| Size | Price | Stock | Qty |
|---|---|---|---|
| 50mg |
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| Other Sizes |
| Targets |
Cholesterol is a major sterol in mammals, constituting 20-25% of the plasma membrane structural composition. It is an endogenous estrogen-related receptor alpha (ERRalpha) agonist. It regulates membrane fluidity, permeability, and the function of transporters and signaling proteins, including ion channels.
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|---|---|
| ln Vitro |
The amplitude of K currents in isolated IHCs is reversibly altered in a solution containing 1 mM water-soluble cholesterol[2].
In vitro, water-soluble cholesterol (100-400 uM) can be used to load cells with cholesterol, increasing cholesterol contents in the plasma membrane in a concentration-dependent manner. It is used to study the effects of cholesterol enrichment on membrane properties and cellular signaling pathways. |
| ln Vivo |
In vivo, cholesterol is a fundamental component of animal physiology. Water-soluble cholesterol is used in research to study lipoprotein metabolism, lipid rafts, and drug delivery. It can be administered to animal models to modulate cholesterol levels, but detailed in vivo efficacy studies are limited.
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| Enzyme Assay |
For in vitro binding and activity assays, water-soluble cholesterol is used to study cholesterol-protein interactions. For example, cells are treated with water-soluble cholesterol (e.g., 1 mM for 45 minutes) to load them with cholesterol, followed by cell lysis and protein analysis (e.g., Western blotting) to assess effects on signaling proteins.
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| Cell Assay |
For cell-based assays, cells are seeded in culture plates and treated with water-soluble cholesterol (e.g., 100-400 ug/mL) for 30-60 minutes. After treatment, cells are washed and analyzed for cholesterol content using fluorescent probes or biochemical assays. Viability can be assessed using standard MTT assays.
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| Animal Protocol |
For animal studies, water-soluble cholesterol can be administered to mice via intraperitoneal injection or oral gavage. A common formulation is a 33.33 mg/mL solution in PBS. Dosing is typically daily for several weeks. Blood and tissue samples are collected to measure cholesterol levels and assess metabolic effects.
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| ADME/Pharmacokinetics |
Water-soluble cholesterol is formulated with methyl-beta-cyclodextrin at approximately 40 mg of cholesterol per gram of product. It is highly water-soluble (up to 300 mg/mL), allowing for preparation of clear aqueous solutions. Its pharmacokinetics are complex, as cholesterol is incorporated into lipoproteins and distributed.
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| Toxicity/Toxicokinetics |
Cholesterol is generally recognized as safe (GRAS). Water-soluble cholesterol formulations are considered non-toxic at the concentrations used in cell culture (e.g., 100-400 uM). At high doses, cholesterol supplementation can contribute to hypercholesterolemia and related cardiovascular risks in animal models. For research use only.
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| References | |
| Additional Infomation |
Cholesterol (Water Soluble) is a research-grade reagent. It is typically provided as a solid powder containing ~40 mg cholesterol per gram and a balance of methyl-beta-cyclodextrin. It is stored at -20degC, protected from moisture and light. It is not intended for human therapeutic use.
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| Exact Mass |
1054.82
|
|---|---|
| Related CAS # |
Cholesterol;57-88-5
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| PubChem CID |
168007050
|
| Appearance |
White to off-white solid powder
|
| Hydrogen Bond Donor Count |
0
|
| Rotatable Bond Count |
29
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| Heavy Atom Count |
76
|
| Complexity |
1890
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| Defined Atom Stereocenter Count |
0
|
| SMILES |
CC(C)CCCC(C)C1CCC2C1(CCC3C2CC=C4C3(CCC(C4)OC(=O)CCCCC(=O)OCCOC(=O)CCCCC(=O)OC5CCC6(C7CCC8(C(C7CC=C6C5)CCC8C(C)CCCC(C)C)C)C)C)C
|
| InChi Key |
FRJKPSUWOMDJAH-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C68H110O8/c1-45(2)17-15-19-47(5)55-29-31-57-53-27-25-49-43-51(33-37-65(49,7)59(53)35-39-67(55,57)9)75-63(71)23-13-11-21-61(69)73-41-42-74-62(70)22-12-14-24-64(72)76-52-34-38-66(8)50(44-52)26-28-54-58-32-30-56(48(6)20-16-18-46(3)4)68(58,10)40-36-60(54)66/h25-26,45-48,51-60H,11-24,27-44H2,1-10H3
|
| Chemical Name |
6-O-[10,13-dimethyl-17-(6-methylheptan-2-yl)-2,3,4,7,8,9,11,12,14,15,16,17-dodecahydro-1H-cyclopenta[a]phenanthren-3-yl] 1-O-[2-[6-[[10,13-dimethyl-17-(6-methylheptan-2-yl)-2,3,4,7,8,9,11,12,14,15,16,17-dodecahydro-1H-cyclopenta[a]phenanthren-3-yl]oxy]-6-oxohexanoyl]oxyethyl] hexanedioate
|
| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
H2O :~50 mg/mL
|
|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 33.33 mg/mL (Infinity mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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