Tamoxifen

Cat No.:V1742 Purity: ≥98%
Tamoxifen (ICI-46474; NSC-180973; Nolvadex; Novaldex) is a potent and selective estrogen receptor modulator(SERM) with potential antitumor activity.
Tamoxifen Chemical Structure CAS No.: 10540-29-1
Product category: Autophagy
This product is for research use only, not for human use. We do not sell to patients.
Size Price Stock Qty
500mg
1g
2g
5g
10g
25g
Other Sizes

Other Forms of Tamoxifen:

  • Tamoxifen Citrate
  • Tamoxifen-d5
  • Tamoxifen-d3 (tamoxifen-d3; ICI 47699-d3; (Z)-Tamoxifen-d3; trans-Tamoxifen-d3)
Official Supplier of:
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Purity & Quality Control Documentation

Purity: ≥98%

Purity: ≥98%

Product Description

Tamoxifen (ICI-46474; NSC-180973; Nolvadex; Novaldex) is a potent and selective estrogen receptor modulator (SERM) with potential antitumor activity. It acts by competitively inhibiting estrogen binding to the ER receptors in breast tissues. In other tissues (e.g. endometrium), Tamoxifen acts as an agonist, and thus may be characterized as a selective estrogen-receptor modulator. Tamoxifen is the usual endocrine (anti-estrogen) therapy for hormone receptor-positive breast cancer in pre-menopausal women, and is also a standard in post-menopausal women although aromatase inhibitors are also frequently used in that setting [1,2]. Tamoxifen can induce gene knockout of CreER(T2) transgenic mouse [3].

Biological Activity I Assay Protocols (From Reference)
ln Vitro
Tamoxifen (ICI 47699) does not influence MDA-MB-231 cells, but it has a significant inhibitory effect on MCF-7 cells (EC50=1.41 μM) and a lessened inhibitory effect on T47D cells (EC50=2.5 μM) [2].
ln Vivo
Gene knockout occurs when premutation mice receive an injection of tamoxifen (75 mg/kg; administered every five days at 6 weeks of age) which causes floxed exon excision [3].
Animal Protocol
Animal/Disease Models: Aldh1l1-cre/ERT2 x Ai95 mice[3]
Doses: 75 mg/kg
Route of Administration: Injected for 5 days at 6 weeks of age
Experimental Results: Resulted in the excision of the floxed exon and a gene knockout.
References
[1]. Osborne CK. Tamoxifen in the treatment of breast cancer. N Engl J Med. 1998 Nov 26;339(22):1609-18.
[2]. Hawariah A, et al. In vitro response of human breast cancer cell lines to the growth-inhibitory effects of styrylpyrone derivative (SPD) and assessment of its antiestrogenicity. Anticancer Res. 1998 Nov-Dec;18(6A):4383-6.
[3]. Jun Nagai, et al. Hyperactivity with Disrupted Attention by Activation of an Astrocyte Synaptogenic Cue. Cell. 2019 May 16;177(5):1280-1292.e20.
[4]. Zhao R, et al. Tamoxifen enhances the Hsp90 molecular chaperone ATPase activity. PLoS One. 2010 Apr 1;5(4):e9934.
[5]. Kedjouar B, et al. Molecular characterization of the microsomal tamoxifen binding site. J Biol Chem. 2004 Aug 6;279(32):34048-61.
[6]. Feil S, et, al. Inducible Cre mice. Methods Mol Biol. 2009;530:343-63.
[7]. Laura Cooper, et al. Screening and Reverse-Engineering of Estrogen Receptor Ligands as Potent Pan-Filovirus Inhibitors. J Med Chem. 2020 Sep 4.
These protocols are for reference only. InvivoChem does not independently validate these methods.
Physicochemical Properties
Molecular Formula
C26H29NO
Molecular Weight
371.51
CAS #
10540-29-1
SMILES
O(C([H])([H])C([H])([H])N(C([H])([H])[H])C([H])([H])[H])C1C([H])=C([H])C(=C([H])C=1[H])/C(/C1C([H])=C([H])C([H])=C([H])C=1[H])=C(\C1C([H])=C([H])C([H])=C([H])C=1[H])/C([H])([H])C([H])([H])[H]
Storage

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

Shipping Condition
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
Solubility Data
Solubility (In Vitro)
DMSO: 74 mg/mL (199.2 mM)
Water:<1 mg/mL
Ethanol:74 mg/mL (199.2 mM)
Solubility (In Vivo)

Corn oil:40 mg/mL or 107.67 mM

 (Please use freshly prepared in vivo formulations for optimal results.)
Preparing Stock Solutions 1 mg 5 mg 10 mg
1 mM 2.6917 mL 13.4586 mL 26.9172 mL
5 mM 0.5383 mL 2.6917 mL 5.3834 mL
10 mM 0.2692 mL 1.3459 mL 2.6917 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.

Calculator

Molarity Calculator allows you to calculate the mass, volume, and/or concentration required for a solution, as detailed below:

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An example of molarity calculation using the molarity calculator is shown below:
What is the mass of compound required to make a 10 mM stock solution in 5 ml of DMSO given that the molecular weight of the compound is 350.26 g/mol?
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Dilution Calculator allows you to calculate how to dilute a stock solution of known concentrations. For example, you may Enter C1, C2 & V2 to calculate V1, as detailed below:

What volume of a given 10 mM stock solution is required to make 25 ml of a 25 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=25 μM, V2=25 ml and V1 is the unknown:
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  • The answer of 62.5 μL (0.1 ml) appears in the Volume (Start) box
g/mol

Molecular Weight Calculator allows you to calculate the molar mass and elemental composition of a compound, as detailed below:

Note: Chemical formula is case sensitive: C12H18N3O4  c12h18n3o4
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Definitions of molecular mass, molecular weight, molar mass and molar weight:
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  • Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.
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In vivo Formulation Calculator (Clear solution)
Step 1: Enter information below (Recommended: An additional animal to make allowance for loss during the experiment)
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Calculation results

Working concentration mg/mL;

Method for preparing DMSO stock solution mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation:Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
             (2) Be sure to add the solvent(s) in order.

Biological Data
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