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1mg |
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Other Sizes |
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Purity: ≥98%
YM-254890 (YM254890), a cyclic depsipeptide discovered from Chromobacterium sp. QS3666 culture broth, is a novel, potent and specific Gαq/11 inhibitor with anti-platelet activity. It can inhibit adp-induced platelet aggregation and blocking gαq/11-coupled adp receptor p2y1-mediated ca2+ mobilization. YM-254890 concentration dependently inhibited ADP-induced intracellular Ca2+ elevation, with an IC50 value of 0.92±0.28 μm. YM-254890 had no effect on the binding of fibrinogen to purified GPIIb/IIIa, but strongly inhibited binding to TRAP-stimulated washed platelets.
ln Vitro |
With IC50 values of 0.37, 0.39, and 0.51 μM, YM-254890 suppresses platelet aggregation in human platelet-rich plasma that is triggered by ADP (2, 5, and 20 μM). Platelet aggregation is mediated by ADP via the G protein-coupled receptors P2Y1 and P2Y12. C6-15 cells that transiently expressed human P2Y1 or P2Y12 receptors were used to investigate the effects of YM-254890 on the P2Y1 and P2Y12 signaling pathways. When 2MeSADP is used to stimulate P2Y1-C6-15 cells, there is a greater intracellular calcium mobilization. With an IC50 value of 0.031 μM, YM-254890 suppresses the rise in [Ca2+]i in this experiment. On the other hand, in P2Y12-C6-15 cells, YM-254890 at 40 μM had no effect on the 2MeSADP-induced suppression of forskolin-stimulated adenylyl cyclase activity [1].
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References |
[1]. Taniguchi M, et al. YM-254890, a novel platelet aggregation inhibitor produced by Chromobacterium sp. QS3666. J Antibiot (Tokyo). 2003 Apr;56(4):358-63.
[2]. Zhang H, et al. Structure-activity relationship and conformational studies of the natural product cyclic depsipeptides YM-254890 and FR900359. Eur J Med Chem. 2018 Aug 5;156:847-860. |
Molecular Formula |
C46H69N7O15
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Molecular Weight |
960.077973127365
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CAS # |
568580-02-9
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Related CAS # |
568580-02-9 (YM-254890); 1702378-78-6 ( WU-07047, a simplified analog of the selective Gαq/11 inhibitor YM-25489) |
SMILES |
CC(C)[C@@H](O)[C@H](NC(C)=O)C(O[C@@H]([C@H](NC([C@H](C)N(C)C([C@H](C)NC(C(N(C)C([C@@H](CC1=CC=CC=C1)O2)=O)=C)=O)=O)=O)C(N(C)[C@@H]([C@H](OC)C)C(O[C@H](C)[C@H](NC(C)=O)C2=O)=O)=O)C(C)C)=O
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InChi Key |
QVYLWCAYZGFGNF-WBWCVGBTSA-N
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InChi Code |
InChI=1S/C46H69N7O15/c1-22(2)37(56)34(49-30(11)55)45(63)68-38(23(3)4)35-43(61)53(14)36(28(9)65-15)46(64)66-27(8)33(48-29(10)54)44(62)67-32(21-31-19-17-16-18-20-31)42(60)52(13)25(6)39(57)47-24(5)41(59)51(12)26(7)40(58)50-35/h16-20,22-24,26-28,32-38,56H,6,21H2,1-5,7-15H3,(H,47,57)(H,48,54)(H,49,55)(H,50,58)/t24-,26-,27+,28+,32+,33-,34-,35-,36-,37+,38+/m0/s1
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Chemical Name |
(R)-1-((3S,6S,9S,12S,18R,21S,22R)-21-Acetamido-18-benzyl-3-((R)-1-methoxyethyl)-4,9,10,12,16,22-hexamethyl-15-methylene-2,5,8,11,14,17,20-heptaoxo-1,19-dioxa-4,7,10,13,16-pentaazacyclodocosan-6-yl)-2-methylpropyl (2S,3R)-2-acetamido-3-hydroxy-4-methylpentanoate
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Synonyms |
YM254890; YM 254890; YM-254890
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : ~50 mg/mL (~52.08 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.25 mg/mL (1.30 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1.25 mg/mL (1.30 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 1.25 mg/mL (1.30 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), suspension solution. |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 1.0416 mL | 5.2079 mL | 10.4158 mL | |
5 mM | 0.2083 mL | 1.0416 mL | 2.0832 mL | |
10 mM | 0.1042 mL | 0.5208 mL | 1.0416 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Effects of YM-254890 (a) and MRS2179 (b) on ADP-induced human platelet intracellular Ca2+elevation.Br J Pharmacol. 2006 May; 148(1): 61–69. th> |
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Effects of YM-254890 and MRS2179 on P-selectin expression and fibrinogen binding.Br J Pharmacol. 2006 May; 148(1): 61–69. td> |
Effect of YM-254890 on human platelet shape change after the addition of various agonists. Effect of YM-254890 on SIPA in human PRP.Br J Pharmacol. 2006 May; 148(1): 61–69. td> |