| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 25mg |
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| 50mg |
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| 100mg |
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| 250mg |
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| Other Sizes |
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| ln Vitro |
Compound 26, SBP-7455, inhibited MDA-MB-468 cell proliferation with an IC50 of 0.3 μM over a 72-hour period. In TNBC cells with autophagy Sy, SBP-7455 reduces the autophagy flux caused by starvation [1].
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| ln Vivo |
The mouse model received a single dose of 30 mg/kg of SBP-7455 (Compound 26). The Tmax and Cmax of SBP-7455 are 990 nM, 1.7 hours, and around an hour, respectively. The feeding concentration of SBP-7455 stayed above the ULK1 IC50 for over 4 hours following the first determination [1]. Two hours after mice were given oral gavage of SBP-7455 (compound 26) at a dose of 10 mg/kg, elephant samples were taken. According to the findings, SBP-7455 synchronizes the levels of ULK1 and total ATG13 and has a potent inhibitory effect on pATG13 (Ser318) [1].
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| References |
[1]. Huiyu Ren, et al. Design, Synthesis, and Characterization of an Orally Active Dual-Specific ULK1/2 Autophagy Inhibitor that Synergizes with the PARP Inhibitor Olaparib for the Treatment of Triple-Negative Breast Cancer. J Med Chem. 2020 Dec 10;63(23):14609-14625
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| Molecular Formula |
C16H17F3N4O2
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|---|---|
| Molecular Weight |
354.3332
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| Exact Mass |
354.1304
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| Elemental Analysis |
C, 54.24; H, 4.84; F, 16.09; N, 15.81; O, 9.03
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| CAS # |
1884222-74-5
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| SMILES |
FC(C1=C([H])N=C(N([H])C2C([H])=C([H])C(=C(C=2[H])OC([H])([H])[H])OC([H])([H])[H])N=C1N([H])C1([H])C([H])([H])C1([H])[H])(F)F
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| Chemical Name |
N4-cyclopropyl-N2-(3,4-dimethoxyphenyl)-5-(trifluoromethyl)pyrimidine-2,4-diamine
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| Synonyms |
SBP 7455SBP7455 SBP-7455
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~125 mg/mL (~352.78 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (5.87 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (5.87 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.8222 mL | 14.1111 mL | 28.2223 mL | |
| 5 mM | 0.5644 mL | 2.8222 mL | 5.6445 mL | |
| 10 mM | 0.2822 mL | 1.4111 mL | 2.8222 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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