| Size | Price | Stock | Qty |
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| 10g |
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| 25g |
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| 50g |
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| Other Sizes |
Purity: ≥98%
| Targets |
Calcium D-Pantothenate is a vitamin that serves as a precursor to coenzyme A (CoA) and acyl carrier protein. As a component of CoA, it is essential for the metabolism of carbohydrates, fats, and proteins. CoA is involved in the citric acid cycle and fatty acid synthesis. As a component of acyl carrier protein, it is involved in the synthesis of fatty acids. By providing pantothenic acid, the compound supports numerous metabolic pathways essential for energy production and the synthesis of important biomolecules.
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| ln Vitro |
Pantothenic acid increases mainly the content of free glutathione, with little effect on protein-boundglutathione in human lymphoblastoic (Jurkat) cells. Pantothenic acid also increases cell respiration with pyruvate as the exogenous substrate. Pantothenic acid belongs to vitamin B group and is the building stone of coenzyme A. Pantothenic acid can be therefore infer that its beneficial effect in various kinds of cell damage by ROS is related to the increased content or stimulated biosynthesis of this coenzyme. Pantothenic acid increases the glutathione content by more than 50% and increases the GSH/GSSG ratio by approximately the same factor. Pantothenic acid and N-acetylcysteine alleviates the ultraviolet-induced decrease of glutathione content, diminished lipid peroxidation, and partly protects the cells against apoptosisproduced by ultraviolet irradiation.
Cell Assay: Pantothenic acid (1 mM) increased biosynthesis of glutathione by boosting cell energetic in Jurkat cells. In addition, pantothenic acid (1 mM) protected against oxidative damage and apoptosis in Ehrlich ascites tumour cells. In vitro, Calcium D-Pantothenate is used as a source of pantothenic acid in cell culture media. Pantothenic acid is essential for the growth and proliferation of many cell types. It is a component of CoA, which is required for numerous metabolic reactions. Cell-based assays can be used to study the effects of pantothenic acid deficiency and supplementation on cellular metabolism and function. |
| ln Vivo |
Pantothenic acid-treated rats exhibited higher basal levels of corticosterone and progesterone than control rats in Adrenal cells. The response of ACTH and/or PRL on corticosterone and progesterone release is higher in the pantothenic acid-treated rats than in the control rats. Pantothenic acid supplementation stimulates the ability of adrenal cells in male rats to secrete corticosterone and progesterone. Pantothenic acid supplementation induces adrenalhyperresponsiveness to ACTH stimulation, and PRL further stimulated adrenal sensitivity to ACTH. Pantothenic acid prior to valproic acid (VPA) significantly protects against VPA-induced neural tube defects (NTDs) in CD-1mice. Pantothenic acid prevents VPA-induced alterations in NF-kappaB, Pim-1, and c-Myb in CD-1 mice. |
| Enzyme Assay |
Non-cellular assays for Calcium D-Pantothenate typically involve its use as a nutrient in microbiological assays. For example, it can be used to support the growth of specific microorganisms, and the degree of growth can be used to quantify the pantothenic acid content in a sample. These assays are based on the essential role of pantothenic acid in microbial metabolism.
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| Cell Assay |
In vitro cell-based assays for Calcium D-Pantothenate are used to study its role in cellular metabolism. Cell culture media often contain pantothenic acid as an essential nutrient. Experiments can be designed to deplete cells of pantothenic acid and then study the effects of its re-addition on cell growth, metabolism, and gene expression. These studies help to elucidate the role of CoA and pantothenic acid in cellular function.
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| Animal Protocol |
3.39-10.54 mg/kg, diets | Pantothenic acid (200 mg/kg, intraperitoneal injections) completely blocked valproic acid-induced neural tube defects and the decreases in c-Myb and Pim-1 protein expression in CD-1 mice. Moreover, Calcium D-pantothenate (3.39-10.54 mg/kg, diets) increased the intestinal enzymes activities and docosahexaenoic acid (DHA) levels.
| ADME/Pharmacokinetics |
Calcium D-Pantothenate is an orally available nutrient. It has a molecular weight of 476.53 g/mol. It is well-absorbed from the gastrointestinal tract and is distributed throughout the body. It is converted to CoA in cells. Excess pantothenic acid is excreted in the urine. Its pharmacokinetics are well-characterized as a normal nutrient.
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| Toxicity/Toxicokinetics |
Calcium D-Pantothenate is generally recognized as safe (GRAS) and is well-tolerated at dietary supplement doses. It has a very low toxicity profile. No significant adverse effects are associated with its use at recommended levels. It is an approved food additive and dietary supplement ingredient.
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| References |
FEBS Lett.2004 Jul 2;569(1-3):169-72;Biofactors.2003;17(1-4):61-73.
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| Additional Infomation |
Calcium pantothenate is a polymer. It is a water-soluble calcium salt of vitamin B5, widely found in animal and plant tissues, and possesses antioxidant properties. Pantothenate is a component of coenzyme A (CoA) and also part of the vitamin B2 complex. Vitamin B5 is a growth factor essential for various metabolic functions, including the metabolism of carbohydrates, proteins, and fatty acids. This vitamin also participates in the synthesis of cholesterol, lipids, neurotransmitters, steroid hormones, and hemoglobin. Butyryl-β-alanine can also be considered a complex of pantothenate and β-alanine. It is integrated into coenzyme A and protects cells from oxidative damage by increasing glutathione levels.
Calcium D-Pantothenate is the calcium salt of vitamin B5, an essential nutrient. It is a precursor to coenzyme A, which is critical for energy metabolism and the synthesis of fatty acids and other biomolecules. It is used as a dietary supplement to prevent or treat deficiency and is generally recognized as safe. Calcium D-Pantothenate is not a drug but a nutrient. |
| Molecular Formula |
C18H32CAN2O10
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| Molecular Weight |
476.53
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| Exact Mass |
476.168
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| CAS # |
137-08-6
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| Related CAS # |
D-Pantothenic acid;79-83-4;D-Pantothenic acid sodium;867-81-2
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| PubChem CID |
443753
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| Appearance |
White to off-white solid powder
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| Density |
1.266g/cm3
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| Boiling Point |
551.5ºC at 760 mmHg
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| Melting Point |
190 °C
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| Flash Point |
145 °C
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| Index of Refraction |
27 ° (C=5, H2O)
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| Hydrogen Bond Donor Count |
6
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| Hydrogen Bond Acceptor Count |
10
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| Rotatable Bond Count |
10
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| Heavy Atom Count |
31
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| Complexity |
233
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| Defined Atom Stereocenter Count |
2
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| SMILES |
CC(C)(CO)[C@H](C(=O)NCCC(=O)[O-])O.CC(C)(CO)[C@H](C(=O)NCCC(=O)[O-])O.[Ca+2]
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| InChi Key |
FAPWYRCQGJNNSJ-UBKPKTQASA-L
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| InChi Code |
InChI=1S/2C9H17NO5.Ca/c2*1-9(2,5-11)7(14)8(15)10-4-3-6(12)13;/h2*7,11,14H,3-5H2,1-2H3,(H,10,15)(H,12,13);/q;;+2/p-2/t2*7-;/m00./s1
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| Chemical Name |
calcium;3-[[(2R)-2,4-dihydroxy-3,3-dimethylbutanoyl]amino]propanoate
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| Synonyms |
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
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| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (10.49 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (10.49 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: 100 mg/mL (419.69 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.0985 mL | 10.4925 mL | 20.9850 mL | |
| 5 mM | 0.4197 mL | 2.0985 mL | 4.1970 mL | |
| 10 mM | 0.2099 mL | 1.0493 mL | 2.0985 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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