| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
The peptide is a substrate for AMPK and SIK (salt-inducible kinase). It does not bind to the peptide as a therapeutic target; rather, it serves as an artificial substrate for measuring the catalytic activity of these kinases in vitro.
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| ln Vitro |
In kinase activity assays, purified AMPK or SIK transfers a phosphate group from ATP to a specific serine/threonine residue within the AMARA peptide. This phosphorylation can be detected by radioactive (32P-ATP incorporation) or luminescence-based methods.
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| ln Vivo |
AMARA peptide is a research tool for in vitro experiments only. It is not administered in vivo, nor does it possess intrinsic biological activity in living organisms. Its utility is strictly limited to cell-free biochemical assays and is not intended for animal studies.
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| Enzyme Assay |
In a typical in vitro kinase assay: Purified AMPK or cell lysates containing the kinase are incubated with reaction buffer (MgCl2, ATP, and 32P-ATP or unlabeled ATP) and AMARA peptide substrate (10-100 uM). After 30 min at 30degC, the reaction is stopped. Phosphorylated peptide is collected onto phosphocellulose paper or a streptavidin-coated plate (if biotinylated). Radioactivity or luminescence is measured.
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| Cell Assay |
AMARA peptide is not used in live cell experiments due to its inability to cross the cell membrane. For cellular kinase activity, cells are lysed after treatment with modulators, and the lysates are then used in in vitro kinase assays with AMARA peptide to quantify AMPK or SIK activity.
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| Animal Protocol |
Not applicable. AMARA peptide is a synthetic substrate for cell-free biochemical assays and is not used in in vivo animal experiments. It is strictly an in vitro research tool for quantifying kinase activity in lysates or purified enzyme preparations.
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| ADME/Pharmacokinetics |
The peptide is stable in dry powder form at -20degC for extended periods. In aqueous solution, it is stable for short-term storage (days to weeks) at -80degC or -20degC. It is resistant to rapid non-specific proteolysis but may degrade over extended periods.
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| Toxicity/Toxicokinetics |
No toxicity is reported for AMARA peptide as it is not used in animals or cell culture at concentrations where toxicity would be relevant. Standard laboratory precautions for handling synthetic peptides apply.
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| References | |
| Additional Infomation |
The AMARA peptide sequence (AMARAASAAALARRR) is derived from the consensus phosphorylation motif of AMPK (φ-X-Arg-X-X-Ser/Thr-X-X-φ, where φ is hydrophobic). It is a widely used standard substrate for AMPK activity assays and appears in hundreds of published research studies.
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| Molecular Formula |
C64H116F3N27O18S
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|---|---|
| Molecular Weight |
1640.84
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| Related CAS # |
AMARA peptide;163560-19-8
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| Appearance |
White to off-white solid powder
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O :~50 mg/mL (~30.47 mM)
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| Solubility (In Vivo) |
Solubility in Formulation 1: 33.33 mg/mL (20.31 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 0.6094 mL | 3.0472 mL | 6.0944 mL | |
| 5 mM | 0.1219 mL | 0.6094 mL | 1.2189 mL | |
| 10 mM | 0.0609 mL | 0.3047 mL | 0.6094 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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