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Sodium orthovanadate, an inorganic compound with chemical formula of Na3VO4, is a potent inhibitor for several enzymes such as protein tyrosine phosphatases (PTPs), alkaline phosphatase (ALP), and ATPase. It displayed a variety of biological activities, partly due to its structural mimics of phosphates. The inhibitory effects against the aforementioned enzymes can be reversed by dilution or the addition of Ethylenediaminetetraacetic acid (EDTA).
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Sodium orthovanadate (vanadate) also starts to polymerize at concentrations greater than 0.1 mM at neutral pH. The yellow-orange solutions of decavanadate can be converted to the colorless solutions of monomeric Sodium orthovanadate (vanadate) by dilution after a period of many hours. In the presence of oxidizing agents, vanadium ions exist as the hydrated monomer of Sodium orthovanadate (vanadate: HVO42- or H2VO4-) at micromolar concentrations near neutral pH. Boiling at pH 10 speeds up the process by promoting the kinetically slow depolymerization process[1]. The phosphorylation state of ASK1 at serine 83 and threonine 845 caused by ischemia may be changed by sodium orthovanadate. During cerebral ischemia, sodium orthovanadate may raise the tyrosine posphorylation of PTEN and further limit the activation of ASK1 by activating Akt[2].
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| References |
[1]. Gordon JA, et al. Use of vanadate as protein-phosphotyrosine phosphatase inhibitor. Methods Enzymol. 1991;201:477-82.
[2]. Wu DN, et al. Down-regulation of PTEN by sodium orthovanadate inhibits ASK1 activation via PI3-K/Akt during cerebral ischemia in rat hippocampus. Neurosci Lett. 2006 Aug 14;404(1-2):98-102. |
| Molecular Formula |
NA3O4V
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| Molecular Weight |
183.91
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| CAS # |
13721-39-6
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| SMILES |
[V](=O)([O-])([O-])[O-].[Na+].[Na+].[Na+]
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
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| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~8.33 mg/mL (~45.29 mM)
DMSO :< 1 mg/mL |
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| Solubility (In Vivo) |
Solubility in Formulation 1: 16.67 mg/mL (90.64 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication (<60°C).
Solubility in Formulation 2: Saline:30mg/mL (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.4374 mL | 27.1872 mL | 54.3744 mL | |
| 5 mM | 1.0875 mL | 5.4374 mL | 10.8749 mL | |
| 10 mM | 0.5437 mL | 2.7187 mL | 5.4374 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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