Size | Price | Stock | Qty |
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1mg |
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5mg |
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10mg |
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25mg |
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50mg |
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100mg |
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250mg |
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Other Sizes |
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Purity: ≥98%
Yoda 1 (GlyT2-IN-1) is a novel and potent agonist of Piezo1 which is the mechanotransduction channel. Yoda 1 activates purified Piezo1 channels. Yoda1 works by eliciting Ca2+ flux in Piezo1- but not vector-transfected cells.
ln Vitro |
Akt and ERK1/2 are activated in a Piezo1-independent manner by Yoda1 (0–6 μM, 5 min) [2]. Rac1 activation is inhibited by Yoda1 (1.5 μM, 5 min) [3].
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Cell Assay |
Western Blot analysis
Cell Types: human coronary artery endothelial cells (HCAEC) [2], A431 cells [3] Tested Concentrations: 0, 1.5, 3.0 and 6.0 μM Incubation Duration: 5 minutes Experimental Results: Induced Akt and ERK1/2 activation, and increased Phosphorylation levels of Akt and ERK1/2 were dose-dependent. Inhibits EGF-induced increase in Rac1-GTP amount and inhibits Rac1 activation |
References |
[1]. Syeda R, et al. Chemical activation of the mechanotransduction channel Piezo1. Elife. 2015 May 22;4.
[2]. Dela Paz NG, et al. Yoda1-induced phosphorylation of Akt and ERK1/2 does not require Piezo1 activation. Biochem Biophys Res Commun. 2018 Feb 26;497(1):220-225. [3]. Kuriyama M, et al. Piezo1 activation using Yoda1 inhibits macropinocytosis in A431 human epidermoid carcinoma cells. Sci Rep. 2022 Apr 15;12(1):6322. |
Molecular Formula |
C13H8CL2N4S2
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Molecular Weight |
355.265417098999
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CAS # |
448947-81-7
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Related CAS # |
448947-81-7;
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SMILES |
ClC1=C(C(Cl)=CC=C1)CSC2=NN=C(C3=NC=CN=C3)S2
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InChi Key |
BQNXBSYSQXSXPT-UHFFFAOYSA-N
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InChi Code |
InChI=1S/C13H8Cl2N4S2/c14-9-2-1-3-10(15)8(9)7-20-13-19-18-12(21-13)11-6-16-4-5-17-11/h1-6H,7H2
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Chemical Name |
2-[5-(2,6-Dichloro-benzylsulfanyl)-[1,3,4]thiadiazol-2-yl]-pyrazine
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Synonyms |
Yoda1; Yoda-1; Yoda 1
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : ~15.62 mg/mL (~43.97 mM)
Ethanol : ~5 mg/mL (~14.07 mM) |
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.56 mg/mL (4.39 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 15.6 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 2: ≥ 1.56 mg/mL (4.39 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 15.6 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 2.8148 mL | 14.0738 mL | 28.1476 mL | |
5 mM | 0.5630 mL | 2.8148 mL | 5.6295 mL | |
10 mM | 0.2815 mL | 1.4074 mL | 2.8148 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.