| Size | Price | Stock | Qty |
|---|---|---|---|
| 25g |
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| Other Sizes |
| Targets |
As a histological stain and pH indicator, Sodium alizarinesulfonate does not have a specific biological target in the context of a pharmacological drug. Its primary mechanism of action is based on its chemical properties as a dye that binds to calcium deposits in tissues. It is used to determine the presence of calcific deposition by cells of an osteogenic lineage.
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|---|---|
| ln Vitro |
In vitro, Sodium alizarinesulfonate is used as a chromogenic agent for the colorimetric determination of various compounds, such as dothiepin hydrochloride in pharmaceutical formulations. It is also employed in biochemical assays to quantitatively determine the presence of calcific deposition by cells of an osteogenic lineage.
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| ln Vivo |
In vivo, Sodium alizarinesulfonate is used as a histological dye to stain the skeletal remains of embryos in cleared whole-mount specimens. It is typically used in small mammals for this purpose. It is not used as a therapeutic agent in vivo.
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| Enzyme Assay |
In vitro enzyme/receptor binding assays are not applicable for Sodium alizarinesulfonate, as it is a histological stain and chemical reagent, not a compound that targets specific enzymes or receptors. Its utility is based on its chemical properties as a dye that binds to calcium and its use as a pH indicator.
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| Cell Assay |
In vitro cellular assays for Sodium alizarinesulfonate involve staining cells or tissues to detect calcium deposits. The Alizarin Red S staining method is widely used to evaluate osteogenic differentiation in mesenchymal stem cells and other cell types. Cells are fixed and incubated with the dye, and the formation of red-stained mineralized nodules is quantified.
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| Animal Protocol |
In vivo animal models for Sodium alizarinesulfonate involve its use as a staining agent to visualize skeletal development in embryos. The dye is administered to pregnant animals, and it is taken up by calcifying tissues in the developing fetus, allowing for the visualization of the skeleton in cleared whole-mount specimens.
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| ADME/Pharmacokinetics |
Sodium alizarinesulfonate has a molecular formula of C14H7NaO7S and a molecular weight of 342.26 g/mol. It is an orange-yellow powder that is freely soluble in water. The compound is also known as Alizarin Red S, Mordant Red 3, and Alizarin Carmine. It is a histological dye and a pH indicator.
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| Toxicity/Toxicokinetics |
Sodium alizarinesulfonate is a histological stain and pH indicator with a well-established safety profile for laboratory use. As a dye, it is not intended for human consumption or therapeutic use. Standard laboratory safety practices should be followed when handling this compound, including the use of personal protective equipment.
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| References | |
| Additional Infomation |
Alizarin Red S is an organosodium salt with the counterion 3,4-dihydroxy-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate. It is commonly used to stain the skeletal remains of embryos in cleared, fully mount specimens, and is typically used in small mammals. It is a histological dye. It is both an organosodium salt and an organosulfonate. It contains the 3,4-dihydroxy-9,10-dioxo-9,10-dihydroanthracene-2-sulfonate group.
Sodium alizarinesulfonate (CAS# 130-22-3), also known as Alizarin Red S, is a histological dye and pH indicator. It is used to stain calcium deposits in cells and tissues, particularly for detecting osteogenic differentiation. It is also used as a chromogenic agent for colorimetric assays. The compound is for research use only and is not for human therapeutic use. |
| Molecular Formula |
C28H12CL2N2O4
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|---|---|
| Molecular Weight |
511.31
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| Exact Mass |
341.981
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| CAS # |
130-22-3
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| Related CAS # |
83-61-4 (Parent)
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| PubChem CID |
3955344
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| Appearance |
Light yellow to brown solid powder
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| LogP |
1.858
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
1
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| Heavy Atom Count |
23
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| Complexity |
599
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
HFVAFDPGUJEFBQ-UHFFFAOYSA-M
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| InChi Code |
InChI=1S/C14H8O7S.Na/c15-11-6-3-1-2-4-7(6)12(16)10-8(11)5-9(22(19,20)21)13(17)14(10)18;/h1-5,17-18H,(H,19,20,21);/q;+1/p-1
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| Chemical Name |
sodium;3,4-dihydroxy-9,10-dioxoanthracene-2-sulfonate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~12.5 mg/mL (~36.52 mM)
H2O : ~2 mg/mL (~5.84 mM) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.25 mg/mL (3.65 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.9558 mL | 9.7788 mL | 19.5576 mL | |
| 5 mM | 0.3912 mL | 1.9558 mL | 3.9115 mL | |
| 10 mM | 0.1956 mL | 0.9779 mL | 1.9558 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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