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    YM201636
    YM201636

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    This product is for research use only, not for human use. We do not sell to patients.
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    InvivoChem Cat #: V0137
    CAS #: 371942-69-7Purity ≥98%

    Description: YM201636, a  pyridofuropyrimidine compound, is a novel, potent and selective PIKfyve (phosphatidylinositol‑3‑phosphate 5‑kinase) inhibitor with potential anti-retroviral replication and anticancer activity,  as silencing of PIKfyve which is the sole enzyme for PtdIns(3,5)P(2) biosynthesis that controls proper endosome dynamics, can inhibit retroviral replication. YM-201636 inhibits PIKfyve with an IC50 of 33 nM. YM 201636 is able to disrupt retroviral budding at 800 nM, indicating its potential usefulness as an antiretroviral therapeutic agent [1,2]. YM201636 may also  inhibit liver tumor growth by promoting EGFR expression [3].

    References: 

    1. EMBO Rep. 2008 Feb;9(2):164-70;

    2. Biochem Biophys Res Commun. 2009 May 8;382(3):566-70;

    3. Oncol Rep. 2019 Mar;41(3):1971-1979


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    Molecular Weight (MW)

    467.48

    Formula

    C25H21N7O3

    CAS No.

    371942-69-7

    Storage

    -20℃ for 3 years in powder form

    -80℃ for 2 years in solvent

    Solubility (In vitro)

    DMSO: 35 mg/mL (74.9 mM)

    Water:<1 mg/mL

    Ethanol: <1 mg/mL

    Other info

    Synonym: YM-201636 YM201636 YM 201636.

    InChi Key: YBPIBGNBHHGLEB-UHFFFAOYSA-N

    InChi Code: InChI=1S/C25H21N7O3/c26-19-7-6-16(14-28-19)24(33)29-17-4-1-3-15(13-17)22-30-20-18-5-2-8-27-25(18)35-21(20)23(31-22)32-9-11-34-12-10-32/h1-8,13-14H,9-12H2,(H2,26,28)(H,29,33)

    SMILES Code: O=C(C1=CC=C(N)N=C1)NC2=CC=CC(C3=NC(N4CCOCC4)=C(OC5=NC=CC=C56)C6=N3)=C2           

    Chemical Name

    6-amino-N-[3-(4-morpholin-4-ylpyrido[2,3]furo[2,4-b]pyrimidin-2-yl)phenyl]pyridine-3-carboxamide

     


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    In Vitro

    In vitro: YM201636 is a selective PIKfyve inhibitor with IC50 of 33 nM, less potent to p110α and insensitive to Fabl (yeast orthologue).

     

    Kinase Assay: The assay is performed in kinase buffer (25 mM HEPES pH 7.4, 120 mM NaCl, 1.5 mM MgCl2, 5 mM 2-glycerophosphate, and 1 mM DTT) containing 100 μM PI(3)P, GST-PIKfyve, 50 μM ATP/10 μCi [32P]γATP and increasing concentrations of YM201636 in a final volume of 65 μL. The reactions are incubated for 15 minutes at 30 °C, and stopped by the addition of 243 μL of 2:1 MeOH : CHCl3 (volume : volume). Lipid products labelled with 32P are analysed by thin-layer chromatography and quantified by Cerenkov counting. IC50 value is determined by using Graphpad Prism and errors are given as ±95% confidence limit.

     

    Cell Assay: YM201636 potently inhibits mammalian PIKfyve with an IC50 of 33 nM but not yeast orthologue Fab1 with an IC50 of >5 μM, exhibiting around 100-fold selectivity for PtdIns3P p110α with an IC50 of 3 μM. YM201636 (0.8 μM) significantly decreases the production of PtdIns(3,5)P2 by 80% in serum-starved NIH3T3 cells followed by serum stimulation with no effect on serum-stimulated protein kinase B (PKB) Ser 473 phosphorylation. YM-201636 reversibly impairs endosomal trafficking in NIH3T3 cells by blocking PIKfyve and PtdIns(3,5)P2 production, mimicking the effect produced by depleting PIKfyve with siRNA. YM-201636 (0.8 μM) also significantly reduces retroviruses budding from cells by 80%, apparently through interfering with the endosomal sorting complex required for transport (ESCRT) machinery. In 3T3L1 adipocytes, YM-201636 inhibits basal and insulin-activated 2-deoxyglucose uptake with an IC50 of 54 nM, with almost complete inhibition at doses as low as 160 nM. YM-201636 (0.1 μM) has also been shown to completely block insulin-dependent activation of class IA PI 3-kinase. Although not involved in NPM-ALK-dependent proliferation and migration, YM201636 (0.4 μM) strongly reduces invasive capacities of NPM-ALK-expressing cells and their capacity to degrade the extracellular matrix. YM201636 treatment blocks the continuous recycling of junctional proteins claudin-1 and claudin-2 in MDCK cells, leading to the intracellular accumulation and delay of epithelial barrier formation.

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    References

    [1] Jefferies HB, et al. EMBO Rep, 2008, 9(2), 164-170.


    These protocols are for reference only. InvivoChem does not independently validate these methods.

    YM201636

    YM201636

    The specific inhibition of in vivo PtdIns(3,5)P2 production by YM201636. EMBO Rep, 2008, 9(2), 164-170.

    PIKfyve is the cellular target of YM201636.


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