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Purity: ≥98%
Tetracycline (NSC-108579, Sumycin among others) is a potent and broad-spectrum antibiotic used to treat a number of infections such as gram-positive and gram-negative bacteria. This includes acne, cholera, brucellosis, plague, malaria, and syphilis. It possesses some level of bacteriostatic activity against almost all medically relevant aerobic and anaerobic bacterial genera, both Gram-positive and Gram-negative.
Targets |
Tetracycline
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ln Vitro |
With a MIC value of 0.5 g/mL, tetracycline demonstrates susceptibility to V. vulnificus strain B3547[2].
Tetracycline breaks down pre-formed fibrils and prevents the formation of L-amyloid aggregates[3]. |
ln Vivo |
Mice with bacteremia are rescued from death by tetracycline (3 mg/kg; i.p. every 12 h until survive)[2].
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Animal Protocol |
Animal Model: 30 to 40 g female V. vulnificus strain B3547 infected ICR mice[2]
Dosage: 3 mg/kg
Administration: Intraperitoneal injection; 3 mg/kg every 12h until survive
Result: prevented human CNE-2 xenografts from growing in nude mice.
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References |
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Molecular Formula |
C22H4N2O8
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Molecular Weight |
444.4346
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Exact Mass |
444.15
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Elemental Analysis |
C, 59.46; H, 5.44; N, 6.30; O, 28.80
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CAS # |
60-54-8
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Related CAS # |
Tetracycline-d6;2373374-42-4;Tetracycline hydrochloride;64-75-5
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Appearance |
Solid powder
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SMILES |
O=C(C(C1=O)=C(O)[C@@H](N(C)C)[C@]2([H])C[C@]3([H])[C@](C)(O)C4=C(C(C3=C(O)[C@@]21O)=O)C(O)=CC=C4)N
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InChi Key |
OFVLGDICTFRJMM-WESIUVDSSA-N
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InChi Code |
InChI=1S/C22H24N2O8/c1-21(31)8-5-4-6-11(25)12(8)16(26)13-9(21)7-10-15(24(2)3)17(27)14(20(23)30)19(29)22(10,32)18(13)28/h4-6,9-10,15,25,27-28,31-32H,7H2,1-3H3,(H2,23,30)/t9-,10-,15-,21+,22-/m0/s1
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Chemical Name |
2-Naphthacenecarboxamide,
4-(dimethylamino)-1,4,4a,5,5a,6,11,12a-octahydro-3,6,10,12,12a-pentahydroxy-6-methyl-1,11-dioxo-,
(4S,4aS,5aS,6S,12aS)-
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Synonyms |
Mericycline; Micycline; Neocycline; NSC 108579; NSC-108579; NSC108579
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product is not stable in solution, please use freshly prepared working solution for optimal results. |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : 89~125 mg/mL (200.25~281.26 mM)
Ethanol : ~22 mg/mL H2O : < 0.1 mg/mL |
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (4.68 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (4.68 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 2.2501 mL | 11.2504 mL | 22.5007 mL | |
5 mM | 0.4500 mL | 2.2501 mL | 4.5001 mL | |
10 mM | 0.2250 mL | 1.1250 mL | 2.2501 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Structure of 6-deoxy-6-demethyltetracycline, the minimum tetracycline pharmacophore.[1]. Tetracycline antibiotics: mode of action, applications, molecular biology, and epidemiology ofbacterial resistance. Microbiol Mol Biol Rev. 2001 Jun;65(2):232-60. td> |
Stereochemical and substitution requirements for optimum antibacterial activity within the tetracycline series.[1]. Tetracycline antibiotics: mode of action, applications, molecular biology, and epidemiology ofbacterial resistance. Microbiol Mol Biol Rev. 2001 Jun;65(2):232-60. td> |