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    SH-4-54
    SH-4-54

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    This product is for research use only, not for human use. We do not sell to patients.
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    InvivoChem Cat #: V1385
    CAS #: 1456632-40-8Purity ≥98%

    Description: SH-4-54 (SH 454; SH4-54; SH454; SH-454) is a novel and potent STAT inhibitor with potential antineoplastic activity. It inhibits STAT3/5 with KD values of 300 nM and 464 nM, respectively. SH-4-54 shows potent anti-proliferative activity in vitro against glioblastoma brain cancer stem cells (BTSCs) and effectively suppresses STAT3 phosphorylation and its downstream transcriptional targets at low nanomolar concentrations.

    References: ACS Med Chem Lett. 2013 Sep 8;4(11):1102-7.

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    Molecular Weight (MW)610.59
    FormulaC29H27F5N2O5S
    CAS No.1456632-40-8
    Storage-20℃ for 3 years in powder form
    -80℃ for 2 years in solvent
    Solubility (In vitro)DMSO: 100 mg/mL (163.8 mM)
    Water: <1 mg/mL
    Ethanol: 50 mg/mL (81.9 mM)
    Other info

    Chemical Name: 4-(N-(4-cyclohexylbenzyl)-2-((2,3,4,5,6-pentafluoro-N-methylphenyl)sulfonamido)acetamido)benzoic acid

    InChi Key: VFPYGNNOSJWBHF-UHFFFAOYSA-N

    InChi Code: InChI=1S/C29H27F5N2O5S/c1-35(42(40,41)28-26(33)24(31)23(30)25(32)27(28)34)16-22(37)36(21-13-11-20(12-14-21)29(38)39)15-17-7-9-19(10-8-17)18-5-3-2-4-6-18/h7-14,18H,2-6,15-16H2,1H3,(H,38,39)

    SMILES Code: O=C(O)C1=CC=C(N(CC2=CC=C(C3CCCCC3)C=C2)C(CN(C)S(=O)(C4=C(F)C(F)=C(F)C(F)=C4F)=O)=O)C=C1           

    Synonyms

    SH 454; SH-4-54; SH4-54; SH-454; SH 4-54; SH454.


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    In Vitro

    In vitro activity: SH-4-54 shows unprecedented cytotoxicity in human glioblastoma brain cancer stem cells (BTSCs), while has no toxicity in human fetal astrocytes. In addition, SH-4-54 effectively suppresses STAT3 phosphorylation and its downstream transcriptional targets.


    Kinase Assay: The binding experiments are carried out on a ProteOn XPR36 biosensor at 25°C using the HTE sensor chip. The flow cells of the sensor chip are loaded with a nickel solution at 30 μL/min for 120 s to saturate the Tris–NTA surface with Ni(II) ions. Purified His-tagged STAT3 and STAT5 in PBST buffer (PBS with 0.005% (v/v) Tween-20 and 0.001% DMSO pH 7.4) is injected in the first and second channels of the chip respectively in the vertical direction at a flow rate of 25 μg/μL for 300 s, which attained, on average, ~8000 resonance unit (RU). After a wash with PBST buffer, inhibitors binding to the immobilized proteins is monitored by injecting a range of concentrations along with a blank at a flow rate of 100 μL/min for 200 s for each of these small molecules. When the injection of the small molecule inhibitor is completed, running buffer is allowed to flow over the immobilized substrates for the non-specifically bound inhibitors to dissociate for 600 s. Following dissociation of the inhibitors, the chip surface is regenerated with an injection of 1 M NaCl at a flow rate of 100 μL/ml for 18 s. Interspot channel reference is used for non-specific binding corrections and the blank channel used with each analyte injection served as a double reference to correct for possible baseline drift. Data are analyzed using ProteOn Manager Software version 3.1. The Langmuir 1:1 binding model was used to determine the KD values. 


    Cell Assay: BTSC spheres are dissociated to single cells with the enzyme Accumax, seeded at 1500 cells/ 96-well and treated with drug or vehicle (DMSO) one day after plating. Cytotoxicity studies are repeated independently using BTSC lines 25M, 67EF, 73EF, 84EF and 127EF. BTSC spheres are dissociated to single cells as above and plated in 96 well plates in triplicate at 3000 cells/ 96-well. In both sets of experiments drugs are used as serial dilutions within the range of 5 μM to 100 nM in the first set and 25 μM to 10 nM. Cell viability following drug treatment is assessed three days later using the alamarBlue assay according to the manufacturer’s instructions. All culture experiments are performed in triplicate with a minimum of three wells per condition.

    In VivoIn mice orthotopically xenografted with BT73, SH-4-54 (10 mg/kg i.p.) exhibits BBB permeability, potently suppresses glioma tumor growth, and inhibits pSTAT3.
    Animal modelNOD-SCID bearing ed with BT73 glioma xenografts 
    Formulation & DosageSuspended in 50% polyethylene glycol 300 in water; 10 mg/kg; i.p. injection 
    References

    ACS Med Chem Lett. 2013 Sep 8;4(11):1102-7.


    These protocols are for reference only. InvivoChem does not independently validate these methods.

    SH-4-54

    A) Comparison of BP-1-102 (1) or SH-4-54 against the top four from the new library in BTSC30M. ACS Med Chem Lett. 2013 Sep 8;4(11):1102-7.



    SH-4-54


     

    SH-4-54

    ACS Med Chem Lett. 2013 Sep 8;4(11):1102-7. (11):1102-7.
     

    SH-4-54

    ACS Med Chem Lett. 2013 Sep 8;4(11):1102-7.


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