| Size | Price | Stock | Qty |
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| 500mg |
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| Other Sizes |
| Targets |
Sodium D-Pantothenate is a precursor in the biosynthesis of coenzyme A (CoA), a vital cofactor in numerous metabolic pathways. CoA is essential for the synthesis and metabolism of proteins, carbohydrates, and fats. By providing the building blocks for CoA synthesis, the compound supports a wide range of enzymatic reactions central to energy metabolism and cellular function.
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| ln Vitro |
As the precursor of coenzyme A, D-sodium pantothenate mostly contributes to the pyramid cycle and energy production via the β-oxidation pathway and TCA cycle, respectively [1].
In vitro, Sodium D-Pantothenate is used to study cell metabolism and CoA activity. It is a common supplement in cell culture media to support cell growth and proliferation. Its effects are not typically measured as a direct activity but rather as a nutritional component that enables metabolic processes. |
| ln Vivo |
Valproic acid (VPA; 300, 400, and 500 mg/kg, sc)-induced conductive neural tube abnormalities are lessened by pantothenic acid (PTA; 3x10, 3x100, and 3x300 mg/kg) [2].
In vivo, Sodium D-Pantothenate is an essential nutrient for several types of animals. It is used as a vitamin supplement to prevent or treat pantothenic acid deficiency. It is also an ingredient in some skin or hair products. Its primary role is to support CoA synthesis and energy metabolism. |
| Enzyme Assay |
In vitro enzyme/receptor binding assays are not applicable for Sodium D-Pantothenate, as it is a vitamin and a metabolic precursor rather than a compound that targets specific enzymes or receptors. Its activity is assessed indirectly by measuring CoA levels or the activity of CoA-dependent enzymes in cells cultured with or without the vitamin.
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| Cell Assay |
In vitro cellular assays for Sodium D-Pantothenate typically involve culturing cells in media with varying concentrations of the compound to assess its effects on cell growth, proliferation, and metabolism. The compound's role as a nutrient is evaluated by measuring cell viability, CoA levels, or the activity of CoA-dependent enzymes.
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| Animal Protocol |
Animal/Disease Models: Female ICR mice, body weight 29 -35 g[2]
Doses: 3x10, 3x100 and 3x300 mg/kg (10 mL/kg, dosing volume) Route of Administration: intraperitonealon day 8.5 of gestation Injection Experimental Results: VPA Dramatically diminished (300, 400, and 500 mg/kg, sc) the risk of extracerebral malformations, while other external malformations (e.g., open eyelids) or skeletal malformations (e.g., fused, missing, or bifurcated ribs and thoracic spine fusion and sternal fusion) were not diminished. In vivo animal models for Sodium D-Pantothenate are used to study the effects of pantothenic acid deficiency and supplementation. Animals are fed diets with controlled levels of the vitamin, and physiological parameters such as growth, reproduction, and metabolic health are assessed. |
| ADME/Pharmacokinetics |
Absorption, Distribution and Excretion
Pantothenic acid is distributed very rapidly from plasma to the liver; approximately 80% of the dose is cleared from plasma within 10 minutes after intravenous injection. In rats, oral administration of 21.6 μM/kg pantothenic acid resulted in higher total plasma pantothenic acid levels than oral administration of calcium pantothenate, but there was no difference in the amount of conjugated pantothenic acid. Both groups reached peak plasma concentrations 2–4.5 hours after ingestion. Twenty-four hours after oral administration of pantothenic acid and calcium pantothenate to rats, the total pantothenic acid excretion was 29% and 18%, respectively. Pantothenic acid is more readily absorbed from the gastrointestinal tract than calcium pantothenate. Sodium D-Pantothenate has a molecular formula of C9H16NO5Na and a molecular weight of 241.22 g/mol. It is a white powder to crystal with a specific rotation of +25.0 to +28.5° (c=5, H2O). The compound is highly soluble in water and should be stored under appropriate conditions to maintain stability. |
| Toxicity/Toxicokinetics |
Sodium D-Pantothenate is generally recognized as safe (GRAS) and is well-tolerated when used as a vitamin supplement. As a water-soluble vitamin, excess amounts are typically excreted in the urine, and toxicity is low. It is not intended for therapeutic use as a drug but is used as a nutritional supplement.
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| References |
[1]. Shuai Chen, et al. Metabolomic analysis of the toxic effect of chronic exposure of cadmium on rat urine. Environ Sci Pollut Res Int. 2018 Feb;25(4):3765-3774.
[2]. M Sato, et al. Pantothenic acid decreases valproic acid-induced neural tube defects in mice (I). Teratology. 1995 Sep;52(3):143-8. |
| Additional Infomation |
Mechanism of Action
To investigate the functional effects of pantothenic acid on dermal fibroblasts, we cultured cells and performed in vitro proliferation assays using a standardized scratch assay procedure. In all three donors, a concentration of 20 μg/mL of pantothenic acid significantly stimulated the proliferation of cultured dermal fibroblasts. To investigate the molecular mechanism by which pantothenic acid promotes cell proliferation, we analyzed gene expression in dermal fibroblasts cultured with 20 μg/mL pantothenic acid compared to untreated cells using a GeneChip Human Exon 1.0 ST Array. We identified several significantly regulated genes, including those encoding interleukin (IL)-6, IL-8, Id1, HMOX-1, HspB7, CYP1B1, and MARCH-II. We subsequently validated the regulation of these genes using quantitative real-time polymerase chain reaction (qRT-PCR). Immunoblot analysis confirmed at the protein level that pantothenic acid and pantothenic acid induce HMOX-1 expression in dermal cells. Functional studies have shown that panthenol enhances the inhibition of free radical generation in cultured skin fibroblasts. In summary, these studies provide new insights into the molecular mechanisms by which pantothenic acid and panthenol stimulate dermal fibroblast proliferation. /Calcium Pantothenate/ Therapeutic Uses Topical application of calcium pantothenate is widely used clinically to promote wound healing. Sodium D-Pantothenate (CAS# 867-81-2) is the sodium salt of vitamin B5 and a precursor in the biosynthesis of coenzyme A (CoA). It is an essential nutrient used in research to study cellular metabolism. The compound is for research use only and is not intended for human therapeutic use as a drug, though it is used as a nutritional supplement. |
| Molecular Formula |
C9H16NNAO5
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|---|---|
| Molecular Weight |
241.21
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| Exact Mass |
241.092
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| CAS # |
867-81-2
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| Related CAS # |
D-Pantothenic acid;79-83-4;Pantothenic acid-13C3,15N hemicalcium;356786-94-2;D-Pantothenic acid hemicalcium salt;137-08-6
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| PubChem CID |
23679004
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| Appearance |
Typically exists as solid at room temperature
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| Boiling Point |
551.5ºC at 760 mmHg
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| Melting Point |
171-178 °C(lit.)
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| Flash Point |
287.3ºC
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| Index of Refraction |
27 ° (C=5, H2O)
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
6
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| Heavy Atom Count |
16
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| Complexity |
244
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| Defined Atom Stereocenter Count |
1
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| SMILES |
[C@H](O)(C(=O)NCCC(=O)O)C(C)(C)CO.[Na]
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| InChi Key |
GQTHJBOWLPZUOI-FJXQXJEOSA-M
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| InChi Code |
InChI=1S/C9H17NO5.Na/c1-9(2,5-11)7(14)8(15)10-4-3-6(12)13;/h7,11,14H,3-5H2,1-2H3,(H,10,15)(H,12,13);/q;+1/p-1/t7-;/m0./s1
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| Chemical Name |
sodium;3-[[(2R)-2,4-dihydroxy-3,3-dimethylbutanoyl]amino]propanoate
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| Synonyms |
Sodium D Pantothenate; Sodium D-Pantothenate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~100 mg/mL (~414.56 mM)
DMSO : ~5 mg/mL (~20.73 mM) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 0.5 mg/mL (2.07 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 5.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 0.5 mg/mL (2.07 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 5.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: ≥ 0.5 mg/mL (2.07 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. Solubility in Formulation 4: 120 mg/mL (497.47 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.1458 mL | 20.7288 mL | 41.4577 mL | |
| 5 mM | 0.8292 mL | 4.1458 mL | 8.2915 mL | |
| 10 mM | 0.4146 mL | 2.0729 mL | 4.1458 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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