| Size | Price | Stock | Qty |
|---|---|---|---|
| 500μg |
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| 1mg |
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| 5mg |
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| 10mg |
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| Other Sizes |
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Purity: ≥99.66%
cGAMP (also known as Cyclic GMP-AMP; 3',3'-cGAMP) is a cyclic nucleotide acting as a STING agonist. It is a potent and endogenous second messenger in metazoans and triggers interferon production in response to cytosolic DNA. cGAMP induced IFNβ RNA robustly even at concentrations as low as 10 nM. cGAMP was much more potent than c-di-GMP in inducing IFNβ based on ELISA assays. cGAMP was also more potent than c-di-GMP and c-di-AMP in activating IRF3. cGAMP binds to and activates STING to trigger the downstream signaling cascades.
| ln Vitro |
The development of mouse tumor cells is stimulated by cGAMP disodium [2]. In vitro, human and mouse dendritic cells are directly activated by cGAMP disodium [2]. Patient fibroblasts exhibited enhanced transcription of IFNB1 but not of genes producing tumor factor (TNF), interleukin 6 (IL6), or interleukin 1 (IL1) when treated with cGAMP disodium [3]. The endoplasmic reticulum (ER) resident protein STING is activated by cGAMP disodium, which results in the induction of an antiviral state and type I segregin walking [4].
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| ln Vivo |
The splenocytes of immunized mice are stimulated to produce suggestive cytokines by cGAMP disodium (5 μg)—a nasal mucosal adjuvant [2].
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| Animal Protocol |
Animal/Disease Models: Female C57BL/6 (H-2b) mice 6-8 weeks old [2] Usage and
Doses: 5 µg Route of Administration: Nostril mucosal adjuvant Experimental Results: Compared with serum from OVA-immunized mice, using cGAMP adjuvant Ovalbumin (OVA)-specific IgA and total IgG as well as IgG1 and IgG2c titers were higher in the serum of OVA-immunized mice. |
| References |
[1]. Wu J, et al. Cyclic GMP-AMP is an endogenous second messenger in innate immune signaling by cytosolic DNA. Science. 2013 Feb 15;339(6121):826-30.
[2]. Skrnjug I, et al. Cyclic GMP-AMP displays mucosal adjuvant activity in mice. PLoS One. 2014 Oct 8;9(10):e110150. [3]. Ablasser A, et al. Cell intrinsic immunity spreads to bystander cells via the intercellular transfer of cGAMP. Nature. 2013 Nov 28;503(7477):530-4. |
| Molecular Formula |
C20H22N10NA2O13P2
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|---|---|
| Molecular Weight |
718.37
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| CAS # |
2407516-83-8
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| Related CAS # |
cGAMP diammonium;cGAMP;849214-04-6
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| SMILES |
O=P(O[C@H]1[C@@H](O)[C@H](N2C=NC3=C2N=CN=C3N)O[C@@H]1COP4(O)=O)(O)OC[C@@H]5[C@@H](O4)[C@@H](O)[C@H](N6C=NC7=C6N=C(N)NC7=O)O5
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| Synonyms |
Cyclic GMP-AMP
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~180 mg/mL (~250.57 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 100 mg/mL (139.20 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.3920 mL | 6.9602 mL | 13.9204 mL | |
| 5 mM | 0.2784 mL | 1.3920 mL | 2.7841 mL | |
| 10 mM | 0.1392 mL | 0.6960 mL | 1.3920 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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