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Description: Sodium salicylate, the sodium salt of salicylic acid, is a novel metabolite of acetylsalicylic acid, and functions by inhibiting NF-kB and reduces oxidative stress.
References: Science. 1994 Aug 12;265(5174):956-9; Proc Natl Acad Sci U S A. 1997 Apr 1;94(7):2869-73.
Chemical Name: Benzoic acid, 2-hydroxy-, monosodium salt
InChi Key: ABBQHOQBGMUPJH-UHFFFAOYSA-M
InChi Code: InChI=1S/C7H6O3.Na/c8-6-4-2-1-3-5(6)7(9)10;/h1-4,8H,(H,9,10);/q;+1/p-1
SMILES Code: O=C([O-])C1=CC=CC=C1O.[Na+]
Sodium salicylate; Magsalyl; Kerasalicyl; Kerosal;
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2
In vitro activity: Sodium salicylate acts as non-steroidal anti-inflammatory drug (NSAID), and induces apoptosis in cancer cells and also necrosis. Sodium salicylate inhibits the activation of NF-kappa B, and also inhibits NF-kappa B-dependent transcription from the Ig kappa enhancer and the human immunodeficiency virus (HIV) long terminal repeat (LTR) in transfected T cells. Sodium salicylate also induces apoptosis via p38 MAPK and inhibits TNF-induced JNK/stress-activated protein kinase activation.
Kinase Assay: Human purified COX-2 are and the cofactors Glutathione (5 mM), Adrenaline (5 mM), and Hematin (1 μM) are dissolved in 50 mM Tris buffer (pH 7.5). Hematin is first dissolved in a concentrated stock of 100 mM in 1 M NaOH before being further diluted in Tris buffer. Enzyme reactions are carried out in individual wells of 96-well plates with a final reaction volume of 200 μL. Different concentrations of Sodium Salicylate are added to the plate, followed by the addition of 10 units of enzyme (180 μL). The plates are incubated at 37° for 30 min before Arachidonic acid (10 nM to 30 μM) is added for a further 15 min. The reaction is stopped by heating the plate to 100°C for 5 min. The 96-well plate is then centrifuged at 10,000 × g for 10 min, and appropriated samples are removed and added into the radioimmunoassay
Cell Assay: To assess the direct effect of Sodium Salicylate on COX-2 activity after induction has occurred, A549 cells are first treated with IL-1β for 24 hr, and the culture medium is replaced with DMEM containing different concentrations of Sodium Salicylate(10, 100 and 1000 μg/mL). Cells are incubated at 37°C for 30 min. Arachidonic acid (1-30 μM) is then added for 15 min, and the medium is removed for the measurement of PGE2
Science. 1994 Aug 12;265(5174):956-9; Proc Natl Acad Sci U S A. 1997 Apr 1;94(7):2869-73.
Purity ≥98%
COA
MSDS