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    Z-FA-FMK
    Z-FA-FMK

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    This product is for research use only, not for human use. We do not sell to patients.
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    InvivoChem Cat #: V0702
    CAS #: 197855-65-5Purity ≥98%

    Description: Z-FA-FMK, a control peptidic fluoromethylketone (boc-Thr-CH2F), is a novel and irreversible cysteine protease inhibitor, and also inhibits effector caspases. Z-FA-FMK can inhibit caspase activity in vitro and selectively inhibits recombinant effector caspases 2, -3, -6, and -7. In contrast, purified initiator caspases 8 and 10 are not affected, whereas the apoptosome-associated caspase 9 is only partially inhibited by Z-FA-FMK in vitro.

    References: Anal Biochem. 1985 Sep;149(2):461-5; J Immunol. 2006 Sep 15;177(6):3827-36.

    Related CAS#: 105637-38-5

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    Molecular Weight (MW)386.42
    FormulaC21H23N2O4F
    CAS No.197855-65-5
    Storage-20℃ for 3 years in powder form
    -80℃ for 2 years in solvent
    Solubility (In vitro)DMSO: 77 mg/mL (199.3 mM)
    Water:<1 mg/mL
    Ethanol: 34 mg/mL (88.0 mM)
    Other info

    Chemical Name: Z-FA-fluoromethyl ketone

    InChi Key: ASXVEBPEZMSPHB-YJBOKZPZSA-N

    InChi Code: InChI=1S/C21H23FN2O4/c1-15(19(25)13-22)23-20(26)18(12-16-8-4-2-5-9-16)24-21(27)28-14-17-10-6-3-7-11-17/h2-11,15,18H,12-14H2,1H3,(H,23,26)(H,24,27)/t15-,18-/m0/s1

    SMILES Code: C[[email protected]](NC(=O)[[email protected]](Cc1ccccc1)NC(=O)OCc2ccccc2)C(=O)CF

    Synonyms

    Z-FA-fluoromethyl ketone; Z-FA-FMK; 


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    In Vitro

    In vitro activity: Z-FA-FMK inhibits the degradation of fibrillar collagen by fibroblasts and osteoclasts. Z-FA-FMK inhibits LPS-induced cytokine production via inhibition of NF-kappaB-dependent gene expression in macrophages. Z-FA-FMK efficiently blocks T cell proliferation induced by mitogens and IL-2 in vitro.


    Cell Assay: T cell proliferation following mitogen stimulation is determined using [3H]thymidine incorporation. In brief, PBMCs or purified T cells are seeded in a 96-well plate and stimulated with either PHA (5 μg/ml), costimulated with anti-CD3 mAb (5 μg/ml) and anti-CD28 mAb (2.5 μg/ml) or PMA plus ionomycin in the presence or absence of z-FA-FMK. The cells are cultured for 72 h with the last 16 h pulsed with [methyl-3H]thymidine (0.037 MBq). The cells are harvested onto glass fiber filter mats using a Tomtec automated multiwell harvester. 

    In VivoZ-FA-FMK significantly increases pneumococcal growth in both lungs and blood in a mouse model of intranasal pneumococcal infection. Z-FA-FMK blocks reovirus infection of Ras oncogenic tumours and host heart tissues in severe combined immunodeficiency mice.
    Animal modelMice
    Formulation & DosageN/A
    References

    Anal Biochem. 1985 Sep;149(2):461-5; J Immunol. 2006 Sep 15;177(6):3827-36.


    These protocols are for reference only. InvivoChem does not independently validate these methods.

     

    Z-FA-FMK

    z-FA-FMK inhibits T cell proliferation and blasts formation at nontoxic concentrations. J Immunol. 2006 Sep 15;177(6):3827-36.
     

    Z-FA-FMK

    z-FA-FMK inhibits the production of IL-2 and IFN-γ in activated purified T cells. J Immunol.2006 Sep 15;177(6):3827-36.
     

    Z-FA-FMK

    The inhibition of T cell proliferation induced by z-FA-FMK is not reversed by exogenous rIL-2. J Immunol. 2006 Sep 15;177(6):3827-36.
     
    Z-FA-FMK
    The effect of z-FA-FMK on CD25 and CD69 expression in T cells following activation. J Immunol. 2006 Sep 15;177(6):3827-36.
     
    Z-FA-FMK
    z-FA-FMK inhibits IL-2-driven T cell proliferation. J Immunol. 2006 Sep 15;177(6):3827-36.
     
    Z-FA-FMK
    Effect of z-FA-FMK on DNA content in T cells following activation. J Immunol. 2006 Sep 15;177(6):3827-36.
     
    Z-FA-FMK
    The effect of z-FA-FMK on NF-κB signaling in activated T cells. J Immunol. 2006 Sep 15;177(6):3827-36.
     
    Z-FA-FMK
    z-FA-FMK blocks caspase-8 and caspase-3 activation during T cell activation. J Immunol. 2006 Sep 15;177(6):3827-36.
     
    Z-FA-FMK
    z-FA-FMK treatment increases pneumococcal load in the blood and lungs of MFI mice. J Immunol.2006 Sep 15;177(6):3827-36.


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