Size | Price | Stock | Qty |
---|---|---|---|
50mg |
|
||
100mg |
|
||
250mg |
|
||
500mg |
|
||
1g |
|
||
5g |
|
||
Other Sizes |
|
Purity: ≥98%
Serotonin HCl (5-HT HCl; 5-Hydroxytryptamine), the hydrochloride salt of Serotonin, is an endogenous monoamine neurotransmitter found in the brain and also an endogenous agonist of 5-HT receptor.
Targets |
Human Endogenous Metabolite; Microbial Metabolite; serotonin
|
|
---|---|---|
ln Vitro |
|
|
ln Vivo |
|
|
Cell Assay |
Peritoneal cavity cells from WT and IL-13-/- mice are harvested and cultured, either with or without dextran sodium sulfate (DSS) treatment, for the purpose of macrophage culture. Following a 24-hour treatment with lipopolysaccharides (LPS; 100 ng/mL) or serotonin hydrochloride (10–10 M), cells are plated at a concentration of 3.0×106 cells per milliliter. After being collected, the culture supernatant is kept at -80°C until the protein array system is used to determine the levels of cytokines[2].
|
|
Animal Protocol |
Oral administration of 5% DSS in drinking water for five days induces diarrhea caused by dextran sodium sulfate (DSS). A different experiment involves injecting 100 mg/kg of serotonin hydrochloride (5-HTP) subcutaneously into IL-13-/-mice twice a day for 8 days, starting 3 days before DSS colitis is induced. In contrast, saline is used as a vehicle in the IL-13-/-modified mice. Upon reaching a predefined end point (such as losing more than 20% of their body weight or experiencing a notable decline in their physical state), animals are put to sleep before being put to death by cervical dislocation at the end of each experiment[2].
|
|
References |
Molecular Formula |
C10H13CLN2O
|
|
---|---|---|
Molecular Weight |
212.68
|
|
Exact Mass |
212.07
|
|
CAS # |
153-98-0
|
|
Appearance |
Gray to dark gray powder
|
|
SMILES |
C1=CC2=C(C=C1O)C(=CN2)CCN.Cl
|
|
InChi Key |
MDIGAZPGKJFIAH-UHFFFAOYSA-N
|
|
InChi Code |
InChI=1S/C10H12N2O.ClH/c11-4-3-7-6-12-10-2-1-8(13)5-9(7)10;/h1-2,5-6,12-13H,3-4,11H2;1H
|
|
Chemical Name |
3-(2-aminoethyl)-1H-indol-5-ol;hydrochloride
|
|
Synonyms |
|
|
Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
|
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
Solubility (In Vitro) |
|
---|
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 4.7019 mL | 23.5095 mL | 47.0190 mL | |
5 mM | 0.9404 mL | 4.7019 mL | 9.4038 mL | |
10 mM | 0.4702 mL | 2.3509 mL | 4.7019 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Inhibition of COMT with serotonin. Mol Pain . 2012 Apr 13:8:25. td> |
In silico and in vitro binding of serotonin and COMT. Mol Pain . 2012 Apr 13:8:25. td> |
Reduction of serotonin-induced hypersensitivity by SAM and β2/β3antagonism. Mol Pain . 2012 Apr 13:8:25. td> |