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      Coelenterazine
      Coelenterazine

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      This product is for research use only, not for human use. We do not sell to patients.
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      InvivoChem Cat #: V1873
      CAS #: 55779-48-1 Purity ≥98%

      Description: Coelenterazine, a luciferin which is a light-emitting molecule, is a luminescent enzyme substrate that is used for monitoring reporter genes in BRET, ELISA and HTS techniques. It is a cell-permeable aequorin luminophore that acts as a very sensitive and specific chemiluminescent probe for the superoxide anion. Coelenterazine can also be used for detecting changes in intracellular Ca2+ in cells that have been transfected with apoaequorin cDNA. Coelenterazine also acts as a powerful antioxidant.

      References: Mol Ther. 2012 Apr;20(4):692-3. doi: 10.1038/mt.2012.38; Mol Imaging Biol. 2016 Apr;18(2):166-71.

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      Molecular Weight (MW)423.46 
      FormulaC26H21N3O3 
      CAS No.55779-48-1 
      Storage-20℃ for 3 years in powder form
      -80℃ for 2 years in solvent
      Solubility (In vitro)DMSO: 84 mg/mL (198.4 mM) 
      Water: <1 mg/mL
      Ethanol: <1 mg/mL
      SMILESC1=CC=C(C=C1)CC2=C3N=C(C(=O)N3C=C(N2)C4=CC=C(C=C4)O)CC5=CC=C(C=C5)O
      SynonymsOplophorus, luciferin; Coelenterazine; CLZN


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      In Vitro

      In vitro activity: When monitoring various biological processes, coelenterazine (CTZ) can be catalyzed by luciferases, such as the sea pansy Renilla reniformis (Rluc) and the marine copepod Gaussia princeps (Gluc), which results in emission of blue light. In Gli36-Gluc- or Gli36-Rluc-expressing Gli36 cells, coelenterazine shows a bioluminescence signal. In RIN-5F, 4T1, HCT 116, HEK 293, 293FT, HeLa and INS-1 cells, coelenterazine can be used to assay superoxide anion concentrations

      Cell Assay: Coelenterazine showed antioxidative properties in primary cultures of rat hepatocytes subjected to the oxidant tert-butyl hydroperoxide (t-BHP). Coelenterazine (3-10 μM) concentration-dependently increased survival and decreased lipid peroxidation in rat hepatocytes treated for 6 hr with t-BHP (250 μM) [2]. In addition, Coelenterazine (10 μmol/l) can be used to assay superoxide anion concentrations in cancer cell lines.

      In Vivothe small molecule coelenterazine (5 mg/kg, intravenous administration) was a potential reporter of cancer-associated superoxide anion in the 4T1 breast adenocarcinoma mouse model [1]. Gli36-Rluc or Gli36-Gluc cells were injected into the leg muscle of nude mice. The mice were injected with 4 mg/kg of coelenterazine and imaged by a cooled charge-coupled device camera. 
      Animal model5 mg/kg, intravenous administration 
      Formulation & DosageCoelenterazine (4 μg/g) formulated from an ethanol stock diluted in sodium phosphate buffer (50 mM). 
      ReferencesMol Ther. 2012 Apr;20(4):692-3. doi: 10.1038/mt.2012.38; Mol Imaging Biol. 2016 Apr;18(2):166-71. 

      These protocols are for reference only. InvivoChem does not independently validate these methods.

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