Size | Price | Stock | Qty |
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1mg |
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5mg |
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Other Sizes |
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Targets |
NSF/GluR2 interactions[1]
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ln Vitro |
Pep2m, myristoylated TFA (10 μM), inhibits AMPA receptor (AMPAR) potentiation mediated by PKMζ[1]. Myristoylated TFA, Pep2m, and LTP maintenance do not prevent the rise of PKMζ in hippocampus slices, suggesting that preventing NSF/GluR2 connections does not stop PKMζ synthesis from being induced[1]. Pep2m, myristoylated TFA, reverses persistent potentiation at both weakly stimulated and powerfully stimulated synapses that undergo synaptic tagging and capture, and prevents NSF/GluR2-mediated AMPAR trafficking [1].
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ln Vivo |
In the formalin test, paw withdrawal thresholds (PWTs) on nociceptive responses increase in response to Pep2m, myristoylated TFA (10 µg/20 µL)[2].
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Animal Protocol |
Animal/Disease Models: Female and male Long-Evans hooded rats (8 weeks)[2 ]
Doses: 10 µg (in 20 µL) Route of Administration: Intrathecal injection Experimental Results: Resulted in an increase in PWTs, in both male and female rats at various time points tested. |
References |
[1]. Yudong Yao, et al. PKMζ Maintains Late Long-Term Potentiation by N-Ethylmaleimide-Sensitive Factor/GluR2-Dependent Trafficking of Postsynaptic AMPA Receptors. J Neurosci. 2008 Jul 30; 28(31): 7820-7827.
[2]. Nicole C George, et al. Sex differences in the contributions of spinal atypical PKCs and downstream targets to the maintenance of nociceptive sensitization. Mol Pain. 2019; 15: 1744806919840582. |
Molecular Formula |
C65H119F3N18O16S
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Molecular Weight |
1497.83
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Related CAS # |
Pep2m, myristoylated;1423381-07-0
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
DMSO :~33.33 mg/mL (~22.25 mM)
H2O :~3.33 mg/mL (~2.22 mM) |
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Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (1.67 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C,1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (1.67 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 0.6676 mL | 3.3382 mL | 6.6763 mL | |
5 mM | 0.1335 mL | 0.6676 mL | 1.3353 mL | |
10 mM | 0.0668 mL | 0.3338 mL | 0.6676 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.