| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 25mg |
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| 50mg |
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WYC-209, a synthetic retinoid, is a retinoic acid receptor (RAR) agonist. WYC-209 induces apoptosis primarily via the caspase 3 pathway (IC50=0.19 μM for inmalignant murine melanoma TRCs), and has long-term effects with little toxicity.
| ln Vitro |
Long-lasting effects are observed in the inhibition and blocking of human tumor cell TRC growth in culture by WYC-209 (10 μM; 24 hours) [1].
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|---|---|
| ln Vivo |
In C57BL/6 mice with lung metastases, WYC-209 (0.022-0.22 mg/kg; intravenous injection; once every two days for 25 days) suppresses tumor metastasis [1].
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| Cell Assay |
Apoptosis analysis [1]
Cell Types: A2780, A549, MCF-7, MDA-MB-435s, A375 Cell Tested Concentrations: 10 μM Incubation Duration: 24 hrs (hours) Experimental Results: TRC failed to resume growth 5 days after drug elution. |
| Animal Protocol |
Animal/Disease Models: Female immunocompetent C57BL/6 mice [1]
Doses: 0.022, 0.22 mg/kg Route of Administration: intravenous (iv) (iv)injection; once every two days for 25 days Experimental Results: At the dose of 0.022 mg/kg, 8 mice Four of the mice developed lung metastases, and at the dose of 0.22 mg/kg, only one of the eight mice developed lung metastases. |
| References |
[1]. Chen J, et al. Inhibition of cancer stem cell like cells by a synthetic retinoid. Nat Commun. 2018 Apr 11;9(1):1406.
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| Molecular Formula |
C22H22O3S
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|---|---|
| Molecular Weight |
366.475
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| Exact Mass |
366.129
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| CAS # |
2131803-90-0
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| Related CAS # |
WYC-210;2131803-93-3
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| SMILES |
O=C(OCC)C1=CC=C(C#CC2=CC(C(C)(C)CCS3=O)=C3C=C2)C=C1
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| InChi Key |
BTLUWTPBBDYOKU-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C22H22O3S/c1-4-25-21(23)18-10-7-16(8-11-18)5-6-17-9-12-20-19(15-17)22(2,3)13-14-26(20)24/h7-12,15H,4,13-14H2,1-3H3
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| Chemical Name |
C22H22O3S
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| Synonyms |
WYC-209 WYC 209 WYC209
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~83.33 mg/mL (~226.16 mM)
H2O : < 0.1 mg/mL |
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (5.65 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (5.65 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.7287 mL | 13.6433 mL | 27.2866 mL | |
| 5 mM | 0.5457 mL | 2.7287 mL | 5.4573 mL | |
| 10 mM | 0.2729 mL | 1.3643 mL | 2.7287 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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