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Purity: ≥98%
SEA0400 is a Na+/Ca2+ exchanger 1 inhibitor. SEA0400 prevents dopaminergic neurotoxicity in an MPTP mouse model of Parkinson's disease. SEA0400 reduces calcium overload induced by ischemia and reperfusion in mouse ventricular myocytes. SEA0400 attenuates sodium nitroprusside-induced apoptosis in cultured rat microglia.
| ln Vitro |
SEA0400 prevents astrocytes, microglia, and cultured neurons from absorbing 45Ca2+ in a Na+-dependent manner. SEA0400's IC50 values for neurons, astrocytes, and microglia are 33 nM, 5.0 nM, and 8.3 nM, respectively[1]. In an extracellular Ca2+-dependent manner, SEA0400 inhibits the effects of sodium nitroprusside (SNP) on phosphorylation of ERK and p38 MAPK as well as the generation of reactive oxygen species (ROS)[2].
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| ln Vivo |
In rats under anesthesia, SEA0400 (3 mg/kg + 3 mg/kg/h for 2 h, iv) reduces the infarct volume in the cerebral cortex and striatum but has no effect on the mean regional cortical blood flow[1]. When MPTP is administered to C57BL/6J mice, SEA0400 shields them from the dopaminergic neurotoxicity that is measured by motor deficits, tyrosine hydroxylase immunoreactivity in the substantia nigra and striatum, and dopamine levels in the midbrain and striatum[3].
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| Animal Protocol |
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| References |
[1]. Matsuda T, et al. SEA0400, a novel and selective inhibitor of the Na+-Ca2+ exchanger, attenuates reperfusion injury in the in vitro and in vivo cerebral ischemic models. J Pharmacol Exp Ther. 2001 Jul;298(1):249-56.
[2]. Nashida T, et al. The specific Na(+)/Ca(2+) exchange inhibitor SEA0400 prevents nitric oxide-induced cytotoxicity in SH-SY5Y cells. Neurochem Int. 2011 Aug;59(1):51-8. [3]. Ago Y, et al. SEA0400, a specific Na+/Ca2+ exchange inhibitor, prevents dopaminergic neurotoxicity in an MPTP mouse model of Parkinson's disease. Neuropharmacology. 2011 Dec;61(8):1441-51 |
| Molecular Formula |
C21H19F2NO3
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| Molecular Weight |
371.13
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| CAS # |
223104-29-8
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| SMILES |
FC1C([H])=C([H])C(=C([H])C=1C([H])([H])OC1C([H])=C([H])C(=C([H])C=1[H])OC1C([H])=C([H])C(=C([H])C=1N([H])[H])OC([H])([H])C([H])([H])[H])F
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| Synonyms |
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
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| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: 2.5 mg/mL (6.73 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (6.73 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.6945 mL | 13.4724 mL | 26.9447 mL | |
| 5 mM | 0.5389 mL | 2.6945 mL | 5.3889 mL | |
| 10 mM | 0.2694 mL | 1.3472 mL | 2.6945 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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