| Size | Price | Stock | Qty |
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| 50mg |
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| 100mg |
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| Other Sizes |
| Targets |
Sperm acrosin, trypsin (substrate for trypsin activity assays). 4-Nitrophenyl 4-guanidinobenzoate hydrochloride is a substrate for trypsin and related serine proteases, and a potent inhibitor of sperm acrosin. The guanidinobenzoate moiety is recognized by the active site of serine proteases, and the ester bond is cleaved by the enzyme. The compound also inhibits phenoloxidase enzyme and delays embryo chorionic membrane hardening.
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| ln Vitro |
4-Nitrophenyl 4-guanidinobenzoate hydrochloride is a trypsin substrate and a potent inhibitor of sperm acrosin. It inhibits phenoloxidase enzyme and delays embryo chorionic membrane hardening. The compound is used as a biochemical tool for enzyme assays. It is more water-soluble than 4-methylumbelliferyl 4-guanidinobenzoate and is preferred in spectrophotometric assays for trypsin and related serine proteases.
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| ln Vivo |
4-Nitrophenyl 4-guanidinobenzoate hydrochloride is used as a biochemical tool for enzyme assays, not for in vivo pharmacological studies. It is not intended for therapeutic use. The compound's activity is limited to in vitro enzyme assays and sperm function studies.
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| Enzyme Assay |
Spectrophotometric enzyme assays are performed by incubating 4-nitrophenyl 4-guanidinobenzoate hydrochloride with the target enzyme (e.g., trypsin, acrosin) in appropriate buffer conditions (e.g., Tris-HCl pH 8.0, with or without calcium). Enzymatic hydrolysis releases 4-nitrophenol, which is measured at 405 nm. The increase in absorbance over time is monitored, and enzyme activity is calculated from the rate of absorbance increase using the molar extinction coefficient of 4-nitrophenol.
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| Cell Assay |
4-Nitrophenyl 4-guanidinobenzoate hydrochloride is not typically used in cell-based assays; it is a biochemical reagent for enzyme activity measurement. It may be used in sperm function assays to assess acrosin activity. In these assays, spermatozoa are incubated with the substrate, and the release of 4-nitrophenol is measured to assess acrosin activity and acrosome reaction.
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| Animal Protocol |
Not applicable (biochemical reagent; not used in animal experiments). 4-Nitrophenyl 4-guanidinobenzoate hydrochloride is used in in vitro enzyme assays and sperm function studies. It is not administered to animals for pharmacological studies.
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| ADME/Pharmacokinetics |
4-Nitrophenyl 4-guanidinobenzoate hydrochloride is a biochemical reagent with no pharmacological pharmacokinetic properties. It is soluble in aqueous buffers and DMSO. Stored at appropriate conditions (e.g., -20°C, protected from light and moisture) to prevent hydrolysis. The compound should be prepared fresh for assays to ensure accurate results.
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| Toxicity/Toxicokinetics |
4-Nitrophenyl 4-guanidinobenzoate hydrochloride is a biochemical reagent handled with standard laboratory safety practices. It is not intended for in vivo administration. The compound is used in enzyme assays at micromolar concentrations. Appropriate personal protective equipment (PPE) should be used when handling the compound.
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| References | |
| Additional Infomation |
See other relationships...
4-Nitrophenyl 4-guanidinobenzoate hydrochloride (pNPGB) is a research-grade biochemical reagent for enzyme activity assays. Molecular formula: C14H13ClN4O4, molecular weight: 336.73. Purity: ≥98% (TLC). CAS: 19135-17-2. It is a trypsin substrate and sperm acrosin inhibitor. Synonyms: NPGB hydrochloride. For research use only, not for human therapeutic use. |
| Molecular Formula |
C14H13CLN4O4
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|---|---|
| Molecular Weight |
336.73
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| Exact Mass |
336.063
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| CAS # |
19135-17-2
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| Related CAS # |
21658-26-4 (Parent)
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| PubChem CID |
2853494
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| Appearance |
White to off-white solid powder
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| Density |
1.44g/cm3
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| Boiling Point |
559.7ºC at 760mmHg
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| Melting Point |
241-243ºC
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| Flash Point |
292.3ºC
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| Vapour Pressure |
1.47E-12mmHg at 25°C
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| LogP |
4.317
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
23
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| Complexity |
429
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| Defined Atom Stereocenter Count |
0
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| SMILES |
Cl.N/C(=N/C1C=CC(C(OC2C=CC([N+]([O-])=O)=CC=2)=O)=CC=1)/N
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| InChi Key |
PKSBDZOBYIKNGY-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C14H12N4O4.ClH/c15-14(16)17-10-3-1-9(2-4-10)13(19)22-12-7-5-11(6-8-12)18(20)21;/h1-8H,(H4,15,16,17);1H
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| Chemical Name |
(4-nitrophenyl) 4-(diaminomethylideneamino)benzoate;hydrochloride
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO :~100 mg/mL (~296.97 mM; with sonication (<80°C))
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 5 mg/mL (14.85 mM) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one),clear solution; with ultrasonication.
For example, if 1 mL of working solution is to be prepared,you can add 100 μL of 50.0 mg/mL clear DMSO stock solution and add it to 400 μL PEG300 and mix well. Then add 50 μL Tween-80 to the above system and mix well. Then continue to add 450 μL of physiological saline to make up to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 5 mg/mL (14.85 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one),clear solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared,you can add 100 μL of 50.0 mg/mL clear DMSO stock solution and add it to 900 μL of 20% SBE-β-CD saline solution and mix well. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. View More
Solubility in Formulation 3: 5 mg/mL (14.85 mM) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one),clear solution; with ultrasonication. |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.9697 mL | 14.8487 mL | 29.6974 mL | |
| 5 mM | 0.5939 mL | 2.9697 mL | 5.9395 mL | |
| 10 mM | 0.2970 mL | 1.4849 mL | 2.9697 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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