHVNFZFCNZKVNT-ZBJDZAJPSA-N
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| InChi Code |
InChI=1S/C10H20O2/c1-2-3-4-5-6-7-8-9-10(11)12/h2-9H2,1H3,(H,11,12)/i1D3,2D2
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| Chemical Name |
9,9,10,10,10-pentadeuteriodecanoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: ~200 mg/mL (~1128.0 mM; with ultrasonication)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 5 mg/mL (28.20 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (50.0 mg/mL) to 400 μL of PEG300 and mix well; then add 50 μL of Tween-80 and mix well; finally add 450 μL of physiological saline and adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 5 mg/mL (28.20 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (50.0 mg/mL) to 900 μL of 20% SBE-β-CD saline and mix well. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 5 mg/mL (28.20 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (50.0 mg/mL) to 900 μL of corn oil and mix well. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.6402 mL | 28.2008 mL | 56.4016 mL | |
| 5 mM | 1.1280 mL | 5.6402 mL | 11.2803 mL | |
| 10 mM | 0.5640 mL | 2.8201 mL | 5.6402 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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