Serotonin HCl

Alias: 5-HT HCl; 5-Hydroxytryptamine hydrochloride; 5-HT hydrochloride
Cat No.:V0993 Purity: ≥98%
Serotonin HCl (5-HT HCl; 5-Hydroxytryptamine), the hydrochloride salt of Serotonin, is an endogenous monoamine neurotransmitter found in the brain and also an endogenous agonist of 5-HT receptor.
Serotonin HCl Chemical Structure CAS No.: 153-98-0
Product category: 5-HT Receptor
This product is for research use only, not for human use. We do not sell to patients.
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Other Forms of Serotonin HCl:

  • Serotonin (5-Hydroxytryptamine; 5-HT)
Official Supplier of:
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Top Publications Citing lnvivochem Products
Purity & Quality Control Documentation

Purity: ≥98%

Product Description

Serotonin HCl (5-HT HCl; 5-Hydroxytryptamine), the hydrochloride salt of Serotonin, is an endogenous monoamine neurotransmitter found in the brain and also an endogenous agonist of 5-HT receptor.

Biological Activity I Assay Protocols (From Reference)
Targets
Human Endogenous Metabolite; Microbial Metabolite; serotonin
ln Vitro

In vitro activity: Serotonin hydrochloride is an endogenous 5-HT receptor agonist and a monoamine neurotransmitter in the central nervous system. Additionally, catechol O-methyltransferase (COMT), an enzyme that modulates pain perception, is inhibited by serotonin hydrochloride through non-competitive binding to the site bound by catechol substrates, with a binding affinity similar to that of catechol itself (Ki=?44 μM). The addition of 100 μM of serotonin hydrochloride reduces the COMT reaction velocity, according to the results[1].

ln Vivo
Serotonin hydrochloride generates a strong hypersensitivity in comparison to controls treated with saline (p<0.001)[1]. After DSS colitis is induced, IL-13-/-mice receiving serotonin hydrochloride show a markedly higher colonic 5-HT content than IL-13-/-mice receiving a vehicle[2].
Cell Assay
Peritoneal cavity cells from WT and IL-13-/- mice are harvested and cultured, either with or without dextran sodium sulfate (DSS) treatment, for the purpose of macrophage culture. Following a 24-hour treatment with lipopolysaccharides (LPS; 100 ng/mL) or serotonin hydrochloride (10–10 M), cells are plated at a concentration of 3.0×106 cells per milliliter. After being collected, the culture supernatant is kept at -80°C until the protein array system is used to determine the levels of cytokines[2].
Animal Protocol
Oral administration of 5% DSS in drinking water for five days induces diarrhea caused by dextran sodium sulfate (DSS). A different experiment involves injecting 100 mg/kg of serotonin hydrochloride (5-HTP) subcutaneously into IL-13-/-mice twice a day for 8 days, starting 3 days before DSS colitis is induced. In contrast, saline is used as a vehicle in the IL-13-/-modified mice. Upon reaching a predefined end point (such as losing more than 20% of their body weight or experiencing a notable decline in their physical state), animals are put to sleep before being put to death by cervical dislocation at the end of each experiment[2].
References

[1]. Serotonin-induced hypersensitivity via inhibition of catechol O-methyltransferase activity. Mol Pain. 2012 Apr 13;8:25.

[2]. Interleukin 13 and serotonin: linking the immune and endocrine systems in murine models of intestinal inflammation. PLoS One. 2013 Aug 28;8(8):e72774.

These protocols are for reference only. InvivoChem does not independently validate these methods.
Physicochemical Properties
Molecular Formula
C10H13CLN2O
Molecular Weight
212.68
Exact Mass
212.07
CAS #
153-98-0
Appearance
Gray to dark gray powder
SMILES
C1=CC2=C(C=C1O)C(=CN2)CCN.Cl
InChi Key
MDIGAZPGKJFIAH-UHFFFAOYSA-N
InChi Code
InChI=1S/C10H12N2O.ClH/c11-4-3-7-6-12-10-2-1-8(13)5-9(7)10;/h1-2,5-6,12-13H,3-4,11H2;1H
Chemical Name
3-(2-aminoethyl)-1H-indol-5-ol;hydrochloride
Synonyms
5-HT HCl; 5-Hydroxytryptamine hydrochloride; 5-HT hydrochloride
Storage

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

Shipping Condition
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
Solubility Data
Solubility (In Vitro)
DMSO: 43~125 mg/mL (202.2~587.7 mM)
Water: ~43 mg/mL (~202.2 mM)
Ethanol: ~2 mg/mL (~9.4 mM)
Preparing Stock Solutions 1 mg 5 mg 10 mg
1 mM 4.7019 mL 23.5095 mL 47.0190 mL
5 mM 0.9404 mL 4.7019 mL 9.4038 mL
10 mM 0.4702 mL 2.3509 mL 4.7019 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.

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Method for preparing DMSO stock solution mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation:Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.

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Biological Data
  • Inhibition of COMT with serotonin. Mol Pain . 2012 Apr 13:8:25.
  • In silico and in vitro binding of serotonin and COMT. Mol Pain . 2012 Apr 13:8:25.
  • Reduction of serotonin-induced hypersensitivity by SAM and β2/β3antagonism. Mol Pain . 2012 Apr 13:8:25.
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