| Size | Price | Stock | Qty |
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| 100mg |
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Purity: ≥98%
Tetrandrine (also called Fanchinine; Sinomenine A; d-tetrandrine; formerly NSC-77037), a bis-benzylisoquinoline alkaloid derived from Stephania tetrandra, is a novel and potent calcium channel blocker which inhibits voltage-gated Ca2+ current (ICa) and Ca2+-activated K+ current. Tetrandrine inhibits the L-type calcium channels (IC50 = 0.3-8 µM) and T-type calcium channels (IC50 = 2.5-20 µM). Moreover, the Ca2+-activated K+ channel (Kd=0.2 µM) is strongly blocked by it. In isolated nerve terminals of the rat neurohypophysis, tetrandrine inhibits voltage-gated Ca2+ currents with an IC50 of 10.1 mM. With an IC50 of 0.21 mM, tetrandrine is a high-affinity blocker of the type II, maxi-Ca(2+)-activated K+ channel of the rat neurohypophysial terminals.
| Targets |
Ca2+ current; K+ current
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| Cell Assay |
In a 96-well plate, Huh7, HCCLM9, and Hep3B cells are seeded at a density of 5 × 103 cells/well. For twenty-four hours, the cells are exposed to Tetrandrine (NSC-77037) at the indicated concentrations (0–4 μM). After staining the cells for one to two hours with 20 μL of MTS, the plates are read at 490 nm using a BioTek ELx800[2].
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| Animal Protocol |
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| References |
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| Molecular Formula |
C38H42N2O6
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| Molecular Weight |
622.75
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| Exact Mass |
622.3
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| Elemental Analysis |
C, 73.29; H, 6.80; N, 4.50; O, 15.41
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| CAS # |
518-34-3
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| Related CAS # |
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| Appearance |
Solid powder
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| SMILES |
CN1CCC2=CC(=C3C=C2[C@@H]1CC4=CC=C(C=C4)OC5=C(C=CC(=C5)C[C@H]6C7=C(O3)C(=C(C=C7CCN6C)OC)OC)OC)OC
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| InChi Key |
WVTKBKWTSCPRNU-KYJUHHDHSA-N
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| InChi Code |
InChI=1S/C38H42N2O6/c1-39-15-13-25-20-32(42-4)34-22-28(25)29(39)17-23-7-10-27(11-8-23)45-33-19-24(9-12-31(33)41-3)18-30-36-26(14-16-40(30)2)21-35(43-5)37(44-6)38(36)46-34/h7-12,19-22,29-30H,13-18H2,1-6H3/t29-,30-/m0/s1
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| Chemical Name |
(1S,14S)-9,20,21,25-tetramethoxy-15,30-dimethyl-7,23-dioxa-15,30-diazaheptacyclo[22.6.2.23,6.18,12.114,18.027,31.022,33]hexatriaconta-3(36),4,6(35),8,10,12(34),18,20,22(33),24,26,31-dodecaene
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| Synonyms |
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
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| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 0.5 mg/mL (0.80 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 5.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 0.5 mg/mL (0.80 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 5.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 0.5 mg/mL (0.80 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. Solubility in Formulation 4: 10 mg/mL (16.06 mM) in 50% PEG300 50% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.6058 mL | 8.0289 mL | 16.0578 mL | |
| 5 mM | 0.3212 mL | 1.6058 mL | 3.2116 mL | |
| 10 mM | 0.1606 mL | 0.8029 mL | 1.6058 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
| NCT Number | Recruitment | interventions | Conditions | Sponsor/Collaborators | Start Date | Phases |
| NCT05697029 | Not yet recruiting | Drug: Tetrandrine | COVID-19 | Peking University Third Hospital |
December 31, 2023 | Phase 4 |
| NCT05245448 | Not yet recruiting | Drug: Tetrandrine Drug: Placebo |
Rheumatoid Arthritis | Peking University People's Hospital |
February 22, 2022 | Not Applicable |
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