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    (S)-10-Hydroxycamptothecin
    (S)-10-Hydroxycamptothecin

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    This product is for research use only, not for human use. We do not sell to patients.
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    InvivoChem Cat #: V1421
    CAS #: 19685-09-7Purity ≥98%

    Description: 10-Hydroxycamptothecin (also abbreviated as 10HCPT; 10OHCPT; HCPT; 10 HCPT), the 10-hydroxylated form of camptothecin, is a natural product and topoisomerase I inhibitor with potential anticancer activity. It dose-dependently and significantly inhibits the migration of HMEC with an IC50 of 0.63 μM. The proliferation of human microvascular endothelial cells (HMEC) and seven human tumor cell lines were detected by SRB assay, resulting in study the antiangiogenic potential of 10-hydroxycamptothecin (HCPT), ,and the endothelial cell migration and tube formation were assessed using two in vitro model systems.

    References: Oncol Rep. 2006 May;15(5):1273-9; Mol Pharmacol. 2000 Feb;57(2):243-51.

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    Molecular Weight (MW)364.35 
    FormulaC20H16N2O5 
    CAS No.19685-09-7 
    Storage-20℃ for 3 years in powder form
    -80℃ for 2 years in solvent
    Solubility (In vitro)DMSO: 8 mg/mL (22.0 mM) 
    Water: <1 mg/mL
    Ethanol: <1 mg/mL
    Solubility (In vivo)30% PEG400+0.5% Tween80+5% Propylene glycol: 30mg/mL
    Synonyms10-HCPT; Hydroxycamptothecin; 10OHCPT; HCPT; 10hydroxycamptothecine; 10HCPT; 10 HCPT; (S)10Hydroxycamptothecin; 10Hydroxy camptothecin; Hydroxycamptothecine; Camptothecin hydroxy10hydroxycamptothecin.


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    In Vitro

    In vitro activity: 10-Hydroxycamptothecin inhibits the growth of BT-20 and MDA-231 cell with IC50 of 34.3 nM and 7.27 nM, respectively, more potently than camptothecin (CPT) with IC50 of >500 nM. 10-Hydroxycamptothecin potently induces human topoisomerase I-mediated cleavable complex formation of pBR322 plasmid DNA with EC50 of 0.35 μM, displaying >50-fold potency than CPT with EC50 of 18.85 μM. 10-Hydroxycamptothecin treatment induces dose-dependent growth inhibition of human microvascular endothelial cells (HMEC) with IC50 of 0.31 μM, and significantly inhibits the migration of HMEC with IC50 of 0.63 μM. Treatment of HMEC cells with 10-Hydroxycamptothecin also results in a dose-dependent inhibition of tube formation with IC50 of 0.96 μM. 10-Hydroxycamptothecin (5-20 nM) significantly inhibits the proliferation of Colo 205 cells, arrests the cells in the G2 phase of the cell cycle and induces apoptosis through a caspase-3-dependent pathway.


    Cell Assay: Cells (MDA-231, and BT-20 cells) are exposed to various concentrations of 10-Hydroxycamptothecin for 72 hours. Cell growth is monitored by the standard MTT assay.

    In Vivo10-Hydroxycamptothecin treatment inhibits angiogenesis in a concentration-dependent fashion in the CAM model, with 95% inhibition at dose of 25 nM, more potently than suramin which at 125 nM inhibits angiogenesis only by 60%. Oral administration of 10-Hydroxycamptothecin at low doses of 2.5-7.5 mg/kg every 2 days leads to significant growth inhibition of Colo 205 xenografts in mice without acute toxicity. LD50: Mice 104mg/kg (i.p.).  
    Animal modelBALB/c-nu mice transplanted subcutaneously with Colo 205 cells 
    Formulation & DosageDissolved in propylene glycol; 7.5 mg/kg; p.o. administration 
    References

    Oncol Rep. 2006 May;15(5):1273-9; Mol Pharmacol. 2000 Feb;57(2):243-51. 


    These protocols are for reference only. InvivoChem does not independently validate these methods.

    10-Hydroxycamptothecin (also abbreviated as 10HCPT; 10OHCPT; HCPT; 10 HCPT), the 10-hydroxylated form of camptothecin, is a natural product and topoisomerase I inhibitor with potential anticancer activity.

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