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Purity: ≥98%
10-Hydroxycamptothecin (also abbreviated as 10HCPT; 10OHCPT; HCPT; 10 HCPT), the 10-hydroxylated form of camptothecin, is a natural product and topoisomerase I inhibitor with potential anticancer activity. It significantly and dose-dependently inhibits HMEC migration, with an IC50 of 0.63 μM. The SRB assay was used to detect the proliferation of seven human tumor cell lines and human microvascular endothelial cells (HMEC). This led to an investigation into the antiangiogenic potential of 10-hydroxycamptothecin (HCPT), and two in vitro model systems were used to evaluate endothelial cell migration and tube formation.
| Targets |
Topoisomerase I
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| ln Vitro |
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| ln Vivo |
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| Cell Assay |
For 72 hours, different concentrations of 10-Hydroxycamptothecin are applied to cells. The conventional MTT assay is used to track cell growth.
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| Animal Protocol |
BALB/c-nu mice transplanted subcutaneously with Colo 205 cells
~7.5 mg/kg Orally once per two days BALB/c-nu mice transplanted subcutaneously with Colo 205 cells |
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| References |
| Molecular Formula |
C20H16N2O5
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| Molecular Weight |
364.35
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| Exact Mass |
364.11
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| Elemental Analysis |
C, 65.93; H, 4.43; N, 7.69; O, 21.96
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| CAS # |
19685-09-7
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| Related CAS # |
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| Appearance |
Light yellow solid powder
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| SMILES |
CC[C@@]1(C2=C(COC1=O)C(=O)N3CC4=C(C3=C2)N=C5C=CC(=CC5=C4)O)O
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| InChi Key |
HAWSQZCWOQZXHI-FQEVSTJZSA-N
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| InChi Code |
InChI=1S/C20H16N2O5/c1-2-20(26)14-7-16-17-11(5-10-6-12(23)3-4-15(10)21-17)8-22(16)18(24)13(14)9-27-19(20)25/h3-7,23,26H,2,8-9H2,1H3/t20-/m0/s1
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| Chemical Name |
(19S)-19-ethyl-7,19-dihydroxy-17-oxa-3,13-diazapentacyclo[11.8.0.02,11.04,9.015,20]henicosa-1(21),2(11),3,5,7,9,15(20)-heptaene-14,18-dione
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| Synonyms |
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
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| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
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| Solubility (In Vivo) |
Solubility in Formulation 1: 2.5 mg/mL (6.86 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 2.5 mg/mL (6.86 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: 30% PEG400+0.5% Tween80+5% Propylene glycol : 30mg/mL |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.7446 mL | 13.7231 mL | 27.4461 mL | |
| 5 mM | 0.5489 mL | 2.7446 mL | 5.4892 mL | |
| 10 mM | 0.2745 mL | 1.3723 mL | 2.7446 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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