Size | Price | Stock | Qty |
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250mg |
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500mg |
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1g |
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Other Sizes |
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STING inhbitor 2 ( SN-011; SN011) is a novel and potent inhibitor of STING with potential anti-inflammatory activity. It can block the activation of the Sting signaling pathway.
ln Vitro |
Significant inhibition of STING stimulus-induced Ifnb, Cxcl10, and Il6 mRNA expression in transcription factor fibroblasts (MEFs) is observed upon treatment with SN-011 (1 μM; sheared for 6 hours) [1]. SN-011 (0.001-10 μM; pre-treatment for 6 hours) inhibits the expression of Ifnb induced by 2′3′-cGAMP in MEFs, mouse bone marrow-derived macrophages (BMDM), and human epithelial fibroblasts (HFF); IC50s are 127.5, 107.1, and 502.8 nM, respectively [SN-011 (1 μM; rest for 3 hours) inhibits the phosphorylation and oligomerization of 2'3'-cGAMP in HFF [1]. When STING ER-to-Golgi translocation is brought on by HSV-1 infection (4 hours), HT-DNA (1 hour), or 2' 3'-cGAMP stimulation (30 minutes), SN-011 (1 μM) inhibits it [1].
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ln Vivo |
In addition to preventing Trex1/mice from dying, SN-011 (5 mg/kg; intraperitoneally injected three times a week for one month) significantly suppresses markers of regulation and autoimmunity [1].
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Cell Assay |
Western Blot Analysis[1]
Cell Types: Human foreskin fibroblasts Tested Concentrations: 1 μM Incubation Duration: Pretreatment, then stimulated with 2'3'-cGAMP for 1 hour Experimental Results: Inhibition of 2'3'-cGAMP-induced STING oligomerization and phosphorylation. |
Animal Protocol |
Animal/Disease Models: 4-wk -old Trex1−/− mice [1]
Doses: 5 mg/kg Route of Administration: intraperitoneal (ip) injection 3 times per week for one month Experimental Results: The survival rate of mice increased. Reduce severe multi-organ inflammation. Serum antinuclear antibodies were diminished. |
References |
[1]. Hong Z, et, al. STING inhibitors target the cyclic dinucleotide binding pocket. Proc Natl Acad Sci U S A. 2021 Jun 15;118(24):e2105465118.
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Molecular Formula |
C25H19FN2O4S
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Molecular Weight |
462.4954
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Exact Mass |
462.105
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CAS # |
2249435-90-1
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SMILES |
S(C1C([H])=C([H])C(=C([H])C=1[H])F)(N([H])C1=C(C([H])=C([H])C(=C1[H])N([H])C(C1C([H])=C([H])C(=C([H])C=1[H])C1C([H])=C([H])C([H])=C([H])C=1[H])=O)O[H])(=O)=O
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Chemical Name |
N-(3-((4-fluorophenyl)sulfonamido)-4-hydroxyphenyl)-[1,1'-biphenyl]-4-carboxamide
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Synonyms |
SN 011 SN-011 SN011
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO : ~100 mg/mL (~216.22 mM)
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (5.41 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (5.41 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 2.1622 mL | 10.8108 mL | 21.6216 mL | |
5 mM | 0.4324 mL | 2.1622 mL | 4.3243 mL | |
10 mM | 0.2162 mL | 1.0811 mL | 2.1622 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.