| Size | Price | Stock | Qty |
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| 10mg |
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| 25mg |
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| 50mg |
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| 100mg |
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| 250mg |
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| 500mg |
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| 1g |
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| Other Sizes |
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Purity: ≥98%
Danshensu is a naturally occuring phenolic acid of caffeic acid derivatives isolated from Salvia miltiorrhiza, with wide cardiovascular benefit by activating Nrf2 signaling pathway. Salvia miltiorrhiza Bge contains a compound known as danshensu. Danshensu is a powerful antioxidant and radical scavenger, with superior scavenging abilities to vitamin C against free hydroxyl radicals (HO()), superoxide anion radicals (O(2)(-)), 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, and 2-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radicals.
| Targets |
CYP2E1 (IC50 = 12.6 μM); NF-κB
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| ln Vitro |
Danshensu inhibits mitochondrial membrane permeability and transmission by reducing thiol oxidation and lowers lipid peroxidation on the mitochondrial membrane by scavenging free radicals[1]. Danshensu significantly increases H9c2 cardiomyocyte cell viability while reducing lactate dehydrogenase (LDH) release. Danshensu increases Akt and extracellular signal-related kinase 1/2 (ERK1/2) phosphorylation in H9c2 cells, and the protective effects of Danshensu are only partially inhibited by the PI3K or ERK inhibitors wortmannin or U0126. Danshensu may offer significant cardioprotection against MI/R injury, and one of its possible mechanisms may involve activating the PI3K/Akt and ERK1/2 signaling pathways to prevent cardiomyocyte apoptosis. By triggering the Akt and ERK signaling pathways, Danshensu increases Bcl-2 expression while decreasing Bax and active caspase-3 expression. Danshensu has been shown to have biological effects that include increasing microcirculation, reducing the production of reactive oxygen species, inhibiting platelet adhesion and aggregation, guarding the myocardium against ischemia, and shielding endothelial cells from damage brought on by inflammation[2].
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| ln Vivo |
Comparing ISO-administered rats to rats pretreated with danshensu reveals a significant (P<0.001) reduction in ST-segment. When compared to the ISO, its pretreatment also exhibits a significant (P<0.001) decrease in serum cTnI levels. In order to protect rats' hearts from myocardial infarction caused by ISO, danshensu has significant cardioprotective effects[1]. Danshensu significantly decreases cardiac troponin (cTnI) and creatine kinase-MB (CK-MB) production in the serum and myocardium infarct size in the rat model of MI/R injury[2].
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| Cell Assay |
By substituting medium with an "ischemic buffer," which is made to mimic the extracellular environment of myocardial ischemia and contain concentrations of potassium, hydrogen, and lactate ions that are similar to those found in vivo, cardiomyocytes are subjected to ischemia. A humidified atmosphere with 5% CO2 and 95% nitrogen is used to incubate cells in the hypoxic/ischemic chamber for two hours at 37°C. Cardiomyocytes are randomly exposed to one of the following therapies at the start of reperfusion: vehicle, Danshensu (1 or 10 μM), Danshensu plus the PI3K inhibitor wortmannin (10 nM), or Danshensu plus the ERK inhibitor U0126 (10 μM). H9c2 cardiomyocytes are cultured normally in CO2 incubation for the control group's cardiomyocytes at the same time.
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| Animal Protocol |
Tolcapone is dissolved in saline that has 20% (v/v) PEG 200, whereas all other chemicals are dissolved in saline. Rats are randomly placed into different groups after being fasted for the previous night. After ethyl ether anesthesia, 200 µL of blood are alternately drawn from the orbital sinus, and the eye is then swiftly alcohol-sterilized and cotton-pressed. The instantaneous collection of blood samples into polypropylene tubes containing heparin-Na followed by a 3-minute centrifugation process at 5000 g at 5°C. The 48-hour analysis of prepared plasma samples is performed while they are kept at −20°C.
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| References |
| Molecular Formula |
C9H9O5
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| Molecular Weight |
198.17
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| Exact Mass |
198.05282342
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| CAS # |
76822-21-4
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| Related CAS # |
Danshensu sodium salt;67920-52-9;Salvianolic acid B;121521-90-2
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| Appearance |
Solid
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| SMILES |
C1=CC(=C(C=C1C[C@H](C(=O)O)O)O)O
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| InChi Key |
PAFLSMZLRSPALU-MRVPVSSYSA-N
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| InChi Code |
InChI=1S/C9H10O5/c10-6-2-1-5(3-7(6)11)4-8(12)9(13)14/h1-3,8,10-12H,4H2,(H,13,14)/t8-/m1/s1
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| Chemical Name |
(2R)-3-(3,4-dihydroxyphenyl)-2-hydroxypropanoic acid
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| Synonyms |
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
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| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: 10 mg/mL (50.46 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.0462 mL | 25.2309 mL | 50.4617 mL | |
| 5 mM | 1.0092 mL | 5.0462 mL | 10.0923 mL | |
| 10 mM | 0.5046 mL | 2.5231 mL | 5.0462 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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