| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
|
||
| Other Sizes |
| Targets |
Human Endogenous Metabolite
Lignoceric acid targets fatty acid metabolic pathways; it is a substrate for enzymes involved in fatty acid oxidation, elongation, and incorporation into complex lipids; it does not have a specific pharmacological receptor but is a metabolic intermediate and structural lipid component. |
|---|---|
| ln Vitro |
Normal peroxisomal metabolism is shown by the exothermic heat flux in HepG2 peroxisomes produced by lignoceric acid [1].
In vitro, Lignoceric acid is used in cell culture studies to investigate fatty acid metabolism, lipid synthesis, and the effects of VLCFAs on cellular function; it is incorporated into cellular lipids and can modulate membrane properties and signaling pathways. |
| ln Vivo |
In vivo, Lignoceric acid is a normal constituent of mammalian tissues, particularly in the brain where it is a component of cerebrosides and sulfatides; its levels are elevated in X-linked adrenoleukodystrophy (X-ALD) due to impaired peroxisomal β-oxidation; it is used as a biomarker for VLCFA accumulation.
|
| Enzyme Assay |
Non-cellular assay: Lignoceric acid is analyzed by gas chromatography (GC) or LC-MS following derivatization to fatty acid methyl esters (FAMEs); it is used as a standard for the quantification of VLCFAs in biological samples; its concentration is determined by comparison to internal standards in analytical methods.
|
| Cell Assay |
Cellular assays: Cells (e.g., fibroblasts from X-ALD patients or control subjects) are cultured with Lignoceric acid or in medium containing the compound; cellular lipid extracts are analyzed by GC or LC-MS to measure VLCFA levels; effects on cell viability, oxidative stress, and gene expression are assessed.
|
| Animal Protocol |
In vivo animal experiments: Lignoceric acid is administered to animal models or used as a dietary supplement to study VLCFA metabolism; its levels in plasma and tissues are measured to assess peroxisomal function; in X-ALD model mice, Lignoceric acid levels are monitored as a biomarker of disease progression and therapeutic efficacy.
|
| ADME/Pharmacokinetics |
Pharmacokinetic properties: Lignoceric acid is a lipophilic fatty acid that is absorbed from the diet, incorporated into lipoproteins, and distributed to tissues; it is metabolized by peroxisomal β-oxidation; its levels are regulated by synthesis, dietary intake, and degradation.
|
| Toxicity/Toxicokinetics |
Toxicological profile: Lignoceric acid is a naturally occurring fatty acid with low toxicity at physiological levels; elevated levels are associated with X-ALD and other peroxisomal disorders; it is not considered toxic per se but its accumulation is pathogenic in the context of metabolic disease.
|
| References |
|
| Additional Infomation |
Tetracosanoic acid (TCA) is a C24 straight-chain saturated fatty acid. It is a volatile oil component, a plant metabolite, a human metabolite, and a metabolite of the dark-colored water flea (Daphnia tenebrosa). It is a long-chain fatty acid and also a straight-chain saturated fatty acid. It is the conjugate acid of TCA. TCA has been reported to exist in hybrid amaranth (Amaranthus hybridus), shield-leaved amaranth (Tornabea scutellifera), and other organisms with relevant data. Cinnamic acid is a saturated fatty acid with a 24-carbon skeleton. Cinnamic acid is naturally found in wood tar, various cerebrosides, and in small amounts in most natural fats. Cinnamic acid is a metabolite found in or produced by the yeast Saccharomyces cerevisiae.
Other information: Lignoceric acid has a molecular formula of C₂₄H₄₈O₂ and a molecular weight of 368.64; it is used as a biomarker for the diagnosis and monitoring of X-linked adrenoleukodystrophy; it is also studied in the context of sphingolipid metabolism and neurodegenerative diseases. |
| Molecular Formula |
C24H48O2
|
|---|---|
| Molecular Weight |
368.64
|
| Exact Mass |
368.365
|
| CAS # |
557-59-5
|
| Related CAS # |
Lignoceric acid-d47;68060-00-4;Lignoceric acid-d4-1;1219794-59-8;Lignoceric acid-d4-2;1219794-61-2;Lignoceric acid-d9;2415226-90-1;Lignoceric acid-d3;851073-55-7
|
| PubChem CID |
11197
|
| Appearance |
White to off-white solid powder
|
| Density |
0.9±0.1 g/cm3
|
| Boiling Point |
405.9±8.0 °C at 760 mmHg
|
| Melting Point |
84.2 °C
|
| Flash Point |
182.2±13.3 °C
|
| Vapour Pressure |
0.0±1.0 mmHg at 25°C
|
| Index of Refraction |
1.460
|
| LogP |
11.4
|
| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
2
|
| Rotatable Bond Count |
22
|
| Heavy Atom Count |
26
|
| Complexity |
275
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
CCCCCCCCCCCCCCCCCCCCCCCC(=O)O
|
| InChi Key |
QZZGJDVWLFXDLK-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C24H48O2/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-18-19-20-21-22-23-24(25)26/h2-23H2,1H3,(H,25,26)
|
| Chemical Name |
tetracosanoic acid
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
Ethanol :~8.33 mg/mL (~22.60 mM )
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 0.83 mg/mL (2.25 mM) (saturation unknown) in 10% EtOH + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 8.3 mg/mL clear EtOH + stock solution to 900 μL of corn oil and mix well. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.7127 mL | 13.5634 mL | 27.1267 mL | |
| 5 mM | 0.5425 mL | 2.7127 mL | 5.4253 mL | |
| 10 mM | 0.2713 mL | 1.3563 mL | 2.7127 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
