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5mg |
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25mg |
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Purity: ≥98%
RG108, also known as N-Phthalyl-L-tryptophan, is a novel non-nucleoside inhibitor of DNA methyltransferase ((DNMT) with potential antitumor activity. It inhibits DNMT with an IC50 of 115 nM in a cell-free assay.
ln Vitro |
RG108 effectively blocks DNA methyltransferases in vitro and does not cause covalent enzyme trapping in human cell lines. Incubating cells with low micromolar doses of RG108 resulted in considerable demethylation of genomic DNA without any observable harm. Interestingly, RG108 causes demethylation and reactivation of tumor suppressor genes, but it does not change methylation of centromeric satellite sequences [1]. In another study, the synthesis and in vitro analysis of biotinylated RG108 conjugates were investigated to determine interactions with DNA methyltransferases [2]. A recent study demonstrated that RG108 might drastically inhibit DNA methyltransferase activity in SM-derived iPS cells compared with native SMs [3].
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ln Vivo |
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Animal Protocol |
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References |
[1]. Brueckner B, et al. Epigenetic reactivation of tumor suppressor genes by a novel small-molecule inhibitor of human DNA methyltransferases. Cancer Res. 2005 Jul 15;65(14):6305-11.
[2]. Schirrmacher E, et al. Synthesis and in vitro evaluation of biotinylated RG108: a high affinity compound for studying binding interactions with human DNA methyltransferases. Bioconjug Chem. 2006 Mar-Apr;17(2):261-6. [3]. Pasha Z, et al. Efficient non-viral reprogramming of myoblasts to stemness with a single small molecule to generate cardiac progenitor cells. PLoS One. 2011;6(8):e23667 |
Molecular Formula |
C19H14N2O4
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Molecular Weight |
334.33
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CAS # |
48208-26-0
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Related CAS # |
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SMILES |
O=C1C2=C([H])C([H])=C([H])C([H])=C2C(N1[C@]([H])(C(=O)O[H])C([H])([H])C1=C([H])N([H])C2=C([H])C([H])=C([H])C([H])=C12)=O
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InChi Key |
HPTXLHAHLXOAKV-INIZCTEOSA-N
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InChi Code |
InChI=1S/C19H14N2O4/c22-17-13-6-1-2-7-14(13)18(23)21(17)16(19(24)25)9-11-10-20-15-8-4-3-5-12(11)15/h1-8,10,16,20H,9H2,(H,24,25)/t16-/m0/s1
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Chemical Name |
(S)-2-(1,3-dioxoisoindolin-2-yl)-3-(1H-indol-3-yl)propanoic acid.
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Synonyms |
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
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Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
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Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 2.9911 mL | 14.9553 mL | 29.9106 mL | |
5 mM | 0.5982 mL | 2.9911 mL | 5.9821 mL | |
10 mM | 0.2991 mL | 1.4955 mL | 2.9911 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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