RIPA-56

Alias: RIPA-56; RIPA-56; RIPA-56
Cat No.:V3403 Purity: ≥98%
RIPA-56 is a novel, highly potent, selective, and metabolically stable inhibitor of receptor-interacting protein 1 (RIP1) with anIC50of 13 nM.
RIPA-56 Chemical Structure CAS No.: 1956370-21-0
Product category: RIP kinase
This product is for research use only, not for human use. We do not sell to patients.
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Purity & Quality Control Documentation

Purity: ≥98%

Product Description

RIPA-56 is a novel, highly potent, selective, and metabolically stable inhibitor of receptor-interacting protein 1 (RIP1) with an IC50 of 13 nM. Tumor necrosis factor alpha (TNFα)-induced mortality and multiorgan damage were successfully decreased by RIPA-56. RIPA-56 is more effective in animal model studies, much more stable in vivo, and potent in both human and murine cells compared to other known RIP1 inhibitors.

Biological Activity I Assay Protocols (From Reference)
Targets
RIP1 (IC50 = 13 nM)
ln Vitro
RIPA-56 shows efficient inhibition of RIP1 kinase activity, with an IC50 of 13 nM.At a 10 µM concentration, it showed no evidence of inhibiting RIP3 kinase activity. Based on the 13 nM RIP1 ADP-Glo activity, RIPA-56 does not inhibit IDO activity at a concentration of 200 µM, which is thought to have a 10,000-fold selectivity window[1].
ln Vivo
RIPA-56 has an impressive in vivo PK profile in mice, with a 3.1 h half-life, 22% oral bioavailability (PO), and 100% bioavailability from intraperitoneal injection (IP). RIPA-56 is very good at crossing the blood–brain barrier. RIPA-56 effectively decreased mortality and multi-organ damage brought on by tumor necrosis factor alpha (TNFα) in the SIRS mouse disease model[1].
Cell Assay
Cell necrosis assay is performed in 96-well cell culture plate. Each well receives 3,000 cells, which are then cultured at 37°C over night. For 24 hours, HT-29 cells are treated with 20 ng/mL TNF, 100 nM Smac Mimetics, 20 μM z-VAD-FMK, and RIPA-56. 20 ng/mL TNFα/20 μM z-VAD-FMK, and RIPA-56 are applied to L929 cells and left on the cells for 6 hours. The Cell Titer-Glo Luminescent Cell Viability Assay kit[1] is used to calculate the cell survival ratio.
References

[1]. J Med Chem . 2017 Feb 9;60(3):972-986.

These protocols are for reference only. InvivoChem does not independently validate these methods.
Physicochemical Properties
Molecular Formula
C13H19NO2
Molecular Weight
221.29546380043
Exact Mass
221.14
Elemental Analysis
C, 70.56; H, 8.65; N, 6.33; O, 14.46
CAS #
1956370-21-0
Appearance
Solid powder
SMILES
CCC(C)(C)C(=O)N(CC1=CC=CC=C1)O
InChi Key
AVYVHIKSFXVDBG-UHFFFAOYSA-N
InChi Code
InChI=1S/C13H19NO2/c1-4-13(2,3)12(15)14(16)10-11-8-6-5-7-9-11/h5-9,16H,4,10H2,1-3H3
Chemical Name
N-benzyl-N-hydroxy-2,2-dimethylbutanamide
Synonyms
RIPA-56; RIPA-56; RIPA-56
HS Tariff Code
2934.99.9001
Storage

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

Shipping Condition
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
Solubility Data
Solubility (In Vitro)
DMSO: ~44 mg/mL (~198.8 mM)
Ethanol: ~ 44 mg/mL (~198.8 mM)
Preparing Stock Solutions 1 mg 5 mg 10 mg
1 mM 4.5188 mL 22.5938 mL 45.1875 mL
5 mM 0.9038 mL 4.5188 mL 9.0375 mL
10 mM 0.4519 mL 2.2594 mL 4.5188 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.

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What volume of a given 10 mM stock solution is required to make 25 ml of a 25 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=25 μM, V2=25 ml and V1 is the unknown:
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Note: Chemical formula is case sensitive: C12H18N3O4  c12h18n3o4
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Calculation results

Working concentration mg/mL;

Method for preparing DMSO stock solution mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation:Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
             (2) Be sure to add the solvent(s) in order.

Biological Data
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