Triamcinolone Acetonide

Alias:
Cat No.:V1710 Purity: ≥98%
Triamcinolone acetonide (Acetospan; Adcortyl A; AllerNaze;Kenalone; Nasacort; Aristocort; Aristoderm.
Triamcinolone Acetonide Chemical Structure CAS No.: 76-25-5
Product category: Calcium Channel
This product is for research use only, not for human use. We do not sell to patients.
Size Price Stock Qty
1g
2g
5g
10g
Other Sizes

Other Forms of Triamcinolone Acetonide:

  • Triamcinolone acetonide-d6
Official Supplier of:
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Purity & Quality Control Documentation

Purity: ≥98%

Product Description

Triamcinolone acetonide (Acetospan; Adcortyl A; AllerNaze; Kenalone; Nasacort; Aristocort; Aristoderm. Aristogel) is a potent and synthetic glucocorticoid with improved anti-inflammatory activities.

Biological Activity I Assay Protocols (From Reference)
ln Vitro
As the concentration rises, triamcinolone acetonide (0.05-3 mg/mL, 48-60 h) decreases BRECs' capacity to proliferate [1]. In a concentration-dependent manner, triamcinolone acetonide (0.04-5 mg/mL, 24 h) decreases the viability of both normal and osteoarthritic (OA) chondrocytes [2]. The degree of cartilage structural degradation, chondrocyte loss and colony formation, and proteoglycan loss in OA cartilage are all made worse by triamcinolone acetonide (0.04-5 mg/mL, 24 h) [2]. Strongly inducing monocyte differentiation toward M2 and anti-inflammatory macrophage phenotypes is triamcinolone acetonide (100 nM, 7 days) [3].
ln Vivo
In rats with osteoarthritis, intraperitoneal injection of 1.43 mg/mL triamcinolone acetonide once a week for 6–12 weeks totally stops the development of osteophytes and improves FRβ-related macrophage activation [3].
Cell Assay
Cell Viability Assay[2]
Cell Types: Chondrocyte
Tested Concentrations: 0.04, 0.08, 0.16, 0.31, 0.63, 1.25, 2.5, and 5 mg/ml
Incubation Duration: 24 h
Experimental Results: decreased cell viability with the value of IC50 was 2.23 mg/ mL in normal chondrocytes and 1.14 mg/mL in OA chondrocytes.
Animal Protocol
Animal/Disease Models: Severe OA rat model [3]
Doses: 1.43 mg/mL
Route of Administration: intraperitoneal (ip)injection
Experimental Results: diminished body weight during OA induction. demonstrated more macrophage activation and minimal or no osteophyte formation when injected knee joints.
References
[1]. Spandau U H M, et al. Effect of triamcinolone acetonide on proliferation of retinal endothelial cells in vitro and in vivo [J]. British journal of ophthalmology, 2005, 89(6): 745-747.
[2]. Euppayo T, et al. In vitro effects of triamcinolone acetonide and in combination with hyaluronan on canine normal and spontaneous osteoarthritis articular cartilage [J]. In Vitro Cellular & Developmental Biology-Animal, 2016, 52: 723-735.
[3]. Siebelt M, et al. Triamcinolone acetonide activates an anti-inflammatory and folate receptor–positive macrophage that prevents osteophytosis in vivo [J]. Arthritis research & therapy, 2015, 17(1): 1-13.
[4]. Jensen J M, et al. Effects of pimecrolimus compared with triamcinolone acetonide cream on skin barrier structure in atopic dermatitis: a randomized, double-blind, right–left arm trial [J]. Acta Dermato-Venereologica, 2013, 93(5): 515-519.
[5]. http://en.wikipedia.org/wiki/Triamcinolone_acetonide
[6]. Zhen Xiang, et al. Glucocorticoids improve severe or critical COVID-19 by activating ACE2 and reducing IL-6 levels. Int J Biol Sci 2020; 16(13):2382-2391.
These protocols are for reference only. InvivoChem does not independently validate these methods.
Physicochemical Properties
Molecular Formula
C24H31FO6
Molecular Weight
434.5
CAS #
76-25-5
SMILES
F[C@@]12[C@]3(C([H])=C([H])C(C([H])=C3C([H])([H])C([H])([H])[C@@]1([H])[C@]1([H])C([H])([H])[C@]3([H])[C@](C(C([H])([H])O[H])=O)([C@@]1(C([H])([H])[H])C([H])([H])[C@]2([H])O[H])OC(C([H])([H])[H])(C([H])([H])[H])O3)=O)C([H])([H])[H]
InChi Key
YNDXUCZADRHECN-JNQJZLCISA-N
InChi Code
InChI=1S/C24H31FO6/c1-20(2)30-19-10-16-15-6-5-13-9-14(27)7-8-21(13,3)23(15,25)17(28)11-22(16,4)24(19,31-20)18(29)12-26/h7-9,15-17,19,26,28H,5-6,10-12H2,1-4H3/t15-,16-,17-,19+,21-,22-,23-,24+/m0/s1
Chemical Name
(6aS,6bR,7S,8aS,8bS,11aR,12aS,12bS)-6b-fluoro-7-hydroxy-8b-(2-hydroxyacetyl)-6a,8a,10,10-tetramethyl-1,2,6a,6b,7,8,8a,8b,11a,12,12a,12b-dodecahydro-4H-naphtho[2,1:4,5]indeno[1,2-d][1,3]dioxol-4-one
Synonyms

Acetospan; Adcortyl A; AllerNaze;Kenalone; Nasacort; Aristocort. Aristocort A. Aristocort acetonide. Aristoderm. Aristogel.

Storage

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

Shipping Condition
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
Solubility Data
Solubility (In Vitro)
DMSO:87 mg/mL (200.2 mM)
Water:<1 mg/mL
Ethanol:13 mg/mL (29.9 mM)
Preparing Stock Solutions 1 mg 5 mg 10 mg
1 mM 2.3015 mL 11.5075 mL 23.0150 mL
5 mM 0.4603 mL 2.3015 mL 4.6030 mL
10 mM 0.2301 mL 1.1507 mL 2.3015 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.

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Method for preparing DMSO stock solution mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation:Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.

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