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Purity: ≥98%
Quercetagetin (6-Hydroxyquercetin) is a naturally occurring flavonoid and the major component isolated from Citrus unshiu (C. unshiu) peel, with anti-inflammatory and anticancer activity. It acts as an inhibitor of multple kinases such as pim-1 kinase with IC50 of 0.34 μM.
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| ln Vitro |
Quercetagetin inhibits PIM2, PKA, and RSK2 at IC50s of 3.45, 21.2, and 2.82 μM, respectively [2]. Quercetagetin (0.1, 1, 10, and 100 μM, 72 hours) inhibited the proliferation of RWPE2 prostate cancer cells with an average ED50 of 3.8 μM [2].
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| ln Vivo |
Quercetagetin dramatically reduces the growth of UVB-induced skin cancer. When applied topically to mouse skin, Quercetagetin at concentrations of 4 or 20 nmol decreased tumor incidence by 32.0% and 46.7%, respectively [3].
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| Cell Assay |
Cell Viability Assay[2]
Cell Types: RWPE2 prostate cancer cells Tested Concentrations: 0.1, 1, 10, and 100 μM Incubation Duration: 72 hrs (hours) Experimental Results: Inhibited growth of RWPE2 prostate cancer cells with average ED50 is 3.8 μM. |
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| Animal Protocol |
Animal/Disease Models: SKH-1 hairless mice model[3]
Doses: 4 or 20 nmol Route of Administration: Topical application; 28 weeks Experimental Results: Inhibited UVB-induced skin tumorigenesis in SKH-1 hairless mice models. Delayed the development of tumors and decreased tumor volumes in an SKH-1 hairless mice model. |
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| References |
[1]. Yang X, et al. Isolation and identification of an antioxidant flavonoid compound from citrus-processing by-product. J Sci Food Agric. 2011 Aug 15;91(10):1925-7.
[2]. Holder S, et al. Characterization of a potent and selective small-molecule inhibitor of the PIM1 kinase. Mol Cancer Ther. 2007 Jan;6(1):163-72. [3]. Baek S, et al. Structural and functional analysis of the natural JNK1 inhibitor quercetagetin. J Mol Biol. 2013 Jan 23;425(2):411-23. |
| Molecular Formula |
C₁₅H₁₀O₈
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| Molecular Weight |
318.24
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| CAS # |
90-18-6
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| SMILES |
O1C(=C(C(C2C(=C(C(=C([H])C1=2)O[H])O[H])O[H])=O)O[H])C1C([H])=C([H])C(=C(C=1[H])O[H])O[H]
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| Synonyms |
6-Hydroxyquercetin
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~125 mg/mL (~392.79 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (6.54 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (6.54 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.1423 mL | 15.7114 mL | 31.4228 mL | |
| 5 mM | 0.6285 mL | 3.1423 mL | 6.2846 mL | |
| 10 mM | 0.3142 mL | 1.5711 mL | 3.1423 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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