Skepinone-L (CBS3830)

Alias: CBS3830; CBS-3830; CBS 3830; Skepinone-L
Cat No.:V0486 Purity: ≥98%
Skepinone-L (CBS-3830; CBS3830)is a novel, potent, selective and ATP-competitive inhibitor p38α-MAPK with potential anti-inflammatory activity.
Skepinone-L (CBS3830) Chemical Structure CAS No.: 1221485-83-1
Product category: p38 MAPK
This product is for research use only, not for human use. We do not sell to patients.
Size Price Stock Qty
1mg
5mg
10mg
25mg
50mg
100mg
Other Sizes
Official Supplier of:
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text
Alternate Text

 

  • Business Relationship with 5000+ Clients Globally
  • Major Universities, Research Institutions, Biotech & Pharma
  • Citations by Top Journals: Nature, Cell, Science, etc.
Top Publications Citing lnvivochem Products
Purity & Quality Control Documentation

Purity: ≥98%

Product Description

Skepinone-L (CBS-3830; CBS3830) is a novel, potent, selective, and ATP-competitive p38-MAPK inhibitor that may have anti-inflammatory effects. With an IC50 of 5 nM, it inhibits p38α-MAPK.

Biological Activity I Assay Protocols (From Reference)
Targets
p38α (IC50 = 5 nM)
ln Vitro
Skepinone-L reduces TNF-α, IL-1β, and IL-10 concentrations, which are controlled by p38 MAPK, with an IC50 range of 30 to 50 nM and exhibits concentration-dependent inhibition of HSP27 (Ser82) phosphorylation through the p38 MAPK pathway.[1] Skepinone-L (1 μM) reduces platelet secretion and aggregation by inhibiting the phosphorylation of the platelet p38 MAPK substrate Hsp27 that is activated by stimulation with CRP, thrombin, or the thromboxane A2 analogue U-46619. [2]
ln Vivo
Skepinone-L inhibits TNF-induced release from Gal/LPS by 77% in living organisms. [1]
Enzyme Assay
Skepinone-L (CBS3830), the first ATP-competitive p38 MAPK inhibitor with excellent in vivo efficacy and selectivity. Therefore, Skepinone-L (CBS3830) is a valuable probe for chem. biol. research, and it may foster the development of a unique class of kinase inhibitors.
Animal Protocol
Mice
~3 mg/kg
Oral administration
References

[1]. Nat Chem Biol . 2011 Dec 25;8(2):141-3.

[2]. Cell Physiol Biochem . 2013;31(6):914-24.

These protocols are for reference only. InvivoChem does not independently validate these methods.
Physicochemical Properties
Molecular Formula
C24H21F2NO4
Molecular Weight
425.42
Exact Mass
425.14
Elemental Analysis
C, 67.76; H, 4.98; F, 8.93; N, 3.29; O, 15.04
CAS #
1221485-83-1
Appearance
Solid powder
SMILES
C1CC2=C(C=CC(=C2)NC3=C(C=C(C=C3)F)F)C(=O)C4=C1C=CC(=C4)OC[C@@H](CO)O
InChi Key
HXMGCTFLLWPVFM-GOSISDBHSA-N
InChi Code
InChI=1S/C24H21F2NO4/c25-16-4-8-23(22(26)10-16)27-17-5-7-20-15(9-17)2-1-14-3-6-19(11-21(14)24(20)30)31-13-18(29)12-28/h3-11,18,27-29H,1-2,12-13H2/t18-/m1/s1
Chemical Name
13-(2,4-difluoroanilino)-5-[(2R)-2,3-dihydroxypropoxy]tricyclo[9.4.0.03,8]pentadeca-1(11),3(8),4,6,12,14-hexaen-2-one
Synonyms
CBS3830; CBS-3830; CBS 3830; Skepinone-L
HS Tariff Code
2934.99.9001
Storage

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

Shipping Condition
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
Solubility Data
Solubility (In Vitro)
DMSO: ~85 mg/mL (~199.8 mM)
Water: <1 mg/mL
Ethanol: ~85 mg/mL (~199.8 mM)
Solubility (In Vivo)
0.5% methylcellulose: 30mg/mL
 (Please use freshly prepared in vivo formulations for optimal results.)
Preparing Stock Solutions 1 mg 5 mg 10 mg
1 mM 2.3506 mL 11.7531 mL 23.5062 mL
5 mM 0.4701 mL 2.3506 mL 4.7012 mL
10 mM 0.2351 mL 1.1753 mL 2.3506 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.

Calculator

Molarity Calculator allows you to calculate the mass, volume, and/or concentration required for a solution, as detailed below:

  • Calculate the Mass of a compound required to prepare a solution of known volume and concentration
  • Calculate the Volume of solution required to dissolve a compound of known mass to a desired concentration
  • Calculate the Concentration of a solution resulting from a known mass of compound in a specific volume
An example of molarity calculation using the molarity calculator is shown below:
What is the mass of compound required to make a 10 mM stock solution in 5 ml of DMSO given that the molecular weight of the compound is 350.26 g/mol?
  • Enter 350.26 in the Molecular Weight (MW) box
  • Enter 10 in the Concentration box and choose the correct unit (mM)
  • Enter 5 in the Volume box and choose the correct unit (mL)
  • Click the “Calculate” button
  • The answer of 17.513 mg appears in the Mass box. In a similar way, you may calculate the volume and concentration.

Dilution Calculator allows you to calculate how to dilute a stock solution of known concentrations. For example, you may Enter C1, C2 & V2 to calculate V1, as detailed below:

What volume of a given 10 mM stock solution is required to make 25 ml of a 25 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=25 μM, V2=25 ml and V1 is the unknown:
  • Enter 10 into the Concentration (Start) box and choose the correct unit (mM)
  • Enter 25 into the Concentration (End) box and select the correct unit (mM)
  • Enter 25 into the Volume (End) box and choose the correct unit (mL)
  • Click the “Calculate” button
  • The answer of 62.5 μL (0.1 ml) appears in the Volume (Start) box
g/mol

Molecular Weight Calculator allows you to calculate the molar mass and elemental composition of a compound, as detailed below:

Note: Chemical formula is case sensitive: C12H18N3O4  c12h18n3o4
Instructions to calculate molar mass (molecular weight) of a chemical compound:
  • To calculate molar mass of a chemical compound, please enter the chemical/molecular formula and click the “Calculate’ button.
Definitions of molecular mass, molecular weight, molar mass and molar weight:
  • Molecular mass (or molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
  • Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.
/

Reconstitution Calculator allows you to calculate the volume of solvent required to reconstitute your vial.

  • Enter the mass of the reagent and the desired reconstitution concentration as well as the correct units
  • Click the “Calculate” button
  • The answer appears in the Volume (to add to vial) box
In vivo Formulation Calculator (Clear solution)
Step 1: Enter information below (Recommended: An additional animal to make allowance for loss during the experiment)
Step 2: Enter in vivo formulation (This is only a calculator, not the exact formulation for a specific product. Please contact us first if there is no in vivo formulation in the solubility section.)
+
+
+

Calculation results

Working concentration mg/mL;

Method for preparing DMSO stock solution mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation:Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
             (2) Be sure to add the solvent(s) in order.

Biological Data
  • Skepinone-L abrogates activation-dependent Hsp27 phosphorylation in platelets. Cell Physiol Biochem . 2013;31(6):914-24.
  • Skepinone-L significantly affects activation-dependent platelet secretion and aggregation. Cell Physiol Biochem . 2013;31(6):914-24.
  • Agonist-induced Ca2+ influx in platelets in absence and presence of Skepinone-L. Cell Physiol Biochem . 2013;31(6):914-24.
  • Skepinone-L impairs phosphorylation/activation of platelet cytosolic phospholipase A2 (cPLA2) and platelet thromboxane synthesis. Cell Physiol Biochem . 2013;31(6):914-24.
  • Activation-dependent in vitro thrombus formation in absence and presence of Skepinone-L. Cell Physiol Biochem . 2013;31(6):914-24.
Contact Us