Tamoxifen Citrate

Alias: ICI 46474 Citrate;NSC-180973,ICI 46474, NSC180973,ICI-46474, ICI46474, NSC 180973, tamoxifen, tamoxifeni citras, Nolvadex, Novaldex
Cat No.:V1722 Purity: ≥98%
Tamoxifen Citrate (ICI-46474, NSC-180973; Nolvadex, Novaldex), the citrate salt of ICI-46474, is a selective estrogen receptor modulator(SERM) with potent antitumor activity.
Tamoxifen Citrate Chemical Structure CAS No.: 54965-24-1
Product category: Autophagy
This product is for research use only, not for human use. We do not sell to patients.
Size Price Stock Qty
500mg
1g
2g
5g
Other Sizes

Other Forms of Tamoxifen Citrate:

  • Tamoxifen
  • Tamoxifen-d5
Official Supplier of:
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Purity & Quality Control Documentation

Purity: ≥98%

Product Description

Tamoxifen Citrate (ICI-46474, NSC-180973; Nolvadex, Novaldex), the citrate salt of ICI-46474, is a selective estrogen receptor modulator (SERM) with potent antitumor activity. It acts by competitively inhibiting estrogen binding in breast tissues. In other tissues like the endometrium, tamoxifen acts as an agonist, and thus is is called a selective estrogen-receptor modulator. Tamoxifen is has been approved for treating hormone receptor-positive breast cancer in pre-menopausal women.

Biological Activity I Assay Protocols (From Reference)
ln Vitro
Tamoxifen Citrate (ICI 46474) does not influence MDA-MB-231 cells, but it has a significant inhibitory effect on MCF-7 cells (EC50=1.41 μM) and a lessened inhibitory effect on T47D cells (EC50=2.5 μM)[2].
ln Vivo
The ability to arbitrarily eliminate gene expression in adult animals in a tissue-specific way is one of the strategy's obvious advantages when using Tamoxifen Citrate-induced knockout. Tamoxifen citrate, 65 mg/kg per day, was intraperitoneally given into 7-week-old TmcsMed1-/- mice to investigate the function of Med1 in the adult heart. The mice were then euthanized at predetermined intervals. time after that. Three days after the Tamoxifen Citrate injection, Med1 expression started to decline (by about 70%), and five days later, Med1 expression in the heart was essentially nonexistent, according to qPCR analysis of RNA. Dilated cardiomyopathy is the result of heart-specific disruption of Med1 (TmcsMed1-/-) in adult mice caused by tamoxifen citrate [3].
Animal Protocol
Formulated in Silastic capsules; 2 cm Tamoxifen capsules; s.c. implantation
Human breast carcinoma xenografts MCF-7
References
[1]. Osborne CK. Tamoxifen in the treatment of breast cancer. N Engl J Med. 1998 Nov 26;339(22):1609-18.
[2]. Hawariah A, et al. In vitro response of human breast cancer cell lines to the growth-inhibitory effects of styrylpyrone derivative (SPD) and assessment of its antiestrogenicity. Anticancer Res. 1998 Nov-Dec;18(6A):4383-6.
[3]. Jia Y, et al. Cardiomyocyte-Specific Ablation of Med1 Subunit of the Mediator Complex Causes Lethal DilatedCardiomyopathy in Mice. PLoS One. 2016 Aug 22;11(8):e0160755.
[4]. Zhao R, et al. Tamoxifen enhances the Hsp90 molecular chaperone ATPase activity. PLoS One. 2010 Apr 1;5(4):e9934.
[5]. Laura Cooper, et al. Screening and Reverse-Engineering of Estrogen Receptor Ligands as Potent Pan-Filovirus Inhibitors. J Med Chem. 2020 Sep 4.
[6]. Feil S, et, al. Inducible Cre mice. Methods Mol Biol. 2009;530:343-63
These protocols are for reference only. InvivoChem does not independently validate these methods.
Physicochemical Properties
Molecular Formula
C26H29NO.C6H8O7
Molecular Weight
563.64
CAS #
54965-24-1
SMILES
O(C([H])([H])C([H])([H])N(C([H])([H])[H])C([H])([H])[H])C1C([H])=C([H])C(=C([H])C=1[H])/C(/C1C([H])=C([H])C([H])=C([H])C=1[H])=C(\C1C([H])=C([H])C([H])=C([H])C=1[H])/C([H])([H])C([H])([H])[H].O([H])C(C(=O)O[H])(C([H])([H])C(=O)O[H])C([H])([H])C(=O)O[H]
Synonyms
ICI 46474 Citrate;NSC-180973,ICI 46474, NSC180973,ICI-46474, ICI46474, NSC 180973, tamoxifen, tamoxifeni citras, Nolvadex, Novaldex
Storage

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

Shipping Condition
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
Solubility Data
Solubility (In Vitro)
DMSO: 100 mg/mL (177.4 mM)
Water:<1 mg/mL
Ethanol:<1 mg/mL
Solubility (In Vivo)
30% PEG400+0.5% Tween80+5%Propylene glycol: 30mg/mL
 (Please use freshly prepared in vivo formulations for optimal results.)
Preparing Stock Solutions 1 mg 5 mg 10 mg
1 mM 1.7742 mL 8.8709 mL 17.7418 mL
5 mM 0.3548 mL 1.7742 mL 3.5484 mL
10 mM 0.1774 mL 0.8871 mL 1.7742 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.

Calculator

Molarity Calculator allows you to calculate the mass, volume, and/or concentration required for a solution, as detailed below:

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An example of molarity calculation using the molarity calculator is shown below:
What is the mass of compound required to make a 10 mM stock solution in 5 ml of DMSO given that the molecular weight of the compound is 350.26 g/mol?
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Dilution Calculator allows you to calculate how to dilute a stock solution of known concentrations. For example, you may Enter C1, C2 & V2 to calculate V1, as detailed below:

What volume of a given 10 mM stock solution is required to make 25 ml of a 25 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=25 μM, V2=25 ml and V1 is the unknown:
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g/mol

Molecular Weight Calculator allows you to calculate the molar mass and elemental composition of a compound, as detailed below:

Note: Chemical formula is case sensitive: C12H18N3O4  c12h18n3o4
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Definitions of molecular mass, molecular weight, molar mass and molar weight:
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In vivo Formulation Calculator (Clear solution)
Step 1: Enter information below (Recommended: An additional animal to make allowance for loss during the experiment)
Step 2: Enter in vivo formulation (This is only a calculator, not the exact formulation for a specific product. Please contact us first if there is no in vivo formulation in the solubility section.)
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Calculation results

Working concentration mg/mL;

Method for preparing DMSO stock solution mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation:Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
             (2) Be sure to add the solvent(s) in order.

Biological Data
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