Streptozotocin (STZ)

Alias: NSC-85998; U9889; AI3-50821; NRRL 2697; U-9889; U 9889; NCI-C03167; NSC-85998; STZ; SZC; SZN; Zanosar

Cat No.:V1856 Purity: =98.22%

COA Product Data Instructions for use SDS

Streptozotocin (STZ) Chemical Structure CAS No.: 18883-66-4
Product category: DNA(RNA) Synthesis

This product is for research use only, not for human use. We do not sell to patients.

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Nature 2021, 597(7874):119-125.

Cell 2020,183:1202-1218.e25.

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Cell 2020,183:1202-1218.e25.

Science Adv 2022:8,eabm9427.

Nature 2021,597(7874):119-125.

Cell 2020,183:1202-1218.e25.

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InvivoChem's Streptozotocin (STZ) has been cited by 2 publications

Purity & Quality Control Documentation

Current batch：

Purity: =98.22%

COA

MSDS

NMR

HPLC

Instructions

Product Description
Bioactivity & Protocols
Physicochemical Properties
Solubility Data
Calculators
Clinical Trial Information
Biological Data

Product Description

Streptozotocin (also known as NSC-85998, STZ, Streptozotocin; U 9889) is a glucosamine-nitrosourea derivative and a potent DNA-methylating agent with IC50s of 11.7, 904 and 1024 μg/mL in HL60, K562 and C1498 cells respectively. Streptozotocin is an antibiotic, methylating, carcinogen, and inducer of diabetes. Antineoplastic antibiotic streptozocin is methylnitrosourea that was extracted from the Streptomyces achromogenes bacteria. DNA is alkylated by streptozocin, which also prevents DNA synthesis and creates interstrand cross-links. This agent is easily absorbed by pancreatic beta cells because of its glucose moiety, which causes diabetes mellitus at high concentrations.

Biological Activity I Assay Protocols (From Reference)

Targets

DNA alkylator

ln Vitro

Streptozotocin is a highly genotoxic agent that directly methylates DNA. It can cause chromosomal aberrations, micronuclei, sister chromatid exchanges, DNA adducts, DNA strand breaks, and alkali-labile sites. Streptozotocin damages chromosomes and produces DNA, both of which are facilitated by free radicals.[1]
Streptozotocin is toxic to pancreatic beta cell. The INS-1 murine pancreatic beta cell line undergoes apoptosis when exposed to 15 mM streptozotocin for one hour, followed by a 24-hour recovery period. The cells experience both apoptosis (17%) and necrosis (22%) when exposed to 30 mM streptozotocin.[2]

ln Vivo

Streptozotocin is frequently used to induce diabetes mellitus in experimental animals. The low-affinity GLUT 2 glucose transporter allows streptozotocin to accumulate in pancreatic beta cells in a selective manner. A 4-month injection of streptozotocin (60 mg/kg) causes cataract development, a rapid degranulation of beta cells without necrosis, and an accumulation of glycogen in the kidney's proximal convoluted tubules. Rats with “Streptozotocin diabetes” exhibit persistent small, possibly secretory granules in the Golgi zone of beta cells, and lesions in the pancreatic exocrine cells at 100 mg/kg.[3]
Streptozotocin has been shown to cause cancer in rats, mice and hamster. In hamsters, a solitary dose of streptozotocin can cause tumors in the kidney, liver, pancreas, uterus, and liver. Normotensive Wistar Kyoto rats (WKY) given intraperitoneal injections of streptozotocin (100–150 mg/kg) for a year cause carcinogenesis, with tumor incidence in the liver being 70%, the kidneys 20%, and the liver and kidneys 10%.[4]

Enzyme Assay

Streptozocin is an effective agent that methylates DNA; in HL60, K562, and C1498 cells, it has IC50 values of 11.7, 904, and 1024 μg/mL, respectively.

Cell Assay

In 96-well plates, human and murine cell lines are cultivated in triplicate at a density of 2×10⁴ cells/well, either in the absence (untreated control) or in the presence of different concentrations of ALX (20-3000 μg/mL) or STZ (1-3000 μg/mL) for 48 hours at 37°C in a humidified atmosphere with 5% CO₂. The experiments used cells cultured in complete medium as a control, and cells cultured in dH₂O at a final concentration of 0.1% as a control for solvent toxicity. The MTT assay is used in accordance with the manufacturer's instructions to determine the effects of the tested drugs on the growth or viability of tumor cells. GraphPad Prism 4 is used to calculate the IC50 values, or drug concentrations that cause a 50% inhibition of cell growth.

Animal Protocol

Mice: The mice used are male C57BL/6 (10–16 weeks).The age distribution of the mice treated with Streptozocin and ALX, along with the controls, is as follows: n = 7 for Streptozocin xenograft, n = 11 for ALX xenograft, n = 7 for Streptozocin non-transplanted, n = 15 for ALX non-transplanted, and n = 7 for Streptozocin non-transplanted.Male C57BL/6 mice are given an inhalation anesthetic injection via the penile vein, consisting of either 180 mg/kg of streptozocin or 75 mg/mL of ALX. Male C57BL/6 mice make up the control group. Before the drug injection, six hours later, and every day after, blood glucose levels and body weight are recorded.
Rats: To induce menopause, thirty rats had their ovaries removed. Streptozocin (50 mg/kg) is injected intraperitoneally into rats one week following oophorectomy to cause diabetes mellitus (DM). Three days following the administration of streptozocin, blood glucose levels are measured; values greater than 250 mg/dL are regarded as positive for diabetes.

References

[1]. Mutat Res . 2002 Dec;512(2-3):121-34.

[2]. Biochem Mol Biol Int . 1996 Aug;39(6):1229-36.

[3]. Diabetes . 1967 Jan;16(1):51-6.

[4]. Tohoku J Exp Med . 1989 Oct;159(2):83-90.

[5]. Comp Med . 2011 Aug;61(4):356-60.

[6]. Anal Biochem . 2016 Jul 1:504:38-40.

These protocols are for reference only. InvivoChem does not independently validate these methods.

Physicochemical Properties

Molecular Formula

C8H15N3O7

Molecular Weight

265.22

Exact Mass

265.09

Elemental Analysis

C, 36.23; H, 5.70; N, 15.84; O, 42.23

CAS #

18883-66-4

Related CAS #

18883-66-4

Appearance

Solid powder

SMILES

CN(C(=O)N[C@@H]1[C@H]([C@@H]([C@H](O[C@@H]1O)CO)O)O)N=O

InChi Key

ZSJLQEPLLKMAKR-GKHCUFPYSA-N

InChi Code

InChI=1S/C8H15N3O7/c1-11(10-17)8(16)9-4-6(14)5(13)3(2-12)18-7(4)15/h3-7,12-15H,2H2,1H3,(H,9,16)/t3-,4-,5-,6-,7+/m1/s1

Chemical Name

1-methyl-1-nitroso-3-[(2S,3R,4R,5S,6R)-2,4,5-trihydroxy-6-(hydroxymethyl)oxan-3-yl]urea

Synonyms

NSC-85998; U9889; AI3-50821; NRRL 2697; U-9889; U 9889; NCI-C03167; NSC-85998; STZ; SZC; SZN; Zanosar

HS Tariff Code

2934.99.9001

Storage

Powder -20°C 3 years

4°C 2 years

In solvent -80°C 6 months

-20°C 1 month

Shipping Condition

Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Solubility Data

Solubility (In Vitro)

DMSO: 53~250 mg/mL (199.8~942.6 mM)

Water: ~53 mg/mL (~199.8 mM)

Ethanol: <1 mg/mL

Solubility (In Vivo)

Saline: 30 mg/mL

(Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.7705 mL	18.8523 mL	37.7045 mL
	5 mM	0.7541 mL	3.7705 mL	7.5409 mL
	10 mM	0.3770 mL	1.8852 mL	3.7705 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.

Calculator

Mass

Concentration

Volume

Molecular Weight*

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Molarity Calculator allows you to calculate the mass, volume, and/or concentration required for a solution, as detailed below:

Calculate the Mass of a compound required to prepare a solution of known volume and concentration
Calculate the Volume of solution required to dissolve a compound of known mass to a desired concentration
Calculate the Concentration of a solution resulting from a known mass of compound in a specific volume

See Example

An example of molarity calculation using the molarity calculator is shown below:
What is the mass of compound required to make a 10 mM stock solution in 5 ml of DMSO given that the molecular weight of the compound is 350.26 g/mol?

Enter 350.26 in the Molecular Weight (MW) box
Enter 10 in the Concentration box and choose the correct unit (mM)
Enter 5 in the Volume box and choose the correct unit (mL)
Click the “Calculate” button
The answer of 17.513 mg appears in the Mass box. In a similar way, you may calculate the volume and concentration.

Concentration (Start)

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Concentration (End)

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Dilution Calculator allows you to calculate how to dilute a stock solution of known concentrations. For example, you may Enter C1, C2 & V2 to calculate V1, as detailed below:

What volume of a given 10 mM stock solution is required to make 25 ml of a 25 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=25 μM, V2=25 ml and V1 is the unknown:

Enter 10 into the Concentration (Start) box and choose the correct unit (mM)
Enter 25 into the Concentration (End) box and select the correct unit (mM)
Enter 25 into the Volume (End) box and choose the correct unit (mL)
Click the “Calculate” button
The answer of 62.5 μL (0.1 ml) appears in the Volume (Start) box

Molecular formula

Total molecular weight

g/mol

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Molecular Weight Calculator allows you to calculate the molar mass and elemental composition of a compound, as detailed below:

Note: Chemical formula is case sensitive: C12H18N3O4 c12h18n3o4
Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter the chemical/molecular formula and click the “Calculate’ button.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (or molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Volume (to add to vial)

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Reconstitution Calculator allows you to calculate the volume of solvent required to reconstitute your vial.

Enter the mass of the reagent and the desired reconstitution concentration as well as the correct units
Click the “Calculate” button
The answer appears in the Volume (to add to vial) box

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal to make allowance for loss during the experiment)

Dosage mg/kg

Average weight of animals g

Dosing volume per animal μL

Number of animals

Step 2: Enter in vivo formulation (This is only a calculator, not the exact formulation for a specific product. Please contact us first if there is no in vivo formulation in the solubility section.)

% DMSO

% Tween 80

% ddH₂O

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Calculation results

Working concentration： mg/mL；

Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O，mix and clarify.

Note： (1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.

Clinical Trial Information

NCT Number	Recruitment	interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT03351296	Recruiting	Drug: Streptozocin Drug: Capecitabine	Pancreatic Cancer	Gustave Roussy, Cancer Campus, Grand Paris	June 26, 2018	Phase 2
NCT00448136	Completed	Drug: Streptozotocin Drug: 5 FU	Neoplasms	Hoffmann-La Roche	July 2007	Phase 2
NCT00004688	Completed	Drug: streptozocin Drug: mercaptopurine	Mixed Gliomas Anaplastic Astrocytoma	Emory University	August 1996	Phase 2
NCT00602082	Completed	Drug: cisplatin Drug: streptozocin	Gastrointestinal Carcinoid Tumor Islet Cell Tumor	Cambridge University Hospitals NHS Foundation Trust	August 2005	Phase 2

Biological Data

Blood glucose values (top row) and body weight (bottom row) in response to insulin (glargine) treatment (U/kg daily) after diabetes induction by using STZ in male nude mice from Charles River Laboratories (CRL), Jackson Laboratories (JAX), and Taconic Farms (TAC). Comp Med . 2011 Aug;61(4):356-60.